| Project/Area Number |
14370634
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HIR0SHIMA UNIVERSITY |
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Principal Investigator |
OKAZAKI Masayuki Hiroshima Univ., Graduate Sch. of Biomed. Sic. Dept. of Biomaterials Science, Professor, 大学院・医歯薬学総合研究科, 教授 (30107073)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGAWA Yasumasa Hiroshima Univ., Graduate Sch. of Biomed. Sic., Professor, 大学院・医歯薬学総合研究科, 教授 (00127599)
NOMURA Yuuji Hiroshima Univ., Graduate Sch. of Biomed. Sic., Assistant, 大学院・医歯薬学総合研究科, 助手 (80218370)
HIRATA Isao Hiroshima Univ., Graduate Sch. of Biomed. Sic., Assistant, 大学院・医歯薬学総合研究科, 助手 (40346507)
OBARA Masanobu Hiroshima Univ., Graduate Sch. of Biomed. Sic., Associate Professor, 大学院・理学研究科, 助教授 (60132479)
YAMAGUCHI Syunro Osaka Univ., Institute of Scientific and Industrial Res Dept. of Nanomaterials, Assistant, 産業科学研究所, 助手 (40167698)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | apatites / collagen / composites / organic matrix / bone reconstruction & regeneration |
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| Research Abstract |
Recently, scaffold biomaterials have been focused in the tissue engineering field. FGMgCO_3Ap-collagen composite was made by mixing the functionally graded CO_3apatite containing Mg with collagen. Modification of gradational Mg^<2+> ions on the apatite crystals promoted cell adhesion. In addition, as a scaffold material, the FGMgCO_3Ap-collagen composite suggested contributing to bone formation. Mg^<2+> ions may contribute to the bone metabolism of osteoclast and osteoblast action with the integrins at their cell surfaces.
Furthermore, to develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized CO_3Ap was mixed with a neutralized collagen gel and the CO_3Ap-collagen mixtures were lyophilized into sponges in a HAp frame ring with pores. Matrix substances on the pure collagen sponge samples increased with the culture period and the surface of the sponge was almost covered. A sectional view of hematoxylin-eosin staining confirmed that osteoblast cells had well invaded the CO_3Ap-collagen sponge. When these sponge-frame complexes were implanted beneath the periosteum crane of rats, newly created bone was observed toward the inner core of the complex from the surface of the periosteum cranii. These reinforced CO_3Ap-collagen sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic purposes.
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