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Study on the mechanisms responsible for the development and growth of dural arteriovenous fistula

Research Project

Project/Area Number 14370444
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionNara Medical University

Principal Investigator

SAKAKI Toshisuke  Nara Medical University, Professor, 医学部, 教授 (20118029)

Co-Investigator(Kenkyū-buntansha) NAKASE Hiroyuki  Nara Medical University, Assistant Professor, 医学部, 講師 (10217739)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥7,100,000 (Direct Cost: ¥7,100,000)
Keywordsdural arteriovenous fistula(DAVF) / vascular endothelial growth factor(VEGF) / cerebral venous hypertension / 脳静脈圧亢進 / 脳静脈左亢進 / 脳静脈亢進
Research Abstract

Although various mechanisms of the development of dural arteriovenous fistula(DAVF) have been proposed, the pathogenesis of these lesions are still unclear. Recent experimental evidence suggested a role of angiogenic growth factors in the genesis of vascular malformations of the central nervous system. To further investigate the pathogenesis of DAVF, we examined the expression of the angiogenic growth factor, vascular endothelial growth factor(VEGF), in rat DAVF model.
<Experiment-1> Male Wistar rats were used. DAVF model (Spetzler) was made, and venous hypertension was induced which were divided into two experimental groups ; immunohistological study group and angiography group. Immunohistological analysis was performed by VEGF antibody 1 week after, and angiography was done 90 days after the surgery. As a result, VEGF expression in the endothelium and the connective tissues of the dura matter in the five rats (33%) and in the neurons in the eleven rats (73%) of the cerebral cortex and the basal ganglia were identified. DAVF formed in 6 among 15 rats (40%). <Experiment-2> Western blot was performed with different periods from 1, 2 and 3 weeks after inducing venous hypertension in the same model. The expression of VEGF was peaked at one week after venous hypertension, and decreased by the order of two and three weeks (1>2>3 week). The expression of immunoreactive VEGF was restricted in the connective tissue and the endothelial layer of the dura matter, cerebral cortical tissue and basal ganglia neurons.
In conclusions, these results strongly suggest a possible contribution of the VEGF system to the growth of DAVF. Venous ischemia by venous hypertension might be responsible for inducing up-regulation of angiogenic factor expression.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All 2005 2004 2003 2002 Other

All Journal Article (12 results) Book (3 results) Publications (13 results)

  • [Journal Article] Vascular endothelial growth factor antagonist reduces brain edema formation and venous infarction.2005

    • Author(s)
      Kimura R.
    • Journal Title

      Stroke (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Observation of arterial and venous thrombus formation by scanning and transmission electron microscopy.2004

    • Author(s)
      Kim Y.J.
    • Journal Title

      Acta Neurochir (Wien) 146・1

      Pages: 45-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Protective effect of C1 esterase inhibitor on reperfusion injury in the rat middle cerebral artery occlusion model.2004

    • Author(s)
      Akita N.
    • Journal Title

      Neurosurgery 52・2

      Pages: 395-401

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Observation of arterial and venous thrombus formation by scanning and transmission electron micro-scopy.2004

    • Author(s)
      Kim Y.J.
    • Journal Title

      Acta Neurochir (Wien) 146(1)

      Pages: 45-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intraventricular transplantation of embryonic stem cell-derived neural stem cells in intracerebral hemorrhage rats.2004

    • Author(s)
      Nonaka M.
    • Journal Title

      Neurol Res 26・3

      Pages: 265-272

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chemical preconditioning prevents paradoxical increase in glutamate release during ischemia by activating ATP-dependent potassium channels in gerbil hippocampus.2003

    • Author(s)
      Nakagawa I.
    • Journal Title

      Experimental Neurology 183・1

      Pages: 180-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Protective effect of C1 esterase inhibitor on reperfusion injury in the rat middle cerebral artery occlusion model.2003

    • Author(s)
      Akita N.
    • Journal Title

      Neurosurgery 52(2)

      Pages: 395-401

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chemical preconditioning prevents paradoxical increase in glutamate release during ischemia by activating ATP-dependent potassium channels in gerbil hippocampus.2003

    • Author(s)
      Nakagawa I.
    • Journal Title

      Experimental Neurology 183(1)

      Pages: 180-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of anti-intercellular adhesion molecule-1 antibody on reperfusion injury induced by late reperfusion in the rat middle cerebral artery occlusion model.2002

    • Author(s)
      Kanemoto Y.
    • Journal Title

      Neurosurgery 51・4

      Pages: 1034-1042

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Novel intraoperative cerebral blood flow monitoring by Laser-Doppler scanner.2002

    • Author(s)
      Nakase H.
    • Journal Title

      Neurol Med Chir (Tokyo) 42・1

      Pages: 1-4

    • NAID

      110002281440

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Effects of anti-intercellular adhesion molecule-1 antibody on reperfusion injury induced by late reperfusion in the rat middle cerebral artery occlusion model.2002

    • Author(s)
      Kanemoto Y.
    • Journal Title

      Neurosurgery 51(4)

      Pages: 1034-1042

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel intraoperative cerebral blood flow monitoring by Laser-Doppler scanner.2002

    • Author(s)
      Nakase H.
    • Journal Title

      Neurol Med Chir (Tokyo) 42(41)

      Pages: 1-4

    • NAID

      110002281440

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 最新脳神経外科手術(三宅悦夫 編集)2003

    • Author(s)
      榊 寿右
    • Total Pages
      35
    • Publisher
      金芳堂,京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] New Neurosurgical Technique(Miyake E.eds.)2003

    • Author(s)
      Sakaki T.
    • Total Pages
      35
    • Publisher
      Kinpodo, Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 最新脳神経外科手術(三宅悦夫編集)2003

    • Author(s)
      榊 寿右
    • Total Pages
      35
    • Publisher
      金芳堂,京都
    • Related Report
      2004 Annual Research Report
  • [Publications] Akita N.: "Protective effect of C1 esterase inhibitor on reperfusion injury in the rat middle cerebral artery occlusion model."Neurosurgery. 52・2. 395-401 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kaido T.: "Brain metastases from urachal carcinoma."J Clin Neuroscience. 10・6. 703-705 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakase S.: "Novel intraoperative cerebral blood flow monitoring by Laser-Doppler scanner"Neurol Med Chir(Tokyo). 42・1. 1-4 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawaguchi S.: "Effect of carotid endarterectomy on the ophthalmic artery"Acta Neurochir(Wien). 144. 427-432 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakases H.: "A scanning technique to measure regional cerebral blood flow and oxyhemoglobin level"Neurosurgery. 48・6. 1335-1342 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okuno S.: "Comparative study of 2,3,5-triphenyltetorazolium chloride (TTC) and hematoxylin-eosin staining for quantification of early ischemic injury in cats."Neurol Res. 23. 657-661 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] 榊 寿右: "標準脳神経外科学(第9版)(山浦晶, 田中隆一, 児玉南海雄編集)"医学書院,東京. 17 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Nakase: "Novel intraoperative cerebral blood flow monitoring by 1aser-Doppler scanner"Neurol Med Chir. 42・1. 1-4 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Nakase: "A scanning technique to measure regional cerebral blood flow and oxyhemoglobin level"Neurosurgery. 48・6. 1335-1342 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Okuno: "Comparative study of 2,3,5-triphenylte trazolium chloride (TTC) and hematoxylin-eosin staining for quantization of early ischemic injury in cats"Neurol Res. 23・6. 657-661 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Nakase: "Clinical study on recurrent intracranial aneurysms"Cereb. Vas. Dis.. 10. 255-260 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Aketa: "Chemical preconditioning with 3-nitropropionic acid in gerbil hippocampal slices : therapeutic window and the participation of adenosine receptor"Exp Neurol. 166. 385-391 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Otsuka: "Effect of age on cerebral venous circulation disturbances in the rat"J Neurosurg. 93. 298-304 (2000)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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