Gene therapy targeting cell adhesion molecule that regulate metastasis of oral cancer
| Project/Area Number |
14370674
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
HAYASHIDO Yasutaka Hiroshima University, Medical and Dental hospital, Assistant Professor, 医学部・歯学部附属病院, 講師 (70243251)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tetsuji Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00169153)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | oral cancer / squamous cell carcinoma / integrin / cell adhesion / invasion / metastasis / 蛋白分解酵素 |
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| Research Abstract |
Integrins are cell adhesion molecules that play an important role in cell spreading, migration and growth. Altered expression of integrin α v β 3 has been known to contribute to the invasion and melastasis in several malignant tumors. To examine the role of integrin α v subunit in the progression of squamous cell carcinoma (SCC), oral SCC cell line SCCKN was stably transfected with sense or antisense α v cDNA. α v-overexpression in SCCKN cells facilitated the proliferation and MMP-2 binding at the cell surface, and seemed to enhance the ability to invade type I collagen gel. In contrast, the cells transfected with antisense α v cDNA showed the decreased the proliferative activity.
In conclusion, the results indicated that α v plays a role in the progression of SCC by regulating the growth and the proteolytic activity at the cell surface. The inhibition of α v-expression in oral SCC cells might suppress their invasion and metastasis.
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Report
(3 results)
Research Products
(13 results)
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[Publications] Hayashido, Y., Urabe, K., Yoshioka, Y., Kitano, H., Okamoto, T., Matsuya, T.: "Participation of fibroblasts in MMP-2 binding and activation on the surface of oral squamous cell carcinoma cells"Int.J.Oncol.. 22(3). 657-662 (2003)
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[Publications] Hayashido, Y., Nakashima, M., Urabe, K., Yoshioka, H., Yoshioka, Y., Hamana, T., Kitano, H., Koizumi, K., Okamoto, T.: "Role of stromal thrombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cell"Int J Mol Med.. 12(4). 447-452 (2003)
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[Publications] Asada, N., Tanaka, Y., Hayashido, Y., Toratani, S., Kan, M., Kitamoto, M., Nakanishi, T., Kajiyama, G., Chayama, K., Okamoto, T.: "Expression of fibroblast growth factor (FGF) receptor genes in human hepatoma-derived cell lines"In Vitro Cell Dev Biol Anim.. 39(7). 321-328 (2003)
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