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Identification of novel subtype of LPA receptors expressed in terminal Schwann cell

Research Project

Project/Area Number 14370473
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

OKAJIMA Seiichiro  Kyoto Prefectural University of Medicine, Graduate school of medical science, Lecturer, 医学研究科, 講師 (70305580)

Co-Investigator(Kenkyū-buntansha) YAOI Takeshi  Kyoto Prefectural University of Medicine, Graduate school of medical science, Teaching assistant, 医学研究科, 助手 (40311914)
FUSHIKI Shinji  Kyoto Prefectural University of Medicine, Graduate school of medical science, Professor, 医学研究科, 教授 (80150572)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2003: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2002: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsNeuromuscular junction / Schwann cell / Single cell derived total cDNA / Lysophosphatidic acid / Sphingosine-1-phosphate / Degenerate PCR / G-protein coupled receptors / LPA Receptors
Research Abstract

1.Gene analysis of the terminal Schwann cells specific LPA receptor family
Single terminal Schwann cell (TSC) and myelinating Schwann cell (MSC) were isolated from the sciatic nerves of adult Wistar rats. Total RNA from each single cell was purified and total cDNAs from each type of single cells were synthesized and amplified. We have checked the quality of cDNA fractions with S100β as a common marker for Schwann cells. The cDNA fractions containing S100β were used for the following PCR.
We performed PCR using the amplified cDNAs as templates and using specific primers for LPA receptors, to identify members of LPA receptors specifically expressed in TSCs. PCR analysis revealed that 1pA3 and 1pB1 were specifically expressed in TSC.
Using in situ hybridization histochemistry, we examined the expression patterns of members of LPA receptors in soleus muscles of rats. Specific signals for 1pA3 and 1pB1 mRNAs were detected in TSCs.
2.Serching of novel subtype of LPA receptors expressed in terminal Schwann cell
We performed PCR amplification of TSC cDNA using degenerate oligonucleotide primers against amino acid sequences from transmembrane domain. We are now identifying the novel subtype of LPA receptors.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Okajima, S., Hojo, T., Tamai, K., Takai, S., Hirasawa.Y.: "Histological and electrophysiological analysis of the peripheral nerve alloarafis using an immunosuppressive agent"Microscopy Research and Technique. 58. 52-58 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirakawa, H., Okajima, S., Nagaoka, T., Takamatsu, T., Oyamada, M.: "Loss and recovery of the blood-nerve barrier in the rat sciatic nerve after crush injury are associated with expression of intercellular junctional proteins"Experimental Cell Research. 284. 194-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oda, R., Yaoi, T., Okajima, S., Kobashi, H., Kubo, T., Fushiki, S.: "A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display"Biochemical and Biophysical Research Communications. 308. 872-877 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okajima, S., Hojo, T., Tamai, K., Takai, S., Hirasawa, Y.: "Histological and electrophysiological analysis of the peripheral nerve allografts using an immunosuppressive agent"Microscopy Research and Technique. 58. 52-58 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirakawa, H., Okajima, S., Nagaoka, T., Takamatsu, T., Oyamada, M.: "Loss and recovery of the blood-nerve barrier in the rat sciatic nerve after crush injury are associated with expression of intercellular junctional proteins"Experimental Cell Research. 284. 194-208 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oda, R., Yaoi, T., Okajima, S., Kobashi, H., Kubo, T., Fushiki, S.: "A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display"Biochemical and Biophysical Research Communications. 308. 872-877 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okajima, S., Hojo, T., Tamai, K., Takai, S., Hirasawa, Y.: "Histological and electrophysiological analysis of the peripheral nerve allografts using an immunosuppressive agent"Microscopy Research and Technique. 58. 52-58 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirakawa, H., Okajima, S., Nagaoka, T., Takamatsu, T., Oyamada, M.: "Loss and recovery of the blood-nerve barrier in the rat sciatic nerve after crush injury are associated with expression of intercellular junctional proteins"Experimental Cell Research. 284(2). 194-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oda, R., Yaoi, T., Okajima, S., Kobashi, H., Kubo, T., Fushiki, S.: "A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display"Biochemical and Biophysical Research Communications. 308(4). 872-877 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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