• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

investigation of bFGF potential for egenerative therapy of dentin-pulp complex

Research Project

Project/Area Number 14370617
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

SHIMABUKURO Yoshio  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (50231361)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Shinya  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70239490)
NOZAKI Taketakenori  Osaka University, Graduate School of Dentistry, Instructor, 大学院・歯学研究科, 助手 (30263304)
SAHO Teruyuki  Osaka University, Dental Hospital, Instructor, 歯学部附属病院, 助手 (10263295)
HASHIKAWA Tomoko  Osaka University, Graduate School of Dentistry, Instructor, 大学院・歯学研究科, 助手 (00362682)
池澤 一彦  大阪大学, 歯学部附属病院, 講師 (80294114)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥7,500,000 (Direct Cost: ¥7,500,000)
Keywordsdentin-pulp complex / pulp cells / periodontal ligament cells / hyaluronan / regenerative therapy / heparan sulfate / cell migration / basic fibroblast growth factor / 塩基性繊維芽細胞増殖因子 / 創傷治癒 / ヒアルロン酸合成酵素 / シンデカン / ヒト歯髄細胞 / FGF-2 / 覆髄剤 / 修復象牙質 / 石灰化 / ALPase活性
Research Abstract

We examined the effects of FGF-2 on the production of heparan sulfate and hyaluronan by and expression of hyaluronan synthase in human dental pulp cells and periodontal ligament cells. BFGF increased the production of heparan sulfate by human periodontal ligament cells by using enzyme linked immunosorbent assay. Flow cytometric analysis revealed that syndecan-1 expression did not altered but syndecan-2 expression was decreased when human periodontal ligament cells were stimulated with bFGF. In addition, bFGF stimulated the production of hyalurona a by both human dental pulp cells and periodontal ligament cells and the expression of hyaluronan synthase 1 and 2. mRNA. bFGF enhanced cell migration of human dental pulp cells and the bFGF-induced cell migration was inhibited anti-bFGF neutralizing antibody, which suggest that the migratory action is dependent on bFGF. Moreover, the bFGF-increased cell migration of human dental pulp cells was still observed in spite of suppression of cell proliferation with mitomycin C treatment of the cells. These results suggest that the migration of human dental pulp cells induced with bFGF was independent of cell proliferation. Taken together, bFGF may modulate the wound healing with regulating production of extracellular matrix and cell migration. In vivo application of bFGF on the pulp exposure site of the forth anterior teeth of beagle dogs led to acceleration of bard tissue formation compared with control. Therefore, bFGF appears to be useful reagent as regenerative therapy of dentin-pulp complex.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (6 results)

All 2005

All Journal Article (6 results)

  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells2005

    • Author(s)
      Y.Shimabukuro et al.
    • Journal Title

      Journal of Cellular Physiology 203

      Pages: 557-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells2005

    • Author(s)
      Y.Shimabukuro et al.
    • Journal Title

      Journal of Endodontics (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells2005

    • Author(s)
      Y.Shimabukuro, T.Ichikawa, S.Takayama, S.Yamada, M.Takedachi, M.Terakura, T.Hashikawa, S.Murakami
    • Journal Title

      Journal of Cellular Physiology 203

      Pages: 557-563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells2005

    • Author(s)
      Y.Shimabukuro, M.Ueda, T.Ichikawa, Y.Terashima, S.Yamada, Y.Kusumoto, M.Takedachi, M.Terakura, A.Kohyama, T.Hashikawa, S.Murakami
    • Journal Title

      Journal of Endodontics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells2005

    • Author(s)
      Y.Shimabukuro et al.
    • Journal Title

      J of Endodontics (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells2005

    • Author(s)
      Y.Shimabukuro et al.
    • Journal Title

      Journal of Cellular Physiology (In press)

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi