Molecular-genetical approach to mutagenesis

• Project/Area Number: 14380251
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 環境影響評価(含放射線生物学)
• Research Institution: Kyoto University
• Principal Investigator: TODO Takeshi Kyoto University, Radiation Biology Center, Professor, 放射線生物研究センター, 教授 (90163948)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥13,600,000 (Direct Cost: ¥13,600,000); Fiscal Year 2003: ¥4,300,000 (Direct Cost: ¥4,300,000); Fiscal Year 2002: ¥9,300,000 (Direct Cost: ¥9,300,000)
• Keywords: UV-damage / Mutagenesis / TLS DNA polymerase / DNA damage / TLS DNAポリメラーゼ / DNA修復酵素 / 突然変異生成 / ショウジョウバエ / DNA修復 / DNA複製酵素

Research Abstract

Members of the Y-family of DNA polymerases catalyze template-dependent DNA synthesis but share no sequence homology with other known DNA polymerases. Y-family polymerases exhibits high error rates and low processivity when copying normal DNA but are able to synthesize DNA opposite damaged templates. We have found a novel regulatory pathway of Y-family polymerases. The Y-family polymerases are degraded by proteasome pathway in Drosophila cells when they are overexpressed. We have also identified the protein-protein interaction between Y-family polymerases. These findings will clarify the mutagenesis process in the evolutionary viewpoint.

Research Products

• [Journal Article] Identification of cryptochrome DASH from vertebrates (2004)
  • Author(s): H.Daiyasu, T.Ishikawa, K.Kuma, S.Iwai, T.Todo, H.Toh
  • Journal Title: Genes Cells 9(3) Pages: 479-495
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of cryptochrome DASH from vertebrates (2004)
  • Author(s): H.Daiyasu, T.Ishikawa, K.Kuma, S.Iwai, T.Todo, H.Toh
  • Journal Title: Genes Cells. 9(5) Pages: 479-495
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional and structural analyses of Cryptochrome : Vertefrate CRY regions responsible for interaction with the CLOCK : BMAL1 heterodimer and its nuclear localization (2003)
  • Author(s): J.Hirayama, H.Nakamura, T.Ishikawa, Y.Kobayashi, T.Todo
  • Journal Title: J. Biol. Chem 278 Pages: 35620-35628
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins (2003)
  • Author(s): J.Hirayama, I.Fukuda, T.Ishikawa, Y.Kobayashi, T.Todo
  • Journal Title: Nucleic Acids Res 31(5) Pages: 935-943
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional and structural analyses of Cryptochrome : Vertebrate CRY regions responsible for interaction with the CLOCK : BMAL1 heterodimer and its nuclear localization. (2003)
  • Author(s): J.Hirayama, H.Nakamura, T.Ishikawa, Y.Kobayashi, T.Todo
  • Journal Title: J.Biol.Chem. 278 Pages: 35620-35628
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins (2003)
  • Author(s): J.Hirayama, I.Fukuda, T.Ishikawa, Y.Kobayashi, T.Todo
  • Journal Title: Nucleic Acids Res. 31 Pages: 935-943
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Photoreactivation of the Flavin cofactor inxenopus laevis(6-4)photolyase. (2002)
  • Author(s): S.Weber, C.W.M.Kay, H.Mogling, K.Mobius, K.Hitomi, T.Todo
  • Journal Title: Proc. Natl. Acad. Sci. USA 99(3) Pages: 1319-1322
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Zebrafish CRY represses transcription mediated by CLOCK-BMAL heterodimer without inhibiting its binding to DNA (2002)
  • Author(s): T.Ishikawa, J.Hirayama, Y.Kobayashi, T.Todo
  • Journal Title: Genes Cells 7(6) Pages: 1073-1086
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy ion irradiation to diapause eggs (2002)
  • Author(s): E.Kotani, T.Furusawa, S.Nagaoka, K.Nojima, H.Fujii, Y.Sugimura, M.Ichida, E.Suzuki, A.Nagamatsu, T.Todo, M.Ikenaga
  • Journal Title: J Radiat Res 43(3)
  • NAID: 110002328980
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Photoreactivation of the Flavin cofactor in xenopus laevis (6-4) photolyase : Observation of a transient tyrosyl radical by time-resolved electronmagnetic resonance (2002)
  • Author(s): S.Weber, C.W.M.Kay, H.Mogling, K.Mobius, K.Hitomi, T.Todo
  • Journal Title: Proc.Natl.Acad.Sci.USA 99 Pages: 1319-1322
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zebrafish CRY represses transcription mediated by CLOCK-BMAL heterodimer without inhibiting its binding to DNA (2002)
  • Author(s): T.Ishikawa, J.Hirayama, Y.Kobayashi, T.Todo
  • Journal Title: Genes Cells. 7 Pages: 1073-1086
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy ion irradiation to diapause eggs (2002)
  • Author(s): E.Kotani, T.Furusawa, S.Nagaoka, K.Nojima, H.Fujii, Y.Sugimura, M.Ichida, E.Suzuki, A.Nagamatsu, T.Todo, M.Ikenaga
  • Journal Title: J Radiat Res 43 Suppl
  • NAID: 110002328980
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kotani E, Todo T et al.: "Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy ion irradiation to diapause eggs"J.Radiat.Res.. 43. 193-198 (2002)
• [Publications] J.Hirayama, H.Nakamura, I.Ishikawa, Y.Kobayashi, T.Todo: "Functional and Structural Analyses of Cryptochrome : vertebrate CRY regions responsible for interaction with the CLOCK : BMAL1 heterodimer and its nuclear localization"J.Biol.Chem.. 278. 25620-25628 (2003)
• [Publications] Oishi K, Todo T et al.: "Genome-wide expression analysis of mouse liver reveals CLOCK-regulated circadian output genes"J.Biol.Chem.. 278. 41519-41527 (2003)
• [Publications] Veber, C.W.M.Kay, H.Mogling, K.Mobius, K.Hitomi, T.Todo: "Photoreactivation of the Flavin cofactor in xenopus laevis (6-4)Photolyase : Observation of a transient tyrosyl radical by time-resolved electronmagnetic resonance"Proc. Natl. Acad. Sci. USA. 99. 1319-1322 (2002)
• [Publications] T.Ishikawa, J.Hirayama, Y.Kobayashi, T.todo: "Zebrafish CRY represses transcription mediated by CLOCK-BMAL heterodimer with out inhibiting its binding to DNA"Genes Cells. 7. 1073-1086 (2002)
• [Publications] R.Brudler, K.Hitomi, H.Daiyasu, H.Toh, K.Kucho, M.Ishiura, M.Kanehisa, V.Roberts, T.Todo, J.Tainer, E.Getzoff: "Identification of a new cryptochrome class : structure, function and evolution"Mol Cell.. 11. 59-67 (2003)
• [Publications] J.Hirayama, I.Fukuda, T.Ishikawa, Y.Kobayashi, T.Todo: "New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins"Nucleic Acids Res. 31. 935-943 (2003)