| Project/Area Number |
14370381
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
OMURA Kenji Kanazawa University Hospital, Lecturer, 医学部附属病院, 講師 (30194301)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Kazuyuki Kanazawa University, graduate School of Medicine, Associate, 大学院・医学系研究科, 助手 (00293358)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2003: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | colorectal cancer / chromosome 18 / loss of heterozygosity / allelic loss / microsatellite marker / thymidylate synthase / polymerase chain reaction / gene polymorphism / complementary DNA / loss of heterogeneity / 癌抑制遺伝子 |
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| Research Abstract |
We examined the loss of heterozygosity of chromosome 18p in 177 normal colonic mucosa and corresponding colorectal cancer tissue by PCR using microsatellite markers D18S59,D18S476,D18S481,D18S52,D18S452 and D18S57. Furthermore, the number of the tandem repeat sequences existing in the non-translational region of the TS mRNA was determined by the PCR under the conditions described elsewhere. Of 172 normal colonic mucosa, 90 showed the TS genotype of double repeat(2R)/triple repeat(3R). The 18p allelic loss, including TS locus, was observed 58 samples corresponding to the 90 normal mucosa of 2R/3R TS genotype. The frequency of the loss of TS locus was 30%(12/40) for 2R/loss, and 48%(19/40) for 3R/loss. The frequency of allelic loss including TS locus is comparable for the cancer tissues with 2R/2R or 3R/3R. Consequently, the TS genotype should not affect the LOH of 18p including TS locus. The extent of the loss of 18p in colorectal cancer tissue estimated using 7 kinds of microsatellite markers was very wide. It was quite difficult to find new gene(s) contributing to the carcinogenesis of colorectal cancer. The allelic loss did not affect the expression of TS mRNA or TS protein, regardless the extent of the loss of 18p. The expression of TS protein was significantly high in the colorectal cancer tissue which had TS gene with 3R. It seemed that the expression of TS mRNA significantly contributed to the high expression of TS prortein.
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