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The mechanism in the diversity and amplification of mucosal immune system -Clinical application of mucosal vaccine-

Research Project

Project/Area Number 14370548
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKagoshima University

Principal Investigator

KURONO Yuichi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (80153427)

Co-Investigator(Kenkyū-buntansha) MATSUNE Shoji  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (00253899)
USHIKAI Masato  Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (00284886)
NISHIMOTO Kengo  Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Research Associate, 医学部・歯学部附属病院, 助手 (50305132)
FUKUIWA Tatsuya  Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (60325785)
AKASHI Mitsuru  Osaka University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (20145460)
宮之原 郁代  鹿児島大学, 医学部・歯学部附属病院, 講師 (40305131)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsHaemophilus influenzae / Streptococcus pneumoniae / Phosphorylcholine / Mucosal vaccine / Intra-nasal vaccination / IgA / IgE / Type I allergy / 粘膜免疫 / 粘間ワクチン / 上気道感染 / IgG / ワクチン / P6 / ナノスフィア / アジュバント / 破傷風菌トキソイド / PC
Research Abstract

1.Immune responses against Haemophilus influenzae(Hi) P6.
Recombinant P6(rP6) and nanosphere were coupled and intra-nasally administered to mice. Although P6-specific serum IgM and IgG levels were increased, secretory IgA was not induced in external secretions, indicating the low antigenesity of rP6 as mucosal antigen.
2.Mucosal immune responses against phosphorylcholine(PC).
PC is contained in all Gram-positive and -negative bacteria. However, the antigenesity of PC is not yet clarified. When PC was intra-nasally administered to mice, systemic as well as mucosal immune responses were induced without using any mucosal adjuvant such as cholera toxin. Moreover, the antibodies induced by PC were cross-reactive with several different strains of Hi and Streptococcus pneumoniae. Those results suggest that PC might be a candidate of mucosal vaccine with broad spectrum.
3.Inhibition of type I allergy by immunization with PC.
Mucosal vaccine frequently induces type I allergy by producing IgE. However, when PC was intra-nasally or intra-peritoneally administered to mice, the production of IgE was significantly reduced. Further, splenic helper T cells isolated from immunized mice with PC produced much less IL-4 compared with the controls. Those findings suggest the efficacy and the safety of PC as a promising mucosal vaccine.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All 2004 2003 2002 Other

All Journal Article (18 results) Publications (7 results)

  • [Journal Article] The role of allergic rhinitis in upper respiratory tract inflammatory diseases.2004

    • Author(s)
      Y.Kurono, S.Matsune et al.
    • Journal Title

      Clin Exp All Rev 4

      Pages: 15-20

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 上気道粘膜免疫の誘導機序2004

    • Author(s)
      黒野祐一
    • Journal Title

      耳鼻免疫アレルギー 22(1)

      Pages: 1-5

    • NAID

      10015422773

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cytokine expression in experimental chronic otitis media with effusion in mice.2004

    • Author(s)
      Kazuhiko Maeda, Yuichi Kurono, et al.
    • Journal Title

      Laryngoscope 114(11)

      Pages: 1967-1972

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of allergic rhinitis in upper respiratory tract inflammatory diseases.2004

    • Author(s)
      Yuichi Kurono, Shoji Matsune, Dong Sun, Masashi Suzuki, Goro Mogi
    • Journal Title

      Clin Exp All Rev 4

      Pages: 15-20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The mechanism in inducing upper respiratory mucosal immune system.2004

    • Author(s)
      Y.Kurono
    • Journal Title

      JJIAO 22(1)

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytokine expression in experimental chronic otitis media with effusion in mice.2004

    • Author(s)
      Kazuhiko Maeda, Takashi Hirano, Issei Ichimiya, Yuichi Kurono, Masashi Suzuki, Goro Mogi
    • Journal Title

      Laryngoscope 114(11)

      Pages: 1967-1972

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of allergic rhinitis in upper respiratory tract inflammatory diseases.2004

    • Author(s)
      Yuichi Kurono, Shoji Matsune et al.
    • Journal Title

      Clin Exp All Rev 4

      Pages: 15-20

    • Related Report
      2004 Annual Research Report
  • [Journal Article] アレルギー性副鼻腔炎の発症機序と確定診断へのアプローチ2004

    • Author(s)
      黒野祐一
    • Journal Title

      耳鼻咽喉科・頭頸部外科 76(5)

      Pages: 99-105

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cytokine expression in experimental chronic otitis media with effusion in mice.2004

    • Author(s)
      Kazuhiko Maeda et al.
    • Journal Title

      Laryngoscope 114

      Pages: 1967-1972

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 鼻茸の発生部位2004

    • Author(s)
      松根彰志, 黒野祐一 他
    • Journal Title

      JOHNS 20

      Pages: 1793-1796

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NALTの形成と機能発現におけるLTとNIKの役割2003

    • Author(s)
      福山 聡, 黒野祐一 他
    • Journal Title

      耳鼻免疫アレルギー 21(1)

      Pages: 15-18

    • NAID

      10012081200

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 上気道の生体防御機構2003

    • Author(s)
      福山 聡, 黒野祐一
    • Journal Title

      JHONS 19(6)

      Pages: 779-782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of nuclear factor-KB in interleukin-8 expression by human adenoidal fibroblasts.2003

    • Author(s)
      Takaki M., Kurono Y.et al.
    • Journal Title

      Laryngoscope 113(8)

      Pages: 1378-1385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of Lymphotoxin and NF-κB inducing kinase for the development and the function of nasopapharymgeal-associated lymphoid tissue2003

    • Author(s)
      Satoshi Fukuyama, Takashi Shikina, Yuichi Kurono, Hiroshi Kiyono
    • Journal Title

      JJIAO 21(1)

      Pages: 15-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mucosal immune system of upper respiratory tract.2003

    • Author(s)
      S.Fukuyama, Y.Kurono
    • Journal Title

      JOHNS 19(6)

      Pages: 779-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of nuclear factor-κB in interleukin-8 expression by human adenoidal fibroblasts.2003

    • Author(s)
      Takaki M, Ushikai M, Deguchi K, Nishimoto K, Matsune S, Kurono Y.
    • Journal Title

      Laryngoscope 13(8)

      Pages: 1378-1385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Initiation of NALT organogenesis is independent of the IL-7R, LTβR, and NIK signaling pathways but requires the Id2 gene and CD3^- CD4^+ CD45^+ cells.2002

    • Author(s)
      Fukuyama S., Kurono Y.et al.
    • Journal Title

      Immunity 17(1)

      Pages: 31-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Initiation of NALT organogenesis is independent of the IL-7R, LTβR, and NIK signaling pathways but requires the Id2 gene and CD3^- CD4^+ CD45^+ cells.2002

    • Author(s)
      Fukuyama S, Hiroi T, Yokota Y, Rennert PD, Yanagita M, Kinoshita N, Terawaki S, Shikina T, Yamamoto M, Kurono Y, Kiyono H
    • Journal Title

      Immunity 17(1)

      Pages: 1-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 福山 聡: "NALTの形成と機能発現におけるLTとNIKの役割"耳鼻免疫アレルギー. 21(1). 15-18 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福山 聡: "上気道の生態防御機構"JHONS. 19(6). 779-782 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takaki M: "The role of nuclear factor - κB in interleukin - 8 expression by human adenoidal fibroblasts."Laryngoscope. 113(8). 1378-1385 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Norimitsu Tanaka: "Immune responses of palatine tonsil against bacterial antigens."International Congress Series. 1257. 141-144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 黒野祐一: "鼻粘膜の免疫生理と病態"日薬理誌. 120. 7-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuyama S., Hiroi T., Kurono Y.: "Initiation of NALT organogenesis is independent of the IL-7R, LTberaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+)cells"Immunity. 17(1). 31-40 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 黒野祐一: "耳鼻咽喉科診療プラクティス9.小児の耳鼻咽喉科診療"文光堂. 2 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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