| Project/Area Number |
14370432
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
SAITOH Youichi Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 講師 (20252661)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Amami Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (00233776)
YOSHIMINE Toshiki Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (00201046)
EGUCHI Yutaka Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20243206)
小川 智 金沢大学, 医学系研究科, 教授 (90283746)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | gene therapy / HVJ-E / Tet-on system / NL1 / Gene Therapy / Brain protection / HGF / Liposome / Gene therapy |
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| Research Abstract |
1)To create a cell line with controlled and specific secretion of b-endorphin, a new fusion gene was constructed by joining human b-endorphin coding sequence to part of NL1 gene. HEK293 cells carrying Tet-on system transfected with this fusion gene secreted b-endorphin in a dose-dependent manner upon doxycycline(Dox) administration. This cell line was encapsulated and transplanted in the monkey's CSF space, but failed to respond the administered Dox (i.m.). On the other hand, Neuro2A-Tet-on-POMC cells were encapsulated and transplanted in the monkey's CSF space. They secreted ACTH in the monkey's CSF in a dose-dependent manner upon Dox administration. From these results, peptide secretion from encapsulated cells in the CSF space can be controlled through Tet-on system. HEK293 cells may not fit for this experiment.
2)HVJ-E containing the lacZ, GFP-ODN or DsRed was injected into the cisterna magna of rats and monkeys, and they were perfused with 4% paraformaldehyde. Their brains and meninges were removed and 50-μm-thick sections were made. The brains and meninges of both monkeys and rats were examined about the gene transfection of lacZ, GFP-ODN or DsRed. The efficacy of gene transfection was not stable in both animals. It's reason wasnot clear and the physiological examination has not been done.
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