| Project/Area Number |
14370482
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | UNIVERSITY OF FUKUI (2004)
福井医科大学 (2002-2003) |
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Principal Investigator |
FUJIHARA Hideyoshi University of Fukui, Anesthesiology, Associate Professor, 医学部, 助教授 (20251803)
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| Co-Investigator(Kenkyū-buntansha) |
FUKADA Satoru University of Fukui, Anesthesiology, Professor, 医学部, 教授 (30116751)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2004: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | Rho-kinase / protein kinase C / intracellular calcium |
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| Research Abstract |
To study the role of PKC in the mechanism of contraction in cerebral artery, using porcine cerebral artery, following experiments were planned :
1)The eleven PKC isofomis were explored by Western blotting method following SDS-PAGE.
2)It is known that one of small GTP binding protein, RhoA, and its downstream effecor, Rho-kinase (Rock II) participate in the calcium sensitization. The presence of these proteins, distribution were examined by Western blotting.
3)Myosin light chain (MLC) and phosphorylated myosin light chain (p-MLC) were subjected to Western Blotting.
4)Two dimentional electrophoresis, silver staining were applied to detect and quantify the p-MLC/MLC ratio.
The presence of the mechasnism of calcium sensitization through Rho-Rho-kinase pathway was indicated.
The other mechanism of calcium sensitization, the PKC -mediated pathway was also indicated due to the presence of the conventional PKCs, α, βI, βII.
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