• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis and detection of the molecules involving hormone refractory prostate cancer by applying Proteomics methodology

Research Project

Project/Area Number 14370517
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

KUBOTA Yoshinobu  Yokohama City Univ., Dept. Urology, Professor, 大学院・医学研究科, 教授 (10106312)

Co-Investigator(Kenkyū-buntansha) UEMURA Hiroji  Yokohama City Univ., Dept. Urology, Associate professor, 附属病院, 助教授 (50244439)
YAO Masahiro  Yokohama City Univ., Dept. Urology, Associate professor, 大学院・医学研究科, 助教授 (00260787)
HIRANO Hisashi  Yokohama City Univ., Kihara institute for biological research, Professor, 木原生物学研究所, 教授 (00275075)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsprostate cancer / hormone refractory cancer / proteomics / angiotensin II receptor / androgen / アンドロゲン受容体 / 前立線癌
Research Abstract

To analyze the key molecules involving progression of prostate cancer to hormone refractory cancer, we have applied proteomics and other molecular biological methodologies in this research project.
We have identified several molecules which expressed diffentially between androgen dependent prostate cancer cells and androgen independent ones in 2D gel analysis by applying anti-body to tyrosin-kinase. We, then, have focused on the Angiotensin II receptor (AT1) molecules among them.
We demonstrated that AT1 receptar is expressed in human prostate cells. Especially, high expression level of AT1 receptor is seen in androgen independent prostate cancer cells. And stimulation by AII induces activation of intracellular signal transduction pathways that lead to the growth of prostate cancer cells. And we showed that candesartan, an angiatensin II receptor (AT1) blocker, inhibits both the growth of prostate cancer calls in vitro and that of tumors produced by prostate cancer cells in nude mice in vivo (Mol cancer Therap. 2003). We also suggested the potential of AT1 receptor blocker as a novel therapy for prostate cancer (J. Urol. 2005).
We think that these study results have made a remarkable contribution to elucidate the mechanisms of hormone refractory prostate cancer as well as to develop a new novel therapy for this disease.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All 2005 2004 2003 2002 Other

All Journal Article (19 results) Publications (9 results)

  • [Journal Article] Angiotensin II blocker decreases serum prostate specific antigen in hormone refractory prostate cancer.2005

    • Author(s)
      Yamagishi T. et al.
    • Journal Title

      The Journal of Urology 173

      Pages: 441-441

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Genistein induces cell growth inhibition in prostate cancer through the suppression of telomerase activity.2005

    • Author(s)
      Ouchi H. et al.
    • Journal Title

      International journal of Urology 12

      Pages: 73-80

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 前立腺癌における新しい分子標的治療2005

    • Author(s)
      上村 博司 他
    • Journal Title

      日本臨床 63・2

      Pages: 339-344

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Angiotensin II blocker decreases serum prostate specific antigen in hormone refractory prostate cancer2005

    • Author(s)
      Yamagishi, T., Uemura, H., Nakaigawa, N., Noguchi, K., Kubota, Y.
    • Journal Title

      The Journal of Urology 173

      Pages: 441-441

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genistein induces cell growth inhibition in prostate cancer through the suppression of telomerase activity2005

    • Author(s)
      Ouchi, H., Ishiguro, H., Ikeda, N., Honi, M., Kubota, Y., Uemura, H.
    • Journal Title

      International Journal of Urology 12

      Pages: 73-80

    • NAID

      10014374307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genistein induces cell growth inhibition in prostate cancer through the suppression of telomerase activity.2005

    • Author(s)
      Ouchi H. et al.
    • Journal Title

      International Journal of Urology 12

      Pages: 73-80

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effectiveness of percent free prostate specific antigen as a predictor of prostate cancer detection on repeat biopsy.2004

    • Author(s)
      Uemura H. et al.
    • Journal Title

      International Journal of Urology 11・7

      Pages: 494-500

    • NAID

      10013441134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effectiveness of percent free prostate specific antigen as a predictor of prostate cancer detection on repeat biopsy2004

    • Author(s)
      Uemura, H., Nakamura, M., Hasumi, H., Sugiura, S., Fujinami, K., Miyoshi, Y., Yao, M., Kubota, Y.
    • Journal Title

      International Journal of Urology 11(7)

      Pages: 494-500

    • NAID

      10013441134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 再燃前立腺癌に対するアンジオテンシンIIレセプターブロッカーの有用性について2004

    • Author(s)
      上村 博司 他
    • Journal Title

      泌尿器外科 17・8

      Pages: 909-913

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Combination Treatment with 1α,25-Dihydroxyvitamin D_3 and 9-cis-Retinoic Acid Directly Inhibits Human Telomerase Reverse Transcriptase Transcription in Prostate Cancer Cells.2003

    • Author(s)
      Ikeda N. et al.
    • Journal Title

      Mol.Cancer Therap. 2

      Pages: 739-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor.2003

    • Author(s)
      Ishiguro H. et al.
    • Journal Title

      Int.J.Cancer 105(1)

      Pages: 26-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combination Treatment with 1α, 25-Dihydroxyvitamin U3 and 9-cis-Retinoic Acid Directly Inhibits Human Telomerase Reverse Transcriptase Transcription in Prostate Cancer Cells2003

    • Author(s)
      Ikeda, N., Uemura, H., Ishiguro, H., Hori, M, Hosaka, M., Kyo, S., Miyamoto, H., Takeda, E., Kubota, Y.
    • Journal Title

      Mol. Cancer Therap. 2

      Pages: 739-746

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 55 kDa nuclear matrix (nmt55) mRNA is expressed in human prostate cancer and is associated with androgen receptor2003

    • Author(s)
      Ishiguro, H., Uemura, H., Fujinami, K., Ikeda, N., Ohta, S., Kubota, Y.
    • Journal Title

      Int. J. Cancer 105(1)

      Pages: 26-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of AR associated protein 55 (ARA55) and androgen receptor in prostate cancer2003

    • Author(s)
      Miyoshi, Y., Ishiguro, H., Uemura, H., Fujinami, K., Miyamoto, H., Miyoshi, Y., Kitamura, H., Kubota, Y.
    • Journal Title

      The Prostate 56

      Pages: 280-286

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Angiotensin II Receptor Blocker Shows Antiproliferative Activity in Prostate Cancer Cells: A Possibility of Tyrosine Kinase Inhibitor of Growth Factor2003

    • Author(s)
      Uemura, H., Ishiguro, H., Nakaigawa, N., Nagashima, Y., Miyoshi, Y., Fujnami, K., Sakaguchi, A., Kubota, Y.
    • Journal Title

      Mol. Cancer Therap. 2

      Pages: 1139-1147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of AR Associated Protein 55 (ARA55) and Androgen Receptor in Prostate Cancer2003

    • Author(s)
      Miyoshi Y. et al.
    • Journal Title

      The Prostate 56

      Pages: 280-286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Liprin-α 2gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP2002

    • Author(s)
      Fujinami, K., Uemura, H., Ishiguro, H., Kubota, Y.
    • Journal Title

      Int. J. Mol. Med. 10

      Pages: 173-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Association of common missense changes in ELAC2 (HPC2) with prostate cancer in a Japanese case-control series2002

    • Author(s)
      Fujiwara, H., Emi, M., Nagai, H., Nishimura, T., Konishi, N., Kubota, Y., Ichikawa, T., Takahashi, S., Shuin, T., Habuchi, T., Qgawa, C., Inoue, K., Skolnick, H.M., Swensen, J., Camp, J.N., Tavtigian, V.S.
    • Journal Title

      J. Hum. Genet. 47

    • NAID

      50000725812

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of Growth of Human Prostate Cancer Xenograft by Transfection of p53 gene: Gene Transfer by Electroporation2002

    • Author(s)
      Mikata, K., Uemura, H., Ohuchi, H., Ohta, S., Nagashima, Y., Kubota, Y.
    • Journal Title

      Mol. Cancer Therap. 1

      Pages: 247-252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Hiroji Uemura et al.: "Angiotensin II receptor blocker shows antiproliferative activity in prostate cancer cells : A possibility of tyrosine kinase inhibitor of growth factor"Molecular Cancer Therapeutics. 2. 1139-1147 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Naoya Ikeda, et al.: "Combination Treatment with 1α, 25-Dihydroxyvitamin D3 and 9-cis-Retinoic acid directly inhibits human telomerase reverse transcriptase transcription in prostate cancer cells"Molecular Cancer Therapeutics. 2. 739-746 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuhide Miyoshi, et al.: "Expression of AR associated protein 55 (ARA55) and androgen receptor in prostate cancer"The Prostate. 56. 280-286 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hitoshi Ishiguro, et al.: "55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor"Int.J.Cancer. 105. 26-32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 窪田 吉信, 三好 康秀: "前立腺がんのジェネティクス"最新医学. 58. 238-251 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 上村 博司, 窪田 吉信: "再燃前立腺癌に対する治療および緩和治療法について"ホルモンと臨床. 51(6). 67-74 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 窪田 吉信 編: "前立腺の病気がわかる本"株式会社 法研. 223 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mikata K. et al.: "Inhibition of growth of human prostate cancer xenograft by transfection of p53 gene : Gene transfer by electroporation"Molecular Cancer Therapeutics. 1. 247-252 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujinami K. et al.: "Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP"Int. J. Mol. Medicine. 10. 173-176 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi