| Project/Area Number |
14370693
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
OOSHIMA Takashi OSAKA UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, PROFESSOR, 大学院・歯学研究科, 教授 (80116003)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Taku NAGASAKI UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, DENTISTRYAND AND PHARMACOLOGY, PROFESSOR, 大学院・医歯薬総合研究科, 教授 (00228975)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥9,800,000 (Direct Cost: ¥9,800,000)
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| Keywords | STREPTOCOCCUS MUTANTS / GLUCOSYLTRANSFERASE / GLUCAN BINDING PROTEIN / ISOGENIC MUTANT / SUCROSE DEPENDENT ADHERENCE / GLUCAN / RECOMBINATION PROTEIN / 組み換えタンバク / 歯面付着 / スクロース / 遺伝子欠失変異株 |
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| Research Abstract |
STREPTOCOCCUS MUTANS PRODUCES MULTIPLE GLUCAN-BINDING PROTEINS (Gbps), WHICH PRESUMABLY PROMOTE THE ADHESION OF THE ORGANISM. THREE Gbps, GbpA, GbpB, AND GbpC HAVE BEEN IDENTIFIED AND CHARACTERIZED ; HOWEVER, THE MECHANISM OF ADHESION BETWEEN : GLUCANS AND BACTERIAL CELL SURFACE ARE UNKNOWN. WE USED GTF-AND/OR Gbp-DEFICIENT MUTANTS TO EXAMINE THE ROLE OF GbpC IN THE SUCROSE-DEPENDENT ADHERENCE OF S. MUTANS. THEIR WILD STRAIN MT8148 AND A GbpA-DEFICIENT MUTANT STRAIN A1 INCREASED SUCROSE DEPENDENT CELLULAR ADHERENCE TO A GLASS SURFACE BY THE ADDITION OF rGTFD. HOWEVER, c1, A GbpC-DEFICIENT MUTANT STRAIN, DEMONSTRATED NO CHANGES IN CELLULAR ADHESION WITH ITS ADDITION. FURTHER, THE BINDING OF rGbpC TO THE GLUCAN SYNTHESIZED BY rGTFD WAS SIGNIFICANTLY HIGHER THAN TO THE GLUCAN SYNTHESIZED BY EITHER rGTFC OR rGTFB. THESE RESULTS SUGGEST THAT GbpC PLAYS A CRUCIAL ROLE IN SUCROSE-DEPENDENT ADHERENCE BY BINDING TO THE SOLUBLE GLUCAN SYNTHESIZED BY GTFD.
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