Long-existent microchimerism induced by pregnancy and immunotolerance: application for transplantation
| Project/Area Number |
14370361
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
OHTO Hitoshi Fukushima Medical University, School of Medicine, Professor, 医学部, 教授 (20150279)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Takashi Fukushima Medical University, School of Medicine, Assistant, 医学部, 助手 (70347235)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | microchimerism / pregnancy / blood transfusion / transplantation / HLA / tolerance / SRY / alloimmunization / Y染色体 / HLAクラスII / 新生児 / 白血球除去 |
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| Research Abstract |
[Background] Established cellular habitation of donor origin is thought to be a candidate mechanism for transfusion-related immnomodulation in blood recipients. Moreover, pregnancy/delivery induced microchimerism can be established in the mother and the child, and may bring antigen specific immunotolerance in both.
[Materials and Methods] 1. Microchimerism detected by nested-PCR which targeting SRY gene on Y chromosome or HLA-DRB1 gene. 2. Microchimerism in women having a history of pregnancy/delivery and in women not having such a history. 3. Alloimmunization in patients awaiting kidney transplantation. 4. Alloimmunization to platelet antigens by pregnancy. 5. Alloimmunization of preterm newborns against antigens of blood donors and/or of the mother.
[Results] 1. Detection sensitivity DRY nested PCR was able to detect one to five male cells in one milliliter of female blood. However, HLA-DRB1-nested PCR had a 2-log or 3-log less sensitivity compared to SRY nested PCR. 2. When SRY nested PCR was used, michrochimerism was observed in 84% of women who delivered at least one male child, whereas only 3% of women who had no history of pregnancy. 3. Anti-HLA was detected in 17% (1171693) patients awaiting kidney transplantation. 4. 0.9%(223124,630) of pregnant women were sensitized against (a) platelet alloantigen(s). 5. No premature newborn infants who received transfusion(s) were alloimmunized to antigens of blood donors or of the mother.
[Conclusion] 1. SRY targeting PCR is the most sensitive to detect microchimerism. 2. Around 80% of women who have a pregnancy have a microchimerism derived from the fetus. 3.17% of adult patients awaiting kidney transplantation and 0.9% of pregnant women are sensitized to HLA antigens and platelet antigens, respectively, however, premature newborn infants are rarely alloimmunized.
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Report
(4 results)
Research Products
(25 results)
