| Project/Area Number |
14370522
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
MURAKAMI Takashi Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20240666)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Yukihiro Tohoku University, Hospital, Associate Professor, 病院・助教授 (10260431)
野田 隆弘 東北大学, 医学部附属病院, 助手 (10343055)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | endometrium / endometriosis / clonality / 子宮平滑筋 / 幹細胞 / クロナリティ / レーザーマイクロダイゼクション |
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| Research Abstract |
Objective : To investigate the clonality of ectopic gland cells in peritoneal endometriosis
Design : Prospective study
Setting : University hospital
Patient(s) : Seven-teen women with surgically diagnosed endometriosis.
Intervention(s) : Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Main Outcome Measure(s) : Clonality analysis using the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay following digestion of the DNA with methylation-sensitiveendonuclease were used.
Result(s) : Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
Conclusion(s) : The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.
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