| Project/Area Number |
14370547
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
YUMOTO Eiji Kumamoto University, Graduate School of Medical and Pharmaceutical Sciences, Professor, 大学院・医学薬学研究部, 教授 (40116992)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MASUDA Masako Kumamoto University, University Hospital, Instructor, 医学部附属病院, 助手 (70346998)
SAMEJIMA Yasuhiro Kumamoto University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (50206009)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥8,800,000 (Direct Cost: ¥8,800,000)
|
|---|
| Keywords | Paralysis of the recurrent laryngeal nerve / Nerve growth factor / Ciliary neurotrophic factor / Basic fibroblast growth factor / Regeneration of nerve fibers |
|---|
| Research Abstract |
Three kinds of neurotrophic factors were evaluated in adult rats for their effects on regeneration of the denervated recurrent laryngeal nerve (RLN). After transection of the left RLN, a silicone tube was placed between the nerve stumps, and the gap of the nerves was filled with one of the following solutions ; nerve growth factor (NGF), ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) and phosphate buffer (PBS) for control. Electromyographical and histological evaluations were conducted either 3 weeks or 5 weeks after the manipulation.
Local administration of NGF or bFGF facilitated recovery of the evoked electromyographic response at 3 weeks. CNTF and bFGF increased the total number of regerating nerve fibers at 3 weeks. Recovery of the myelinated nerve fibers were facilitated by application of bFGF. NGF and bFGF were effective in the prevention of the decrease in the size of the motor neuron in the nucleus ambiguus due to transection.
|
