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Establishment of periodontal tissue engineering by FGF-2

Research Project

Project/Area Number 14370709
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

MURAKAMI Shinya  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70239490)

Co-Investigator(Kenkyū-buntansha) SHIMABUKURO Yoshio  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (50231361)
KITAMURA Masahiro  Osaka University, Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (10243247)
YAMADA Satoru  Osaka University, Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (40359849)
SAHO Teruyuki  Osaka University, Dental Hospital, Instructor, 歯学部附属病院, 助手 (10263295)
ASANO Taiji  Kaken Pharmaceutical Co.Ltd., Senior investigator, 総合研究所, 主任研究員
池澤 一彦  大阪大学, 医学部附属病院, 講師 (80294114)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2004: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥8,100,000 (Direct Cost: ¥8,100,000)
Keywordsperiodontal tissue / periodontal tissue regeneration / basic fibroblast growth factor / cytokine therapy / periodontal ligament cell / hyaluronan / PLAP-1 / osteopontin / 塩基性繊維芽細胞増殖因子 / DNAチップ / ヘパラン硫酸 / 歯肉上皮細胞 / グリコサミノグリカン
Research Abstract

We investigated the regulatory effects of basic fibrovlast growth factor(bFGF) on the production of various extracellular matrices which play important roles in the regulation of periodontal tissue regeneration and can be candidate(s) of the carrier material(s) of bFGF-containing regenerative medicine. It was demonstrated that bFGF preferentially induced hyaluronan and heparan sulfate production by human periodontal ligament (HPDL) cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of bFGF-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed that the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS2, both of which contribute to the production of HA with a high molecular mass. In terms of heparan sulfate, the shedding of syndecan-2 of the surfaces of bFGF-treated HPDL cells was observed. The transcriptome analysis of HPDL cells revealed the existence of periodontal ligament associated protein-1 (PLAP-1), novel proteoglycan-like protein. We demonstrated that PLAP-1 negatively regulated the cytodifferentiation of HPDL cells into hard-tissue forming cells and that bFGF downregulated the expression of PLAP-1. In terms of bone-related proteins, bFGF downregulated the expressions of bone sialoprotein, osteonectin and osteocalcin by HPDL cells. Interestingly, however, bFGF clearly enhanced the osteopontin expression at both mRNA and protein levels. The osteopontin was detected in the cytoplasm and the conditioned medium but not on the surfaces of bFGF-treated HPDL cells. Furthermore, it was revealed that ERK1/2 and PKC were involved in the signaling pathway(s) of bFGF-induced osteopontin expression. We are now planning to investigate the efficacy of bFGF plus scaffold material(s) to induce periodontal tissue regeneration by utilizing the severe periodontitis model of beagle dogs.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (20 results)

All 2005 2003 2002 Other

All Journal Article (10 results) Book (6 results) Publications (4 results)

  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells2005

    • Author(s)
      Yoshio Shimabukuro et al.
    • Journal Title

      J.Cell.Physiol. 203

      Pages: 557-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells.2005

    • Author(s)
      Yoshio Shimabukuro et al.
    • Journal Title

      J.Endodontics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis hyaluronan by human Periodontal ligament2005

    • Author(s)
      Yoshio Shimabukuro et al.
    • Journal Title

      J.Cell.Physiol. 203

      Pages: 557-563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells2005

    • Author(s)
      Yoshio Shimabukuro et al.
    • Journal Title

      J.Endodontics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells.2005

    • Author(s)
      Shinya Murakami et al.
    • Journal Title

      J.Cell.Physiol. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells.2005

    • Author(s)
      Yoshio Shimabukuro et al.
    • Journal Title

      J of Endodontics (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 塩基性線維芽細胞増殖因子による歯周組織再生の試み2003

    • Author(s)
      村上 伸也
    • Journal Title

      大阪大学 歯学雑誌 47

      Pages: 75-84

    • NAID

      10011056272

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Periodontal tissue regeneration by bFGF2003

    • Author(s)
      Shinya Murakami
    • Journal Title

      J.Osaka Univ.Dental Society 47

      Pages: 75-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of basic fibroblast growth factor on human gingival epithelial cells2002

    • Author(s)
      Shin-ichi TAKAYAMA et al.
    • Journal Title

      J.Periodontol. 73

      Pages: 1467-1473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of basic fibroblast growth factor on human gingival epithelial cells2002

    • Author(s)
      Shin-ichi Takayama et al.
    • Journal Title

      J.Periodontol. 73

      Pages: 1467-1473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 先端歯科医学の創生(松岡 博)2005

    • Author(s)
      村上 伸也
    • Total Pages
      273
    • Publisher
      大阪大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Origination of Frontier Bio Dentistry (Hiroshi Matsuoka)2005

    • Author(s)
      Shinya Murakami et al.
    • Publisher
      Osaka Univ. Shuppankai
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 再生医療の実際(葛西 文明)2003

    • Author(s)
      村上 伸也
    • Total Pages
      191
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Regenerative Therapy (Bunmei Kasai)2003

    • Author(s)
      Shinya Murakami et al.
    • Publisher
      Yodosha
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 再生医療工学の最先端(島 健太郎 )2002

    • Author(s)
      村上 伸也
    • Total Pages
      265
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Cutting-edge Engineerings for Regenerative (Kentaro Shima)2002

    • Author(s)
      Shinya Murakami et al.
    • Publisher
      CMC Books
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Shin-ichi TAKAYAMA: "Effects of basic fibroblast growth factor on human gingival epithelial cells"J.Periodontol.. 73. 1467-1473 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 村上 伸也: "塩基性線維芽細胞増殖因子による歯周組織再生の試み"大阪大学 歯学雑誌. 2号. 75-84 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shin-ichi TAKAYAMA: "Effects of Basic Fibroblast Growth Factor on Human Gingival Epithelial Cells"J. Periodontol. 73. 1467-1473 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 村上 伸也: "塩基性線維芽細胞増殖因子による歯周組織再生の試み"大阪大学歯学雑誌. 2号(発売予定). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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