| Project/Area Number |
14370587
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
TAKAGI Minoru Nihon University, School of Dentistry, Professor, 歯学部, 教授 (90060061)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Naoko Nihon University, School of Dentistry, Research Assistant, 歯学部, 助手 (80287656)
相馬 美歩 日本大学, 歯学部, 助手 (70328756)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | osteoblasts / osteocytes / differentiation / transcription factors / non-collagenous proteins / gene expression |
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| Research Abstract |
We examined in situ localization of osteoblast/osteocyte factor 45 (OF45) mRNA during bone formation in developing rat mandible. Gene expression of the transcription factors and oseoblast-related proteins such as OF45,dentin matrix protein 1(Dmp1), osteocalcin(OC), bone sialoprotein(BSP) and osteopontin(OPN) was also examined during cell culture not only in primary rat osteoblast-like cells such as differentiating fetal rat calvarial cells(FRCC) and primary rat stromal bone marrow cells(SBMC), but also in two clonal rat osteoblastic cell lines with different stages of differentiation, ROB-C26 and ROS 17/2.8 cells in the presence or absence of a potent, synthetic glucocorticoid dexamethasone(Dex) or bone morphogenetic protein 2(BMP-2). These experimental results obtained the following conclusions. 1.OF45 mRNA is transiently expressed by mature osteoblasts and subsequently expressed by osteocytes throughout ossification in the skeleton and this protein represents to be an important marke
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r of the osteocyte phenotype and most likely participates in regulating osteocyte function. 2.Induction of Runx2 and Osterix mRNAs in FRCC by Dex may be followed by activation of osteoblast marker genes such as OC and BSP mRNAs to produce a bone-specific matrix that subsequently becomes mineralized. Thus, it is likely that Dex may promote osteoblastic differentiation and mineralization of FRCC by inducing the expression of Runx2 and Osterix genes in vitro. 3.Gene expression of OF45 in SBMC is induced strongly by Dex when compared with that of Dmp1,OC, BSP and OPN. 4.The gene expression of the transcription factors (AJ18,Osterix and Dlx5) and the proteins (alkaline phosphatase, OC, BSP and OPN) in ROS 17/2.8 cells is under the control of Dex and is altered associated with mineralization even in the presence or absence of Dex. 5.The expression of AJ18 and Runx2 mRNAs in ROB-C26 cells is under the control of BMP-2 and TGF-β1, both of which exert different effects on AJ18 mRNA expression, but are potent stimulators of Runx2 mRNA expression during osteoblast differentiation. Less
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