| Project/Area Number |
14370672
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
UEYAMA Yoshiya Yamaguchi University, School of Medicine, Professer, 医学部, 教授 (00168668)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Kunio Kyusyu University, Graduate Scholl of Dentisty, Proffeser, 大学院・歯科研究院, 教授 (90202952)
MANO Takamitsu Yamaguchi University, School of Medicine, Assistant Professer, 医学部, 講師 (80325125)
ITA Masamichi Yamaguchi University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (60176279)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | continuous porous hydroxyapataite ceramic / bone marrow stromal cell / bone regeneration |
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| Research Abstract |
1.The continuous porous hydroxyapatite ceramics (cp-HA) consisted of β-tricalcium phosphate (β-TCP) known as bone displacement materials, and α-tricalcium phosphate (α-TCP) as a thermophile stable phase, and it's characteristics is that the porosity was about 85mm, and the pore diameter was about 0.8mm. Furthermore, we tried making of a cp-HA which lowered pore diameter by the range that osteoblast could infiltrate as much as possible to add to intensity, but pore was clogged up by surface tension of suspension to dip a soft firing polyurethane form to 10% silicon dioxide component apatite suspension by manufacture methods of the cp-HA which we used by this study, and the manufacture of lower than porous diameter 0.8mm cp-HA was impossible. Therefore the experiment was used cp-HA of pore diameter 1.0mm and 0.8mm.
2.In a difference (1.0mm and 0.8mm) of the pore diameter used for the experiment, the difference was not found in ossiferous quantity and bone formation rate. It is thought that this is not different in an invasion of osteoblast to pores in a difference of porous diameter of this level. But a cp-HA whose pore diameter is massive becomes fragile, then it is not used for clinic. In addition, we confirmed that cpHA contacted new bone directly without connective tissue in pore, but cp-HA did not resorb in vivo and was not replaced by bone.
3.In order to make cp-HA included BMSC, it was necessary to mix fibrinogen with BMSC. The difference of quantity of new bone was not confirmed in a condition of cp-HA included BMSC and cp-HA only in 6 months and 12 months, namely a middle long term, in vivo. However, it is though that the experiment in a short term will need in future because the effect of a BMSC component may appear earlier.
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