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Basic Research for Tissue Engineering of Periodontal Tissue Regeneration : Validity of Enamel Matrix Proteins and Periodontal Ligament Fibroblast Synthesized Proteins.

Research Project

Project/Area Number 14370714
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

KAWASE Toshio  Kanagawa Dental College, Dental Bioengineering, Professor, 歯学部, 教授 (30084784)

Co-Investigator(Kenkyū-buntansha) DEGUCHI Shinnji  Kanagawa Dental College, Periodontology, Professor, 歯学部, 教授 (60121018)
FUJIHARA Tutomu  Kanagawa Dental College, Dental Bioengineering, Assistant Professor, 歯学部, 助教授 (50084778)
NEGISHI Hideyuki  Kanagawa Dental College, Dental Bioengineering, Instructor, 歯学部, 講師 (60121026)
松澤 光洋  神奈川歯科大学, 歯学部, 助手 (60288082)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsEnamel Matrix Proteins / Periodontal Tissue Regeneration / Human Periodontal Ligament Fibroblast(HPLF) / Rat Bone Marrow Cell(RBMC) / Cell Attachment Factors / Scaffold / Expression of Extracellular Matrix Protein / Cell Migration Factor / 歯根膜由来線維芽細胞 / ラット骨髄由来幹細胞 / ECMの遺伝子発現 / DNAチップ解析 / 細胞遊走活性 / 接着分子の遺伝子発現
Research Abstract

Enamel matrix proteins (enamel matrix derivative, Emdogain^<【○!R】>, EMD) have been clinically used regenerate the periodontal tissues. Although it is clamed that EMD can be used to promote new attachment formation around periodontally invoved teeth, the underlying biological mechanism is not understood. We have demonstrated that the secreted proteins of HPLF have a potency for the cell attachment and spreading activity factor (CASF) promoting tissue regeneration.
The present study investigated the effect of EMD on the behavior of rat bone marrow cells(RBMC) in vitro and compared it with that for the conditioned medium protein(CMP) obtained from human periodontal ligament-derived fibroblasts (HPLF), especially their attachment property, alkaline phosphatase(ALPase) activity and the mRNA levels for ALPase, bone sialoprotein(BSP), Osteopontin(OPN), and osteocalcin(OCN). The hydrophobic dishes were coated EMD (25μg/ml) or CMP (25μg/ml) for 24 hrs at 4℃. The dishes were treated with heat inactivated BSA.
RBMCs were obtained from the femora of 7-week-old male rats and cultured in α-MEM containing 10% FCS and 0.25mM ascorbic acid phosphate on hydrophobic culture plates coated with EMD or CMP. RBMCs attached and spread within 3 hours. RBMCs cultured onto EMD coated dishes expressed high ALPase activity and mRNA levels when compared with CMP coated ones. Furthermore, the expression of OPN and OCN mRNAs of RBMCs was significantly enhanced by the presence of EMD. EMD did not play a chemotactic activity. CMP stimulate the migration activity of HPLF.
Our results indicated that RBMCs responded differently to EMD or CMP, and that EMD stimulated osteogenic differentiation of RBMCs. Therefore, HPLF may interacted with EMD coated on dentin surface, proliferate and differentiate. And mesenchymal cells (RBMC) couled be migrated by CMP and differentiated to osteogenic cells.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 2003 2002 Other

All Journal Article (16 results) Publications (5 results)

  • [Journal Article] Bone regeneration : Effect of basic fibroblast/estrogen combination on bone formation in rat bone marrow stromal cels.2005

    • Author(s)
      Ozono S, Kinoshita Y, Negishi H, Kawase T
    • Journal Title

      Bulletin of Kanagawa Dental College 33

      Pages: 25-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cell on PGA/collagen sponge.2005

    • Author(s)
      Fujita H, Kinoshita Y, Ozono S, Negishi H, Kawase T et al.
    • Journal Title

      Tissue Engineering 11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone regeneration : Effect of basic fibroblast/estrogen combination on bone formation in rat bone marrow stromal cells.2005

    • Author(s)
      Ozono S, Kinoshita Y, Negishi H, Kawase T
    • Journal Title

      Bulletin of Kanagawa Dental College 33

      Pages: 25-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal Cell on PGA/collegen sponge.2005

    • Author(s)
      Fujita M, Kinoshita Y, Ozono S, Negishi H, Kawase T et al.
    • Journal Title

      Tissue Engineering 11(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Durability of FTLA Treatment as a Medicament for Dentin Hypersensitivity -Abrasion Resistance and Profiles of Fluoride Release-2005

    • Author(s)
      K.Tomiyama, Y.Mukai, H.Negishi, T.Fujiwara, T.Kawase, et al.
    • Journal Title

      Dental Materials Journal 23(4)

      Pages: 585-592

    • NAID

      110002983810

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Proliferation and Differentiation of Rat Bone Marrow Stromal Cells on PGA/Collagen Sponge2005

    • Author(s)
      M.Fujita, Y.Kinoshita, S.Ozono, H.Negishi, T.Kawase, et al.
    • Journal Title

      Tissue Engineering 11(9-10)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Bone Regeneration : Effect of basic fibroblast Growth factor/estrogen Combinations on bone formation in rat bone marrow stromal cells.2005

    • Author(s)
      S.Ozono, Y.Kinoshita, H.Negishi, T.Kawase
    • Journal Title

      The Bulletin of Kanagawa Dental College 33(1)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] エナメル基質タンパク質によるラット骨髄由来細胞の骨系細胞への分化誘導について2004

    • Author(s)
      大澤 隆, 根岸秀幸, 川瀬俊夫
    • Journal Title

      神奈川歯学 39

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Durability of FTLA treatment as a medicament for dentin hypersensitivity -Abrasion resistance and profiles of fluoride release-.2004

    • Author(s)
      Tomiyama K, Negishi H, Fujihara T, Kawase T, Teranaka T et al.
    • Journal Title

      Dental Materials Journal 23

      Pages: 585-592

    • NAID

      110002983810

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enamel matrix proteins Stimulate Osteogenic differentiation of rat bone marrow derived cells2004

    • Author(s)
      Osawa T, Negishi H, Kawase T
    • Journal Title

      Kanagawashigaku 39

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Durability of FTLA treatment as a medicament for dentin Hypersensitivity-Abrasion resistance and profiles of fluoride release-2004

    • Author(s)
      Tomiyama K, Negishi H, Fujihara T, Kawase T, Teranaka T et al.
    • Journal Title

      Dental Materials Journal 23

      Pages: 585-592

    • NAID

      110002983810

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] エナメル基質タンパク質によるラット骨髄由来細胞の骨系細胞への分化誘導について2004

    • Author(s)
      大澤 隆, 根岸秀幸, 川瀬俊夫
    • Journal Title

      神奈川歯学 39(1)

      Pages: 1-12

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of pubertal treatment with bisphenol A and bis-GMA on sex hormone level in male rats.2003

    • Author(s)
      Saito D, Ozono S, Kawase T, Teranaka T et al.
    • Journal Title

      Environmental Sciences 20

      Pages: 55-61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of pubertal treatment with bisphenol A and bis-GMA on sex hormone level in male rats.2003

    • Author(s)
      Saito D, Ozono S, Kawase T, Teranaka T et al.
    • Journal Title

      Environmental Sciences 20

      Pages: 55-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of enamel matrix derivatives on migration of human periodontal ligament fibroblasts.2002

    • Author(s)
      Deguchi S, Tago K, Yamashita O, Sato S, Kobe Y, Sugaya A, IIjima Y, Kawase T
    • Journal Title

      Bulletin of Kanagawa Dental College 30

      Pages: 27-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of enamel matrix derivative on migration of human Periodontal ligament fibroblasts2002

    • Author(s)
      Deguchi S, Tago K, Yamashita O, Sato S, Kobe Y, Sugaya A, Iijima Y, Kawase T
    • Journal Title

      Bulletin of Kanagawa Dental College 30

      Pages: 27-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 大澤 隆, 根岸秀幸, 川瀬俊夫: "エナメル基質タンパク質によるラット骨髄由来細胞の骨系細胞への分化誘導について"神奈川歯学. 39(1). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Deguchi S, Kawase T et al.: "Effect of enamel matrix protein derivative on migration of human periodontal ligament fibroblasts"Bull. Kanagawa Dent. Col.. 30(1). 27-31 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saito D, Kawase T, Koshika S et al.: "Effects of pubertal treatment with bisphenol A and Bis-GMA on sex hormone level in male rats"Environmental Sciences. 10(1). 55-61 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 室井剛史, 根岸秀幸, 藤原 努, 川瀬俊夫: "歯科用ポリカーボネート中のビスフェノールAの定量と溶出動態について"神奈川歯学. 37(2/3). 76-82 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 富山 潔, 根岸秀幸, 藤原 努, 川瀬俊夫: "象牙質知覚過敏症に対する細管封鎖法"FTLA法"の耐酸性能-水分透過率の測定-"日本歯科保存学会雑誌. 45(4). 688-695 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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