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Genome-wide microsatellite mapping of multi-factorial ocular diseases.

Research Project

Project/Area Number 14370562
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionYokohama City University

Principal Investigator

MIZUKI Nobuhisa  Yokohama City University, School of Medicine, Professor, 大学院・医学研究科, 教授 (90336579)

Co-Investigator(Kenkyū-buntansha) OHNO Shigeaki  Hokkaido University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (50002382)
NISHIDA Tomomi  Yokohama City University, School of Medicine, Assistant, 医学部, 助手 (90336561)
ITHO Norihiko  Yokohama City University, School of Medicine, Assistant, 医学部, 助手 (80264654)
SAKAKI Yoshiyuki  Tokyo University, School of Medicine, Professor, 医学研究科・理化学研究所, 教授 (10112327)
INOKO Hidetoshi  Tokai University, School of Medicine, Professor, 医学部, 教授 (10101932)
矢吹 和朗  横浜市立大学, 医学部附属市民総合医療センター, 助手 (30347348)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2003: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2002: ¥7,400,000 (Direct Cost: ¥7,400,000)
KeywordsGenome / Microsatellite / SNP / Disease Genes / Mapping / Behcet disease / Cataract / Hypertension
Research Abstract

The completion of the human genome project has led to worldwide interest in screening for genomic polymorphisms associated with disease susceptibility. For this to be accomplished, it is necessary to adopt efficient methods that probe the entire genome systematically, rapidly and accurately by polymorphic markers to identify specific genes with disease associated polymorphisms. Microsatellite (MS) is a repeated sequence consisting of generally two to six nucleotides (repeated unit), scattering throughout the human genome. MS characterized by a high degree of genetic polymorphisms in its number of repeated unit is expected to be an excellent marker in association analysis for evaluating disease susceptibility. The average number of alleles in MS is 10 (much more than that of SNP) and the average length of linkage disequilibrium of MS is 100kb (much longer than that of SNP). The best way to map the disease susceptibility gene, therefore, is first to narrow down candidate disease gene reg … More ions to 100 kb by genome-wide association mapping with MS, and then next to identify disease gene from these 100 kb candidate regions by conventional SNP analysis. We have collected and localized more than 30,000 MS markers in the human genome at 100 kb intervals and started pan genomic (genomewide) MS screening for identification of pathogenic genes of several diseases including hypertension, diabetes mellitus, open angle glaucoma (OAG), steroid glaucoma (steroid susceptibility gene), Behcet's diabetes mellitus, open angle glaucoma (OAG), steroid glaucoma (steroid susceptibility gene), Behcet's disease, high myopia, retinal lattice degeneration, age related macular degeneration (AMD), keratoconus, cataract, and sarcoidosis. Accordingly, we have so far found fifty to a hundred foci that may have hypertension susceptible genes. SNP analyses are under way to finally reach single disease genes. We are continuing MS screening, mapping and identification for the other diseases, demonstrating that our MS based strategy is quite efficient for genomewide mapping of diseases, especially complex or common diseases with multi-factorial basis. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (56 results)

All Other

All Publications (56 results)

  • [Publications] Mizuki N: "Sequenced-based typing of HLA-B^*51 alleles and the significant association of HLA-B^*51O1 and -B^*5108 with Behcet's disease in Greek patients."Tissue Antigens. 59・2. 118-121 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behcet's disease."Tissue Antigens. 60・5. 369-399 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nomura E: "Hyperkaratosis and leukocytosis in transgenic mice carrying MHC class I related gene B (MICB)"Tissue Antigens. 61・4. 300-307 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohgami K: "Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo"Invest Ophthalmol Vis Sci. 44・6. 2694-2701 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohgami K: "Effect of human cationic antimicrobial protein 18 peptide on endotoxin-induced uveitis"Invest Ophthalmol Vis Sci. 44・10. 4412-4418 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cheng Q: "Cdk5/p35 and Rho-kinase mediate Ephrin-A5-induced signaling in retinal ganglion cells"Mol Cell Neurosci. 24・3. 632-645 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zierhut M: "Human genome and diseases : Review, Immunology and functional genomics of Behcet's disease"Cell Mol Life Sci. 60. 1903-1922 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 水木 信久: "ベーチェット病の分子遺伝学"日本の眼科. 74. 15-18 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 西田 朋美: "ベーチェット病の病態"日本の眼科. 74. 19-22 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 水木 信久: "ベーチェット病の分子遺伝学"日本眼科医会報. 47. 58-66 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 水木 信久: "ベーチェット病の分子遺伝学"臨床眼科. 57・8. 1302-1307 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大野 重昭: "内眼炎の基礎と臨床"日眼会誌. 107・7. 405-416 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Recent advance in the pathogenesis of Behcet's disease"Proceedings of the 9th International Conference on Behcet's Disease, (ed.Bang D.). (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 水木 信久: "ベーチェット病の病因と治療"OTKとも. 103. 18-24 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 野村 英一: "NEW MOOK眼科4,眼の自己免疫疾患"金原出版. 187 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 水木 信久: "今日の治療指針2004年度版"医学書院. 1760 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Sequenced-based typing of HLA-B^*51 alleles and the significant association of HLA-B^*5101 and -B^*5108 with Behcet's disease in Greek patients."Tissue Antigens. 59(2). 118-121 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behcet's disease."Tissue Antigens. 60(5). 396-399 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nomura E: "Hyperkaratosis and leukocytosis in transgenic mice carrying MHC class I related gene B (MICB)."Tissue Antigens. 61(4). 300-307 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohgami K: "Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo."Invest Ophthalmol Vis Sci.. 44(6). 2694-2701 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohgami K: "Effect of human cationic antimicrobial protein 18 peptide on endotoxin-induced uveitis."Invest Ophthalmol Vis Sci.. 44(10). 4412-4418 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cheng Q: "Cdk5/p35 and Rho-kinase mediate Ephrin-A5-induced signaling in retinal ganglion cells."Molecular and Cellular Neuroscience. 24(3). 632-645 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zierhut M: "Human genome and diseases : Review, Immunology and functional genomics of Behcet's disease."Cell Mol Life Sci.. 60. 1903-1922 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Molecular genetics of Behcet Disease."J Jpn Ophthalmologists Assoc.. 74. 15-18 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishida T: "Pathology of Behcet disease."J Jpn Ophthalmologists Assoc.. 74. 19-22 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Molecular genetics of Behcet Disease."Japan ophthalmologists association infoemation. 47. 58-66 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Molecular genetics of Behcet's disease."Jpn J Clin Ophthalmol.. 57(8). 1302-1307 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno S: "Basic and clinical features of intraocular inflammation."Nippon Ganka Gakkai Zasshi. 107(7). 405-416 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Recent advance in the pathogenesis of Behcet's disease."Proceedings of the 9th Intemational Conference on Behcet's Disease. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Etiology and Treatment of Behcet disease."OKTtomo. 103. 18-24 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nomura E: "HLA and ocular disease."New Mook Ophthalmology, Autoimmune disease of eye. 4. 44-53 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Progress of immunotherapy."The most up-to-date care of Ophthalmology. 24-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuki N: "Behcet disease"Today's Therapy. 978-979 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nomura: "Hyperkaratosis and leukocytosis in transgenic mice carrying MHC class I related gene B (MICB)"Tissue Antigens. 61・4. 300-307 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohgami K: "Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo"Invest Ophthalmol Vis Sci. 44・6. 2694-2701 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohgami K: "Effect of human cationic antimicrobial protein 18 peptide on endotoxin-induced uveitis"Invest Ophthalmol Vis Sci. 44・10. 4412-4418 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cheng Q: "Cdk5/p35 and Rho-kinase mediate Ephrin-A5-induced signaling in retinal ganglion cells"Mol Cell Neurosci. 24・3. 632-645 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zierhut M: "Human genome and diseases : Review, Immunology and functional genomics of Behcet's disease"Cell Mol Life Sci. 60. 1903-1922 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水木 信久: "ベーチェット病の分子遺伝学"日本の眼科. 74. 15-18 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 西田 朋美: "ベーチェット病の病態"日本の眼科. 74. 19-22 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水木 信久: "ベーチェット病の分子遺伝学"日本眼科医会報. 47. 58-66 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水木 信久: "HLAの分子生物学"臨床眼科. 57・8. 1302-1307 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大野 重昭: "内眼炎の基礎と臨床"日眼会誌. 107・7. 405-416 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizuki N: "Recent advance in the pathogenesis of Behcet's disease"Proceedings of the 9th International Conference on Behcet's Disease, (ed.Bang D.). (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] 水木 信久: "ベーチェット病の病因と治療"OTKとも. 103. 18-24 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 野村 英一: "NEW M00K眼科4,眼の自己免疫疾患"金原出版. 187 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水木 信久: "今日の治療指針2004年度版"医学書院(印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizuki N: "Sequenced-based typing of HLA-B^*51 alleles and the significant association of HLA-B^*5101 and -B*5108 with Behcet's disease in Greek patients"Tissue Antigens. 59・2. 118-121 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuki N: "Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behcet's disease"Tissue Antigens. 60・5. 396-399 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 水木信久: "ベーチェット病の分子遺伝学"日本の眼科. 74. 361-364 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 西田朋美: "ベーチェット病の病態"日本の眼科. 74. 19-22 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nomura E: "Hyperkaratosis and leukocytosis in transgenic mice carrying MHC class I related gene B (MICB)"Tissue Antigens. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizoguchi K: "Discoloration of soft contact lenses caused by eyeliners"CLAOJ. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 水木信久: "OTKとも"56 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuki N: "Proceedings of the 9th International Conference on Behcet's Disease"(in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 水木信久: "免疫学辞典(第2版)"東京化学同人(印刷中).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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