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Development of specific vector containing FN-1 fused protein and SCCA promoter for esophageal cancer

Research Project

Project/Area Number 14370370
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OKUSHIBA Shunichi  Hokkaido University, Graduate School of Medicine, Asso.Prof., 大学院・医学研究科, 助教授 (70185536)

Co-Investigator(Kenkyū-buntansha) MIYAMOTO Masaki  Hokkaido University Hospital, University Hospital, Asit, 医学部・歯学部附属病院, 助手 (40333611)
KONDO Satoshi  Hokkaido University, Graduate School of Medicine, Asso.Prof, 大学院・医学研究科, 助教授 (30215454)
KATOH Hiroyuki  Hokkaido University, Graduate School of Medicine, Prof, 大学院・医学研究科, 教授 (80002369)
TADA Mitsuhiro  Hokkaido University, Institute For Genetic Medicine, Asso.Prof, 遺伝子病制御研究所, 助教授 (10241316)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥12,500,000 (Direct Cost: ¥12,500,000)
Keywordsesophageal squamous cell carcinoma / gene therapy / RCAS1 / caveolin-1 / adenoviralvector / SCCA2 promotein / SCCAプロモーター / 非ウイルスベクター / 遺伝子解析 / caveolin-1
Research Abstract

We screened several gene expressions in esophageal squamous cell carcinoma (ESCC) for the purpose of finding of cancer specific gene expression and development of therapeutic gene. Then we found that RCAS1 and caveolin-1 are potentially useful for the target of esophageal cancer. Subsequently, we constructed a shuttle vector to make adenoviral vectors in which SCCA2 promoter was introduced. An adenoviral vector which expresses proapoptotic gene KLAKLA2 driven by SCCA2 promoter was reconstructed. Ad-SCCAp-KLAKLAK induced apoptosis in SCCA2 producing cell lines LK2 and LC1, but not A549 or ABC-1 that did not produce SCCA2. Effects of gene therapy using Ad-SCCAp-KLAKLAK were observed in vivo SCCA2 producing tumors implanted on the hack of nude mice were successfully suppressed by Ad-SCCAp-KLAKLAK.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hashida H: "Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool"Br J Cancer. 90. 1252-1258 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gyhobu H: "Generation and Targeting of Human Tumor-Specific Tc1 and Th1 cells Transduced with a Lentivirus Containing a Chimeric Immunoglobulin."Cancer Res. 64. 1490-1495 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cho Y: "CD4+ and CD8+ T Cells Cooperate to Improve Prognosis of Patients with Esophageal Squamous Cell Carcinoma."Cancer Res. 63. 1555-1559 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura T: "Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissection."Oncogene. 23. 2385-2400 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi Y: "Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system."Experimental Cell Res. 293. 144-153 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Hashida: "Fusion of Tat protein transduction domain to poly-lysine as a new DNA delivery tool."British Journal of Cancer. 90. 1252-1258 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroshi Gyobu: "Generation and Targeting of Human Tumor-Specific Tc1 and Th1 cells Transduced with a Lentivirus Containing a Chimeric Immmunoglobulin."Cancer research. 64. 1490-1495 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasushi Cho: "CD4+ and CD8+ T Cells Cooperate to Improve Prognosis of Patients with Esophageal Squamous Cell Carcinoma"Cancer research. 63. 1555-1559 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toru Nakamura: "Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissection."Oncogene. 23. 2385-2400 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoko Takahashi: "Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system."Experimental Cell Research. 293. 144-153 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashida H: "Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool"Br J Cancer. 90. 1252-1258 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakakubo Y: "The prognostic significance of RCAS1 expression in squamous cell carcinoma of the oesophagus"Cancer Letters. 177. 101-105 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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