| Project/Area Number |
14370466
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University |
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Principal Investigator |
MATSUDA Seiji Ehime Univ., School of Medicine, Professor, 医学部, 教授 (40173843)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Naoto Ehime Univ., School of Medicine, Professor, 医学部, 教授 (50234836)
SANO Akira Kagoshima Univ., School of Medicine, Professor, 医学部, 教授 (30178800)
MOMINOKI Katsumi Integrated Center for Science, Biological Resources, Lecturer, 総合科学研究支援センター, 講師 (70304615)
SAITO Shouichirou Gifu Univ., Dept. Veterinary Medicine, Associate Professor, 応用生物科学部, 助教授 (60325371)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | spina bifida / failure of neural groove closure / chick embryo / Leg dysfunction / Islet-1 / motor neuron / spinal cord / interneuron |
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| Research Abstract |
We created chicks with spina bifida aperta (SBA) by incising the roof plate of the neural tube of embryos at Hamburger and Hamilton stage 18 or 19. Incision over the length of five and seven somites caused SBA-like malformation in 100% of the hatchlings. The SBO chicks exhibited no symptoms, whereas the SBA chicks exhibited paralysis of a leg muscle and imbalance between an agonist and an antagonist leg muscles. Lesions in these SBA chicks were located in the spinal segments that give rise to motor neurons that innervated the dysfunctional muscles. Histological analysis revealed that there were fewer small spinal interneurons at the site of the lesion in SBA chicks than in the normal chicks and that there was no such difference in the number of the large spinal motor neurons. Leg dysfunctions in this model of SBA may be attributable to the smaller number of interneurons in the spinal segments that contain motor neurons that innervate the dysfunctional muscle. This model may facilitate studies of the pathological mechanisms that lead to leg dysfunctions in SBA chicks. We examined the SBA neural tube in by immuno- histochemical staining with the monoclonal antibody against Islet-1. The number and distribution of the Islet-1 positive motoneurons were determined at the position equivalent in the Lumbar 3rd vertebra and compared between SBA groups and control groups. On E4 and E4.5, the motoneuron number in the SBA group was less than that in the control group. On E6, the difference in the numbers of Islet-1 positive neuron between SBA group and control group was not significant. Moreover, the distribution of the Islet-1 positive cells in SBA group was different from that of control group on E6. These results suggest that the development of motoneuron is delayed in the chick embryos with SBA.
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