| Project/Area Number |
14370664
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
TAKAGI Rituo NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Professor, 大学院・医歯学総合研究科, 教授 (20143795)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATA Masaki NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Assistant, 大学院・医歯学総合研究科, 助手 (10242439)
HOSHINA Hideyuki NIIGATA UNIVERSITY, Medical and dental hospital, Lecturer, 医歯学総合病院, 講師 (30173587)
SAKU Takashi NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Professor, 大学院・医歯学総合研究科, 教授 (40145264)
FUJITA Hajime NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Assistant, 大学院・医歯学総合研究科, 助手 (60271805)
IDA Hiroko NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Assistant, 大学院・医歯学総合研究科, 助手 (60293213)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥10,300,000 (Direct Cost: ¥10,300,000)
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| Keywords | oral squamous cell carcinoma / Individual diagnosis / gene expression / real time PCR / MMP / lymph node metastasis / MMP-1 |
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| Research Abstract |
We surveyed the expression of 557 cancer-related genes in 15 cases of histologically well-differentiated oral squamous cell carcinoma(OSCC) by cDNA microarray analysis. U values and results of SAM(significance analysis of microarray) suggested significance of MMP genes and integrin a3 as the biomarker of lymph node metastasis(LNM). We have collected 140 OSCC samples by collaboration of the four institutes. Real time PCR with TaqMan probes are used for the quantification of gene expression levels in OSCC tissues that were incised at biopsy or operation. We have analyzed the 16 genes of MMPs and MMP inhibitors as the possible clinical biomarkers for the cervical LNM. High expression level of MMP-1,MMP-7,MMP-11 and MMP-13 were significantly correlated with existence of the LNM in the 40 cases of tangue OSCC(20 SCC with LNM and 20 without LNM ; Mann-Whitney test, p<0.05). To identify accurate biomarker system for predicting the risk of lymph node metastasis in OSCC, we further analyzed the expression ratios of MMPs to MMP inhibitors(MMPs/TIMP1 or TIMP2 or RECK). As a consequence, only MMP-1 or MMP-7/TIMP1 or TIMP2 exhibited significance, however, the high level of those expression ratio reflected poor prognosis such as multiple LNM, delayed LNM in small T1 cases, and death by uncontrollable tumor including the case of T1-T4. Whereas gene expression analysis by whole tumor tissue involves biases due to the sampling condition, the consistent clinical significance of MMP-1 and MMP-7 indicates the usefulness of these genes as biomarkers for aggressive characteristics of the OSCC.
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