| Project/Area Number |
14370374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
OKOUCHI Nobuhiro University of Tsukuba, Institute of Clinical Medicine, Professor, 臨床医学系, 教授 (40213673)
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| Co-Investigator(Kenkyū-buntansha) |
YUZAWA Kenji University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (10240160)
SATO Eimei Tohoku University, Graduate School of Agricultural Science, Professor, 農学部, 教授 (80093243)
YASUE Hiroshi National Institute of Agrobiological Sciences, Seniority Researcher, 上席研究官 (80113497)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2003: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2002: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | procine / xenoantigen / α1-,3galactosyltransferase / antisense / RNA / 遺伝子導入 / ブタ細胞 |
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| Research Abstract |
Objective: In organ transplantation, shortage of donor is a big problem all over the -world. For the solution of the organ shortage xenotransplantation is considered. In pig-to-human xenotransplantation, the hyper-acute rejection triggered by human xenoreactive natural antibody and complement is a big barrier we should overcome. In this study we tried to make knock-out pig in which natural antigen (a 1,3-galactose) and studied whether the antisense RNA vector for a 1,3 -galactosyltransferase could decrease the major xenoantigen in order to control the hyperacute rejection. Methods: We created both knock out vector of 1,3-galactosyltransferase in exon 9 and the antisense of alpha-i, 3-galactosyltransferase RNA vector. And we transfected to a pig stem like cell and analyzed the porcine xenoantigen of the cells. Result: Knock out vector was tranfected into the stem like cells but a 1,3-galactose could not be deceased. Although the antisense was transient expression, it was able to decrease the xenoantigen of a pig stem like cell.. Conclusion: The method that expressed an antisense RNA by transfection and prevented translation of alpha-1, 3-galactosyltransferase protein has a possibility that would be one of the means to control hyperacute rejection.
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