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Expression of voltage-gated potassium channels in the inner ear and herediatary deafness : molecular biological analysis

Research Project

Project/Area Number 14370546
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

KIKUCHI Toshihiko  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (70177799)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Naohiro  Tohoku University, Hospital, Research Associate, 医学部附属病院, 助手 (90291260)
KAWASE Tetsuaki  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (50169728)
KOBAYASHI Toshimitsu  Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (80133958)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordsinner ear / herediatary deafness / endocochlear / voltage-gated potassium channel / Kir4.1 / connexin 26 / GLAST / glutamine synthetase / gap junction / pottassium ion / cochlea / sodium channel / Navl.6 / potassium ion / spiral ganglion cell
Research Abstract

1.Inwardly rectifying potassium channel Kir4.1 was expressed in the stria vascularis, cochlear supporting cell and satellite cells surround spiral ganglion cells. Kir4.1 was also found in the outer sulcus cells. These results suggest that inwardly rectifying Kir4.1 potassium channel in the outer sulcus cells may play a important role in the potassium ion recycling mechanism via gap junctions.
2.In the Brn-4-deficient, Na, K-ATPase-like immunoreactivity in the type II fibrocytes of the cochlear lateral wall was remarkably decreased. Connexin 26 was sparsely distributed among the fibrocytes in the mutant cochlear lateral wall. In contrast, Na, K-ATPase and voltage-gated potassium channel KCNQ1 were abundantly expressed in the marginal cells of the stria vascularis in this mutant. These findings suggest that a dysfunction of fibrocytes in the cochlear lateral wall and an interruption of the K+ ion recycling pathway via gap junctions may result in the depression of endocochlear potential and profound hearing loss in human DFN3 non-syndromic deafness.
3.Intense GLAST-like immunoreactivity was found in the type I fibocytes, type II fibrocytes and the suprastrial fibrocytes in the spiral ligament. Glutamine synthetase was abundantly expressed in the type I fibrocytes, the type II fibrocytes, the type III fibrocytes and the suprastrial fibrocytes in the spiral ligament, the basal cells of the stria vascularis. These results suggest that abundant GLAST and glutamine synthetase in the connective tissue cells in the cochlea may function to maintain the perilymphatic glutamate concentration at a low (nontoxic) level.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 菊地俊彦: "内耳におけるギャップ結合システムとカリウムイオンリサイクル機構"Otol Jpn. 13. 88-91 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin Z-H, Kikuchi T, Tanaka K, Kobayashi T: "Expression of glutamate transporter GLAST in the developing mouse cochlea"Tohoku J Exp Med. 200. 137-144 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 須納瀬弘, 高田雄介, 菊地俊彦, 千葉敏彦, 小林俊光: "繰り返す突発難聴の1症例-聴神経腫瘍との鑑別が必要だった小脳橋角部顔面神経鞘腫-"JOHNS. 20. 529-534 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki M, Kikuchi T, Ikeda K: "Endocochlear potential and endolymphatic K^+ changes induced by gap junction blockers"Acta Otolaryngol(Stockh). (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 菊地俊彦: "ラセン靭帯線維細胞とその機能的意義"耳鼻咽喉科展望. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishimaru K, Kikuchi T, Miyabe Y, So E, Yoshida N, Kobayashi T, Takahashi H: "Immunolocalization of voltage-gated potassium channel Kv3.4 subunit in the cochlea"Acta Medika Nagasakiensia. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi T.: "Gap junction systems and potassium ion recycling mechanism in the inner ear"Otol Jpn. 13. 88-91 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin Z-H, Kikuchi T, Tanaka K, Kobayashi T.: "Expression of glutamate transporter GLAST in the developing mouse cochlea"Tohoku J Exp Med. 200. 137-144 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sunose H, Takada K, Kikuchi T, Chiba T, Kobayashi T.: "A case of a facial nerve neurinoma with repeated sudden onsets of hearing loss"JOHNS. 20. 529-534 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki M, Kikuchi T, Ikeda K.: "Endocochlear potential and endolymphatic K^+ changes induced by gap junction blockers"Acta Otolaryngol(Stockh). (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishimaru K, Kikuchi T, Miyabe Y, So E, Yoshida N, Kobayashi T, Takahashi H.: "Immunolocalization of voltage-gated potassium channel Kv3.4 subunit in the cochlea"Acta Medika Nagasakiensia. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin Z-H, Kikuchi T, Tanaka K, Kobayashi T: "Expression of glutamate transporter GLAST in the developing mouse cochlea"Tohoku J Exp Med. 200. 137-144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki M, Kikuchi T, Ikeda K: "Endocochlear potential and endolymphatic K+ changes induced by gap junction blockers. Acta Otolaryngol (Stockh)"Acta Otolaryngol (Stockh). (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kotaro Ishimaru, Toshihiko Kikuchi, Yuka Miyabe, Eigo So, Naohiro Yoshida, Toshimitsu Kobayashi, Haruo Takahashi: "Immunolocalization of voltage-gated potassium channel Kv3.4 subunit in the cochlea"Acta Medika Nagasakiensia. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 菊池俊彦: "内耳におけるギャップ結合システムとカリウムイオンリサイクル機構"Otology Japan. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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