| Project/Area Number |
14380251
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Kyoto University |
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Principal Investigator |
TODO Takeshi Kyoto University, Radiation Biology Center, Professor, 放射線生物研究センター, 教授 (90163948)
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| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2003: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2002: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | UV-damage / Mutagenesis / TLS DNA polymerase / DNA damage / TLS DNAポリメラーゼ / DNA修復酵素 / 突然変異生成 / ショウジョウバエ / DNA修復 / DNA複製酵素 |
|---|
| Research Abstract |
Members of the Y-family of DNA polymerases catalyze template-dependent DNA synthesis but share no sequence homology with other known DNA polymerases. Y-family polymerases exhibits high error rates and low processivity when copying normal DNA but are able to synthesize DNA opposite damaged templates. We have found a novel regulatory pathway of Y-family polymerases. The Y-family polymerases are degraded by proteasome pathway in Drosophila cells when they are overexpressed. We have also identified the protein-protein interaction between Y-family polymerases. These findings will clarify the mutagenesis process in the evolutionary viewpoint.
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