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THE INVOLVEMENT OF BRAIN DERIBED NEUROTROPHIC FACTOR (BDNF) IN DEVELOPMENT<REGENERATION AND MAINTENACE OF THE PERIODONTAL RUFFINI ENDINGS-A STUDY USING BDNF KNOCKOUT MICE-

Research Project

Project/Area Number 14370580
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

MAEDA Takeyasu  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, PROFESSOR, 大学院・医歯学総合研究科, 教授 (40183941)

Co-Investigator(Kenkyū-buntansha) KAWANO Yoshiro  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSISTANT, 大学院・医歯学総合研究科, 助手 (60303129)
AMIZUKA Norio  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSOCIATE PROFESSOR, 大学院・医歯学総合研究科, 助教授 (30242431)
YAMADA Yoshiaki  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, PROFESSOR, 大学院・医歯学総合研究科, 教授 (80115089)
YAMAMURA Kensuke  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSISTANT, 大学院・医歯学総合研究科, 助手 (90272822)
INOUE Kayoko (NOZAWA Kayoko)  NIIGATA UNIVERSITY, GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSISTANT, 大学院・医歯学総合研究科, 助手 (90303130)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥11,300,000 (Direct Cost: ¥11,300,000)
KeywordsRuffini ending BDNF / knockout mice / development / neurotrophic factor / regeneration / image analysis / innervation / 神経支配 / 歯根膜 / 神経終末 / PGP 9.5
Research Abstract

The present study examined the terminal formation and regeneration/development of the periodontal Ruffini endings in the BDNF knockout (homozygous and heterozygous) and wild type mice by immunocytochemical method. The main results obtained in this study are as follows.
1.The periodontal ligament of wild type types ; Ruffini nerve endings with irregular-(type I) and smooth outlines (type II). The periodontal ligament of heterozygous mice had less development and malformation of type I, which fewer arborizations and less expansion of the axon terminals. Image analysis demonstrated a 18% reduction of neural density in wild type than in heterozygous mice.
2.Observation from postnatal 1 to 3 weeks showed significant difference in neural density between homozygous mice and others.
3.An experimental study on the resection of inferior alveolar nerve demonstrated that a depletion of BDNF induced a delay in recovery of the regeneration of the periodontal Ruffini endings, suggesting that BDNF plays crucial roles in the regeneration process of these endings.
In conclusion, our study supported by this grant was clearly able to demonstrate the involvement of BDNF in the regeneration and development, in particular maturation stage, of the periodontal Ruffini endings.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Alkhamrah, B.A.: "The periodontal Ruffini endings in the brain derived neurotrophic factor(BDNF)deficient mice."Arch.Histol.Cytol.. 66・1. 73-81 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harada, F.: "The involvement of brain-derived neurotrophic factor (BDNF) in the regeneration of the periodontal Ruffini endings following transaction of the inferior alveolar nerve."Arch.Histol.Cytol.. 66・2. 183-194 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hoshino, N.: "Involvement of brain-derived neurotrophic factor (BDNF) in the development of periodontal Ruffini endings."Anat.Rec.. 274A. 807-816 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iijima, K.: "Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve."Brain Res.. 979. 146-152 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seo, K.: "Repetitive afferent stimulation propagates excitation in the trigeminal caudalis."Neuroreport. 14. 1321-1325 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Byers, M.R.: "Dental neuroplasticity, neuro-pulpal interactions and nerve regeneration."Microsc.Res.Tech.. 60・5. 503-515 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Alkhamrah, B.A et al.: "The periodontal Ruffini endings in the brain derived neurotrophic factor (BDNF) deficient mice."Arch.Histol.Cytol.. 66(1). 73-81 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Byers, M.R.et al.: "Dental neuroplasticity, neuro-pulpal interactions and nerve regeneration."Microsc.Res.Tech.. 60(5). 503-515 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seo, K.et al.: "Repetitive afferent stimulation propagates excitation in the trigeminal caudalis."Neuroreport. 14. 1321-1325 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hoshino, N.et al.: "Involvement of brain-derived neurotrophic factor (BDNF) in the development of periodontal Ruffini endings."Anat.Rec.. 274A. 707-816 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iijima, K.et al.: "Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve."Brain Res.. 979. 146-152 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeda, T.et al.: "The development and regeneration of the Periodontal Ruffini endings."Niigata J.. 33(2). 1-15 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harada, F.et al.: "The involvement of brain-derived neurotrophic factor (BDNF) in the regeneration of the periodontal Ruffini endings following transaction of the inferior alveolar nerve."Arch.Histol.Cytol.. 66. 183-194 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Alkhamrah, B.A.: "The periodontal Ruffini endings in the brain derived neurotrophic factor (BDNF) deficient mice"Arch.Histol.Cytol.. 66・1. 73-81 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harada, F.: "The involvement of brain-derived neurotrophic factor (BDNF) in the regeneration of the periodontal Ruffini endings following transaction of the inferior alveolar nerve"Arch.Histol.Cytol.. 66・2. 183-194 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hoshino, N.: "Involvement of brain-derived neurotrophic factor (BDNF) in the development of periodontal Ruffini endings"Anat.Rec.. 274A. 807-816 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iijima, K.: "Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve"Brain Res.. 979. 146-152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seo, K.: "Repetitive afferent stimulation propagates excitation in the trigeminal caudalis"Neuroreport. 14. 1321-1325 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Byers, M.R.: "Dental neuroplasticity, neuro-pulpal interactions and nerve regeneration"Microsc.Res.Tech.. 60・5. 503-515 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] B.A.Alkhamrahほか: "The Periodontal Ruffini Endings in the Brain Derived Neurotrophic Factor (BDNF) Deficient Mice"Archives of Histology and Cytology. 66・1. 73-81 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ohshimaほか: "Pulpal Regeneration after Cavity Preparation, With Special Reference to Close Spatio-relationships between Odontoblasts and Immunocompetent Cells"Microscopy Research & Technique. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.T.Byersほか: "Dental Neuroplasticity, Neuro-pulpal Interactions and Nerve Regeneration"Microscopy Research & Technique. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Maedaほか: "Introduction for Specila Issue of "Biology of Tooth Regeneration""Microscopy Research & Technique. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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