| Project/Area Number |
14370431
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAHASHI Jun Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (10270779)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUJI Norio Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (80237312)
SASAI Yoshiki RIKEN, Center for Developmental Biology, Group Director, 理化学研究所・CDB, グループディレクター (20283616)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2003: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | ES cell / Cell Transplantation / Parkinson's disease / Dopaminergic neuron / ドーパミン産生ニューロン / ドーパミン作動性ニューロン |
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| Research Abstract |
Parkinson's disease is a neurodegenerative disorder characterized by loss of midbrain dopaminergic (DA) neurons. ES cells are currently the most promising donor cell source for cell-replacement therapy of Parkinson's disease. We previously described a strong neuralizing activity present on the surface of stromal cells, named SDIA (stromal cell-derived inducing activity). In this study, we generated neurospheres composed of neural progenitors from monkey ES cells, which are capable of producing large numbers of DA neurons. We demonstrated that FGF20, preferentially expressed in the substantia nigra, acted synergistically with FGF2 to increase the number of DA neurons in ES cell-derived neurospheres. We also analyzed the effect of transplantation of DA neurons generated from monkey ES cells into 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys, a primate model for Parkinson's disease. Behavioral studies and functional imaging revealed that the transplanted cells functioned as DA neurons and attenuated MPTP-induced neurological symptoms.
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