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Analysis of the interaction between fetal cells and maternal cells in placenta formation using green-mouse embryos

Research Project

Project/Area Number 14370532
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

TASAKA Keiichi  Osaka University, Graduate School of Medicine, Associate professor, 医学系研究科, 助教授 (50155058)

Co-Investigator(Kenkyū-buntansha) SAKATA Masahiro  Osaka University, Hospital, Associate professor, 医学部附属病院, 助教授 (10260639)
OHMICHI Masahide  Osaka University, Graduate School of Medicine, Lecture, 医学系研究科, 講師 (10283764)
TAHARA Masahiro  Osaka University, Graduate School of Medicine, Assistant professor, 医学系研究科, 助手 (00294091)
武田 卓  大阪大学, 医学系研究科, 助手 (20301260)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2002: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsblastcyst / chirion / low-oxygen condition / hypoxia-inducible factor / ovarian cancer / dissemination / Rho / hypoxia-inducible factor 1α / ES細胞 / 卵 / グリーンマウス / マトリゲル
Research Abstract

To determine the relationship between fetal cells and maternal cells in placenta formation, we started this project. Fortunately a laboratory of this institution kindly prepared green mouse for us. We tried to set up 3D culture system. We tried to search optimal conditions for that purpose, which is no successful up to now. Along with them, we performed a lot of related works. During the development of eggs, Y27632, an inhibitor of Rho induced the arrest of development before cavity formation, which was reversible. We determined that Na+/K+exchanger had an critical role in cavity formation. We also clarified that GLUT1 and VEGF were up-regulated under low oxygen conditions in the placental cell lines through HIF-1alpha interaction with a consensus HRE site of the GLUT1 promoter and that up-regulation of RhoA induced by low-oxygen conditions may play an important role in regulation of HIF-1alpha expression in trophoblast cells. We also clarified that invasion and infiltration were blocked by inhibition of Rho system. Alendronate, which blocks Rho system, inhibited adhesion and infiltration in the dissemination morel of ovarian cancer induced by LPA.
As mentioned above, this project has same difficulties in the beginning, however a lot of works were finally published in relation to this project. In the future study on placenta formation, placental tissue from the green mouse should be used.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (22 results)

All 2005 2004 2003 2002 Other

All Journal Article (17 results) Publications (5 results)

  • [Journal Article] Hypoxia up-regulates HIF-1α expression through RhoA activation in trophoblast cells2005

    • Author(s)
      Hayashi, M., et al.
    • Journal Title

      J Clin Endocrinol Metab 90

      Pages: 1712-1719

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Geranylgeranylacetone inhibits lysphosphatidic acid-induced invasion of human ovarian cancer cells in vitro2005

    • Author(s)
      Hashimoto, K., et al.
    • Journal Title

      Cancer 103

      Pages: 1529-1536

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alendronate inhibits intraperitoneal dissemination in in vivo ovarian cancer model2005

    • Author(s)
      Hashimoto, K., et al.
    • Journal Title

      Cancer Res 65

      Pages: 540-545

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alendronate inhibits intraperitoneal dissemination in in vivo ovarian cancer Model2005

    • Author(s)
      Hashimoto, K., et al.
    • Journal Title

      Cancer Res 65

      Pages: 540-545

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hypoxia up-regulates HIF-1α expression through RhoA activation in trophoblast cells2005

    • Author(s)
      Hayashi, M., et al.
    • Journal Title

      J Clin Endocrinol Metab (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Geranylgeranylacetone inhibits lysphosphatidic acid-induced invasion of human ovarian cancer cells in vitro2005

    • Author(s)
      Hashimot, K., et al.
    • Journal Title

      Cancer (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Alendronate inhibits intraperitoneal dissemination in in vivo ovarian cancer model2005

    • Author(s)
      Hashimoto, K., et al.
    • Journal Title

      Cancer Res (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Induction of glucose transporter 1 expression through hypoxia-inducible factor 1α under hypoxic conditions in trophoblast derived cell2004

    • Author(s)
      Hayashi, M., et al.
    • Journal Title

      J Endocrinol 183

      Pages: 145-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Na+/H+ exchanger-3 is involved in mouse blastocyst formation2004

    • Author(s)
      Kawagishi, R., et al.
    • Journal Title

      J Exp Zool 301

      Pages: 767-775

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Rho-kinase is involved in mouse blastocyst cavity formation2004

    • Author(s)
      Kawagishi, R., et al.
    • Journal Title

      Biochem Biophys Res Commun 319

      Pages: 643-648

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Induction of glucose transporter 1 expression through hypoxia-inducible factor 1αunder hypoxic conditions in trophoblast derived cell2004

    • Author(s)
      Hayashi, M., et al.
    • Journal Title

      J Endocrinol 183

      Pages: 145-154

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Etidronate and hormone replacement therapy(HRT) for postmenopausal women with osteoporosis despite HRT2003

    • Author(s)
      Morishige, K., et al.
    • Journal Title

      Arch Gynecol Obstet 268

      Pages: 105-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermal growth factor enhances invasive activity of BeWo choriocarcinoma cells by inducing α2 integrin expression2003

    • Author(s)
      Nakatsuji, Y., et al.
    • Journal Title

      Endocrine J 50

      Pages: 703-714

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rapid changes of flow-mediated dilatation after surgical menopause2003

    • Author(s)
      Ohmichi, M., et al.
    • Journal Title

      Maturitas 44

      Pages: 125-131

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermal growth factor enhances invasive activity of BeVo choriocarcinoma cells by inducing α2 integrin expression2003

    • Author(s)
      Nakatsuji, Y., et al.
    • Journal Title

      Endocrine J 50

      Pages: 703-714

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lysophosphatidic acid induces focal adhesion assembly through Rho/Rho-associated kinase pathway in human ovarian cancer cells2002

    • Author(s)
      Sawada, K., et al.
    • Journal Title

      Gynecol Oncol 87

      Pages: 252-259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alendronate inhibits lysophosphatidic acid-induced migration of human ovarian cancer cells by attenuating the activation of Rho2002

    • Author(s)
      Sawada, K., et al.
    • Journal Title

      Cancer Res 62

      Pages: 6015-6020

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Morishige, K., et al.: "Etidronate and hormone replacement therapy(HRT) for postmenopausal women with osteoporosis despite HRT"Arch Gynecol Obstet. 268. 105-106 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakatsuji, Y., et al.: "Epidermal growth factor enhances invasive activity of BeWo choriocarcinoma cells by inducing α2 integrin expression"Endocrine J. 50. 703-714 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohmichi, M., et al.: "Rapid changes of flow-mediated dilatation after surgical menopause"Maturitas. 44. 125-131 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawada, K., et al.: "Lysophosphatidic acid induces focal adhesion assembly through Rho/Rho-associated kinase pathway in human ovarian cancer cells"Gynecol Oncol. 87(3). 252-259 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sawada, K., et al.: "Alendronate inhibits lysophosphatidic acid-induced migration of human ovarian cancer cells by attenuating the activation of Rho"Cancer Res. 62(21). 6015-6020 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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