Therapy of experimental pulmonary hypertension by cyclic GMP related gene transfer
| Project/Area Number |
14370484
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Mie University |
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Principal Investigator |
MARUYAMA Kazuo Mie University, Faculty of Medicine, Professor, 医学部, 教授 (20181828)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Homare Mie University, Hospital, Research Associate, 医学部附属病院, 助手 (90231993)
MURAYAMA Junko Mie University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (50263017)
MITANI Yoshihide Mie University, Hospital, Research Associate, 医学部附属病院, 助手 (60273380)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | ANP / pulmonary hypertension / nitric oxide / hypoxia / NO |
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| Research Abstract |
In all condition causing pulmonary hypertension vascular changes includes new muscularization of normally nonmuscularized peripheral pulmonary arteries, medial hypertrophy of muscular artery and increase in vascular connective tissue protein such as collagen and elastin. Continuous NO inhalation and NO precursor L-arginine prevented the development of hypertensive pulmonary vascular changes in chronic hypoxia. NO increases cyclic-GMP. Atrial natriuretic peptide (ANP) also increase cyclic-GMP. we determined if ANP gene transfer in lung prevent the development of pulmonary vascular changes in chronic hypoxia-induced pulmonary hypertension. ANP gene was transferred by novel-nonviral HVJ (hemagglutinating virus of Japan) envelope vector by intratracheal injection. ANP gene transfer prevented the development of the vascular changes and pulmonary hypertension.
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Report
(3 results)
Research Products
(11 results)
