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Clinical and basic research for application of liver transplantation for the treatment of hepatocellular carcinoma

Research Project

Project/Area Number 14370389
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOsaka University

Principal Investigator

NAKAMORI Shoji  Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (70294080)

Co-Investigator(Kenkyū-buntansha) NAGANO Hiroaki  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (10294050)
DONO Kezo  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60283769)
加藤 友朗  大阪大学, 医学系研究科, 助手 (90324772)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsHepatocellular carcinoma / Liver transplantation / Selection criteria / Recurrence / Adjuvant therapy / Prognosis / Prevention / Prognostic factor / 癌細胞検出
Research Abstract

1)Clinical trial of liver transplantation for patients with hepatocellular carcinoma.
Total 38 patients have successfully undergone liver transplantation until January, 2004. Among 38 patients, 14 patients were suffered from hepatocellular carcinoma. Although all of the 14 hepatocellular carcinoma patients are still alive for two to 26 months after surgery, two patients have recurrence at bone or lung. Ten of the 14 patients were not considered to be fulfilled with Milan criteria that is used to be a inclusion criteria for application of liver transplantation for patients with hepatocellular carcinoma.
2)Detection of hepatocellular carcinoma cells in peripheral blood in patients underwent liver transplantation.
Using our established method for detection of hepatocellular carcinoma cells in peripheral blood with real time PCR (Int J Oncol 18 : 527-532, 2001), four patients of 14 patients who underwent liver transplantation for hepatocellular carcinoma were positive. Two of four patients wh … More o were positive for hepatocellular carcinoma cells in peripheral blood recurred. These results suggested that detection of hepatocellular carcinoma cells by real time PCR should be potentially useful for stratifying the patients who are at high risk for recurrence and consequently would have benefit from liver transplantation.
3)Safety and feasibility of adjuvant therapy for the patients who underwent liver transplantation.
To prevent implantation of cancer cell during transplantation, Adriamycine was intravenously administered after removal of recipient liver to the 10 patients who underwent transplantation for hepatocellular carcinoma. Any adverse effects due to adriamycine were not observed perioperative time. Safety and feasibility of our adjuvant therapy was confirmed.
4)Prognostic factors of hepatocellular carcinoma.
To understand prognostic factors of hepatocellular carcinoma is essential for stratify the patients who could enjoy the benefit from liver transplantation. We identified that expression of CDC25A phosphatase, Akt2, and LOH of p73 gene were correlated with prognosis of the patients with hepatocellular carcinoma.
5)Establishment of cDNA array system for expression analysis.
To investigate genetic changes in liver after transplantation may be useful for understanding. To analyze the expression profile, we established ATAC-PCR system. This system is useful for molecular features of non-B, non-C hepatocellular carcinoma. This result suggested that our system should be applicable for analyzing genetic difference in the liver after transplantation which Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Sakon M., Nakamori S.et al.: "Intrahepatic recurrences of hepatocellular carcinoma after hepatectomy. Analysis based on tumor hemodynamics."Arch Surg. 137. 94-99 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakon M., Nakamori S.et al.: "Combined intraarterial 5-Fluorouracil and Subcutaneous interferon-α therapy advanced hepatocellular carcinoma with tumor thrombi in the major portal branches"Cancer. 94(2). 435-442 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi H., Nakamori S.et al.: "A Successful resection and long-term sruvival of a patient with intrahepatic recurrences of combined hepatocellular-cholangiocarcinoma : report of a case"Surg Today. 32. 742-746 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 辻江正徳, 中森正二 他: "Neoadjuvant Therapy肝・膵"癌治療と宿主. 14(4). 39-46 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 太田英夫, 中森正二 他: "経非経肝エタノール注入によりグリソン鞘内に局所再発を来した肝細胞癌の1例"癌と化学療法. 29(12). 2441-2424 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto S., Nakamori S.et al.: "Elevated expression of valosin-containing protein (p97) in hepatocellular carcinoma Is correlated with increased incidence of tumor recurrence."J Clin Oncol. 21. 447-452 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi S., Nakamori S.et al.: "Gene transfer into the liver by plasmid injection into the portal vein combined with electroporation."J Gene Med. 5(3). 201-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kondo M., Nakamori S.et al.: "The effect of beta-blocker on intractable ascites in cirrhotic patients undergoing hepatectomy for hepatocellular carcinoma."Hepato-Gastroenterology. 50. 504-506 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyamoto A., Nakamori S.et al.: "Living-related liver transplantation with renoportal anastomosis for a patient with large spontaneous splenorenal collateral."Transplantation. 79(9). 1596-1598 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi S., Nakamori S.et al.: "Electroporation-mediated ex vivo gene transfer into graft not requiring injection pressure in orthotopic liver transplantation."J Gene Med. 5(6). 510-517 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu X., Nakamori S.et al.: "Overexpression of CDC25A phosphatase is associated with hypergrowth activity and poor prognosis of human hepatocellular carcinomas."Clinical Cancer Research. 9. 1764-1772 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu J., Nakamori S.et al.: "Noninvasive quantitative measurement of tissue blood flow in hepatocellular carcinoma using xenon-enhanced computed tomography."Dig Dis Sci. 48(8). 1510-1516 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okami J., Nakamori S.et al.: "Patterns of regional lymph node involvement in intrahepatic cholangiocarcinoma of the left lobe."J Gastrointest Surg. 7(7). 850-856 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurokawa Y., Nakamori S, et al.: "Molecular features of non-B, non-C hepatocellular carcinoma : a PCR-array gene expression profiling study."J Hepatol. 39(6). 1004-1012 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Damdinsuren B., Nakamori S.et al.: "Interferon-beta is more potent than interferon-alpha in inhibition of human hepatocellular carcinoma cell growth when used alone and in combination with anticancer drugs."Ann Surg Oncol. 10. 1184-1190 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto O., Nakamori S., et al.: "Diagnosis of intrahepatic metastasis and multicentric carcinogenesis by microsatellite loss of heterozygosity in patients with multiple and recurrent hepatocellular carcinomas."J Hepatol. 39(2). 215-221 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto H., Nakamori S., et al.: "JTE-522, a cyclooxygenase-2 inhibitor, is an effective chemopreventive agent against rat experimental liver fibrosis."Gastroenterology. 125(2). 556-571 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu X., Nakamori S., et al.: "Akt2 expression correlates with prognosis of humann hepatocellular carcinoma."Oncol Rep. 11(1). 25-32 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki T., Nakamori S., et al.: "Clinical value of alterations in p73 gene, related to P53 at lp36, in human hepatocellular carcinoma."Int J Oncol. 24(2). 441-446 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 永野浩昭, 中森正二 他: "多様化する肝癌治療法.2.外科手術の展開"Frontiers in Gastroenterolgy. 8(2). 37-44 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 太田英夫, 中森正二 他: "5-FU, CDDP, Adriamycinの3剤併用動注化学療法によりCRが得られた進行肝細胞癌(Vp4,Vv3,IM3)の一例"癌と化学療法. 30(11). 1673-1677 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中村将人, 中森正二 他: "TAE抵抗性の多発肝細胞癌に対して肝右葉切除術後長期予後を得た1例"癌と化学療法. 30(11). 1717-1721 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 左近賢人, 中森正二 他: "インターフェロン併用動注化学療法"肝胆膵. 46(5). 583-589 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakon M., Nakamori S.et al.: "Intrahepatic recurrences of hepatocellular carcinoma after hepatectomy : analysis based on tumor hemodynamics."Arch.Surg.. 137. 94-99 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakon M., Nakamori S.et al.: "Combined intraarterial fluorouracil and subcutaneous interferon-alpha therapy for advanced hepatocellular carcinoma with tumor thrombi in the major portal branches."Cancer. 94(2). 45-442 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi H., Nakamori S.et al.: "A successful resection and long-term survival of a patient with intrahepatic recurrence of combined hepatoceUular-cholangiocarcinoma : Report of a case."Surg.Today. 32. 742-746 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto S., Nakamori S.et al.: "Elevated expression of valosin-containing protein (p97) in hepatocellular carcinoma is correlated with increased incidence of tumor recurrence"J.Clon Oncol.. 21. 447-452 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi S., Nakamori S.et al.: "Gene transfer into the liver by plasmid injection into the portal vein combined with electroporation."J.Gene Med.. 5(3). 201-208 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kondo M., Nakamori S.et al.: "The effect of beta-blocker on intractable ascites in cirrhotic patients undergoing hepatectomy for hepatocellular carcinoma."Hepat-Gastroenterol. 50. 504-506 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyamoto A., Nakamori S.et al.: "Living related liver transplantation with renoportal anastomosis for a patient with large spontaneous splenorenal collateral."Transplantation. 75(9). 1596-1598 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi S., Nakamori S.et al.: "Electroporation-mediated ex vivo gene transfer into graft not requiring injection pressure in orthotopic liver transplantation."J.Gene Med.. 5(6). 510-517 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu X., Nakamori S.et al.: "Overexpression of CDC25A phosphatase is associated with hypergrowth activity and poor prognosis of human hepatocellular carcinomas."Clin.Cancer Res.. 9(5). 1764-1772 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto 0., Nakamori S.et al.: "Diagnosis of intrahepatic metastasis and multicentric carcinogenesis by microsatellite loss of heterozygosity in patients with multiple and recurrent hepatocellular carcinomas"J.Hepatol. 39(2). 215-221 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto H., Nakamori S.et al.: "JTE-522, a cyclooxygenase-2 inhibitor, is effective chemopreventive agent against rat experimental liver fibrosis."Gastroenterology. 125(2). 556-571 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu J., Nakamori S.et al.: "Noninvasive quantitative measurement of tissue blood flow in hepatocellular carcinoma using xenon-enhanced computed tomography."Dig.Dis.Sci.. 48(8). 1510-1516 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okami J., Nakamori S.et al.: "Paterns of regional lymph node involvement in intrahepatic cholangiocarcinoma of the left lobe."J.Gastrointest Surg.. 7(7). 850-856 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurokawa Y., Nakamori S.et al.: "Molecular features of non-B, non-C hepatocellular carcinoma : a PCR-array gene expression profiling study."J.Hepatol.. 39(6). 1004-1012 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Damdinsuren B., Nakamori S.et al.: "Interferorrbeta is more potent than interferon-alpha in inhibition of human hepatocellular carcinoma cell growth when used alone and in combination with anti-cancer dtugs."Ann.Surg.Oncol.. 10. 1184-1190 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu X., Nakamori S.et al.: "Akt2 expression correlates with prognosis of human hepatocellular carcinoma."Oncol.Rep.. 11(1). 25-32 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki T., Nakamori S.et al.: "Clinical value of alterations in p73 gene, related to p53 at 1p36, in human hepatocellular carcinoma."Int J Oncol. 24(2). 441-446 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurokawa Y, Nakamori S, et al.: "Molecular-based prediction of early recurrence in hepatocellular carcinoma."J Hepatol. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Ogawa M, Nakamori S, et al.: "Hepatic expression of ANG2 RNA in metastatic colorectal cancer."Hepatology. 39(2). 528-539 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Aoki T, Nakamori S, et al.: "Clinical value of alterations in p73 gene, related to p53 at 1p36, in human hepatocellular carcinoma."Int J Oncol. 24(2). 441-446 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xu X, Nakamori S, et al.: "Akt2 expression correlates with prognosis of human hepatocellular carcinoma."Oncol Rep. 11(1). 25-32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Damdinsuren B, Nakamori S, et al.: "Interferon-beta is more potent than interferon-alpha in inhibition of human hepatocellular carcinoma cell growth when used alone and in combination with anti-cancer dtugs."Ann Surg Oncol. 10. 1184-1190 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kurokawa Y, Nakamori S, et al.: "Molecular features of non-B, non-C hepatocellular carcinoma : a PCR-array gene expression profiling study."J Hepatol.. 39(6). 1004-1012 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okami J, Nakamori S, et al.: "Paterns of regional lymph node involvement in intrahepatic cholangioc arcinoma of the left lobe."Gastrointest Surg. 7(7). 850-856 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimizu J, Nakamori S, et al.: "Noninvasive quantitative measurement of tissue blood flow in hepatocellular carcinoma using xenon-enhanced computed tomography."Dig Dis Sci.. 48(8). 1510-1516 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto H, Nakamori S, et al.: "JTE-522, a cyclooxygenase-2 inhibitor, is an effective chemopreventive agent against rat experimental liver fibrosis."Gastroenterology. 125(2). 556-571 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xu X, Nakamori S, et al.: "Overexpression of CDC25A phosphatase is associated with hypergrowth a ctivity and poor prognosis of human hepatocellular carcinomas."Clin Cancer Res.. 9(5). 1764-1772 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto S, Nakamori S, et al.: "Elevated expression of valosin-containing protein (p97) in hepatocellular carcinoma is correlated with increased incidence of tumor recurre"J Clon Oncol. 21. 447-452 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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