| Project/Area Number |
14370743
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SATOH Masamichi Kyoto.University, Grad. Sch. of Pharmaceu. Sci., Professor, 薬学研究科, 教授 (80025709)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takayuki Kyoto University, Grad. Sch. of Pharmaceu. Sci., Instructor, 薬学研究科, 助手 (30303845)
MINAMI Masabumi Kyoto University, Grad. Sch. of Pharmaceu. Sci., Assistant Professor, 薬学研究科, 助教授 (20243040)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | μ-Opioid receptor / Morphine / Noradrenergic neuron / Transgenic mouse / Knockout mouse / Dopamine-β-hydroxylase / Analgesic effect / Locus coeruleus / 高次脳機能 |
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| Research Abstract |
μ-Opioid receptor, a receptor far morphine, is intensely expressed in the noradrenergic neurons located in the locus coeruleus.. These neurons project into various brain regions including the cerebral cortex, hippocampus and amygdala, and are implicated in memory, emotion and stress responses. However, the molecular bases for the regulation of memory, emotion and stress responses through the opioid system remain to be elucidated. In this study, we generated the genetically modified mouse line in which μ-opioid receptors are expressed only in the not adrenergic neurons by means of the crossbreeding between the μ-opioid receptor KO mouse and the transgenic mouse in which the expression of human μ-opioid receptor gene is regulated by DBH (dopamine β-hydroxyrase) promoter. Using this newly generated mouse line, we showed that the μ-opioid receptors expressed in the noradrenergic neurons are involved in the regulation of stress responses, such as the escape behavior and corticosteroid hormone release induced by the stress. In addition, using the μ-opioid receptor KO mice, we demonstrated that most of the analgesic effect of buprenorphine, one of the analgesics clinically used in Japan, is mediated by μ-opioid receptors. These findings are considered to be useful for the understanding of the roles of μ-opioid receptors in the stress responses and for the improvement of clinical use of buprenorphine.
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