| Project/Area Number |
14370359
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
UENO Shinichi Kagoshima University University Hospital, Faculty of Medicine and Dentistry, assistant professor, 医学部・歯学部附属病院, 講師 (40322317)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AIKOU Takashi Kagoshima University, Graduate school of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60117471)
MARUYAMA Ikorou Kagoshima University, Graduate school of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (20082282)
AKIYAMA Shinichi Kagoshima University, Graduate school of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60117413)
TAKAO Sonshin Kagoshima University, Frontier Science Research Center, Professor, フロンティアサイエンス研究推進センター, 教授 (80171411)
|
|---|
| Project Period (FY) |
2002 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Keywords | hepatocarcinogenesis / TNFa / IL8 / ROS / roxithromicin / 肝細胞癌 / IL8 / TNF / Roxithromycin / ROS / IL6 / IL-6 / TNF-a / 1L-8 |
|---|
| Research Abstract |
1)In 42 human HCC samples, IL-8 expression and its biological and clinical significance was studied. Using RT-PCR to IL-8 mRNA, 32 HCC samples were positive of IL-8. IL-8 expression of tumor tissue was not correlated with its angiogenesis studied by CD34 immunostaining. Hence, the incidence of vessel involvement was much higher in IL-8 positive group than in IL-8 negative group (n=10). Thus, TNM staging of HCC in positive group was higher than that in negative group. Overall, it is concluded that IL-8 expression in HCC may affect tumor cell behavior more than angiogenesis.
2)In rat-hepatocrcinogenesis induced by Diethylnitrosamin (DEN), liver weight and cancer volume were increased at 17-week DEN administration. Moreover, tissue MDA level was increased, and tissue NF-κB activation and the increase of iNOS level were shown. TNP470 (50mg/kg) and Roxithromicin (RXM 100mg/kg) inhibited the NF-κB activation and the following iNOS and MDA level.
3)By using nude mouse- dorsal bag model, it is shown that RXM inhibited tumor angiogenesis of HepG2.
|
