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Characterization and control of behavior of microbial community in subsurface soil environment during the in situ bioremediation

Research Project

Project/Area Number 14380270
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境保全
Research InstitutionNagoya University

Principal Investigator

KATAYAMA Arata  Nagoya University, Eco Topia Science Institute, Professor, エコトピア科学研究機構, 教授 (60185808)

Co-Investigator(Kenkyū-buntansha) WATANABE Akira  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (50231098)
MIYAKE Katsuhide  Nagoya University, Eco Topia Science Institute, Associate Professor, エコトピア科学研究機構, 助教授 (90252254)
INOUE Yasushi  Nagoya University, Graduate School of Engineering, Research Associate, 大学院・工学研究科, 助手 (10293648)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥9,000,000 (Direct Cost: ¥9,000,000)
Keywordsin situ bioremediation / natural attenuation / microbial community / subsurface soil / petroleum hydrocarbons / respiratory quinones / microbial transport / free and attached cells / 油の拡散 / キノンプロファイル / 黄色台地土 / 物理性 / 科学性 / 生物性 / キノンプロファイル法 / 油分解活性 / 分解微生物の移動性 / カオリン
Research Abstract

In situ bioremediation has attracted attention as a useful technology for clean up the contaminated soil. For in situ bioremediation, it is essential to understand and control distribution, transportation, and activities of microbial community. However, the behavior of microbial community in subsurface soil has not been well documented. Thus, this study aimed 1)to examine the microbial community structure in subsurface soil several meters below the ground surface, 2)to monitor the changes in microbial community structure during the natural attenuation using a field lysimeter contaminated with petroleum hydrocarbons, and 3)to examine the transport of microbial cells through porous media. This study will provide useful information to the in situ bioremediation
1)Microbial community structure in subsurface soil
Structures of microbial community were examined for upland yellow soil profile, river sand profile, and seashore profile using respiratory quinone profile. The microbial biomass and … More diversity decreased as the increase in depth. However, there are spots with a high biomass and diversity in subsurface soil where carbon content was high. The community structures in subsurface soil were similar among the soil profiles.
2)Changes in microbial community structure during natural attenuation of petroleum hydrocarbons
A field lysimeter under an unsaturated condition was contaminated with petroleum hydrocarbons, and changes in the structure of microbial community in the contaminated soil was monitored using respiratory quinone profiles over 1 year. Petroleum hydrocarbons transported downward in lysimeter by infiltration water. Specific quinone species increased when petroleum hydrocarbon concentration increased to 3000 mg/kg at maximum, indicating propagation of the degrading microorganisms. After petroleum hydrocarbons dissipated, community structure returned to the one before the contamination.
3)Microbial transport through porous media
Transport of microbial cells by infiltration water was examined using a Sho-na-i river soil as a microbial source and glass beads as porous media. A high flow rate of infiltration water resulted in the transport of soil particles, which showed a positive correlation with microbial cells transported, but not in the case of low flow rate. There were differences among the proportions of free and attached bacterial cells between the high and low flow rates of infiltration water. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (30 results)

All 2005 2004 2003 2002 Other

All Journal Article (26 results) Book (1 results) Publications (3 results)

  • [Journal Article] Monitoring microbial community in a subsurface soil contaminated with hydrocarbons by quinone profile2005

    • Author(s)
      Song, D.-J., Katayama, A.
    • Journal Title

      Chemosphere 59

      Pages: 305-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biodegradation kinetics of volatile hydrophobic organic compounds in the cultures with variable fractional volumes2004

    • Author(s)
      Tien, T.H., Katayama, A.
    • Journal Title

      Biotechnology and Bioengineering 85

      Pages: 580-588

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anaerobic Biodegradation of PCP in Japanese Paddy Field Soils2004

    • Author(s)
      Kim, Hyo-Keun et al.
    • Journal Title

      the Korean Journal of Environmental Agriculture 23

      Pages: 138-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 土壌中における有機化学物質の生物有効性(bioavailability)の評価2004

    • Author(s)
      片山新太
    • Journal Title

      第4回有機化学物質研究会「土壌中におけるPOPs残留メカニズムとリスク低減技術-土壌吸着現象の原理と利用-」農薬環境技術研究所

      Pages: 47-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 土壌地下水汚染の浄化技術の開発動向-積極的浄化技術から環境浄化容量の利用へ-2004

    • Author(s)
      片山 新太, 井上 康
    • Journal Title

      農薬環境科学研究 12

      Pages: 57-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biodegradation kinetics of volatile hydrophobic organic compounds in the cultures with variable fractional volumes2004

    • Author(s)
      Tien, T.H., Katayama, A.
    • Journal Title

      Biotechnol.Bioengineer 85

      Pages: 580-588

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anaerobic Biodegradation of PCP in Japanese Paddy Field Soils2004

    • Author(s)
      Kim, H.-K., Inoue, Y., Handa, Y., Yasuta, T., Lee, K.-S., Katayama A.
    • Journal Title

      the Korean Journal of Environmental Agriculture 23

      Pages: 138-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of bioavailability of organic chemicals in soil(In Japanese)2004

    • Author(s)
      Katayama, A.
    • Journal Title

      The fourth seminar on organic chemicals "Persistence mechanisms and Risk-reducing technologies of POPs in soil, Principles of soil sorption and its utilization"(NOGYO KANKYO GIJUTSU KENKYUSHO)

      Pages: 47-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recent trend in R & D of remediation technologies of contaminated soil and groundwater. From active remediation to passive remediation(In Japanese)2004

    • Author(s)
      Katayama, A., Inoue, Y.
    • Journal Title

      NOYAKU KANKYO KAGAKU KENKYU 12

      Pages: 57-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biodegradation kinetics of volatile hydrophobic organic compounds in the cultures with variable fractional volumes2004

    • Author(s)
      Tien, Truong Hong, Katayama, Arata
    • Journal Title

      Biotechnology and Bioengineering 85

      Pages: 580-588

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Application of the Rescue Number to the evaluation of remediation technologies for contaminated ground2004

    • Author(s)
      Inoue, Y., Katayama, A.
    • Journal Title

      Journal of Material Cycles and Waste Management 6

      Pages: 48-57

    • NAID

      10012029982

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anaerobic Biodegradation of PCP in Japanese Paddy Field Soils2004

    • Author(s)
      Hyo-Keun Kim et al.
    • Journal Title

      the Korean Journal of Environmental Agriculture 23

      Pages: 138-141

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 土壌中における有機化学物質の生物有効性(bioavailability)の評価2004

    • Author(s)
      片山新太
    • Journal Title

      第4回有機化学物質研究会「土壌中におけるPOPs残留メカニズムとリスク低減技術-土壌吸着現象の原理と利用-」(農薬環境技術研究所)

      Pages: 47-59

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 土壌地下水汚染の浄化技術の開発動向-積極的浄化技術から環境浄化容量の利用へ-2004

    • Author(s)
      片山新太, 井上 康
    • Journal Title

      農薬環境科学研究 12

      Pages: 57-66

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional characterization of soil microbial community based on utilization pattern of aromatic compounds,2003

    • Author(s)
      Marwati, U., Funasaka, K., Toyota, K., Katayama, A.
    • Journal Title

      Soil Sci.Plant Nutr 49

      Pages: 143-147

    • NAID

      110001717732

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chlorothalonil degradation in soils treated repeatedly with chlorothalonil2003

    • Author(s)
      Ukai, T., T.Itoh, Katayama, A.
    • Journal Title

      J.Pesticide Sci. 28

      Pages: 208-211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Topic 2.7 Role of metabolism in the endocrine-disrupting effects of chemicals in aquatic and terrestrial systems2003

    • Author(s)
      van den Berg, M., Sanderson, T., Kurihala, N., Katayama, A.
    • Journal Title

      Pure and Applied Chemistry 75(11-12)

      Pages: 1917-1932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional characterization of soil microbial community based on utilization pattern of aromatic compounds2003

    • Author(s)
      Marwati, U., Funasaka, K., Toyota, K., Katayama, A.
    • Journal Title

      Soil Sci.Plant Nutr.

      Pages: 143-147

    • NAID

      110001717732

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chlorothalonil degradation in soils treated repeatedly with chlorothalonil2003

    • Author(s)
      Ukai, T., Itoh, T., Katayama, A.
    • Journal Title

      J.Pesticide Sci. 28(2)

      Pages: 208-211

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Topic 2.7 Role of metabolism in the endocrine-disrupting effects of chemicals in aquatic and terrestrial systems2003

    • Author(s)
      van den Berg, M., Sanderson, T., Kurihara, N., Katayama, A.
    • Journal Title

      Pure and Applied Chemistry 75(11-12)

      Pages: 1917-1932

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Numerical simulation of impact by precipitation on bioremediation process of hydrocarbon-contaminated groundwater, IS-OKAYAMA 20032003

    • Author(s)
      Song, D.J., Katayama, A.
    • Journal Title

      Groundwater engineering, -Recent advances-(Edited by Kono, I., Nishigaki, M., Komatsu, M.)

      Pages: 291-298

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in microbial community structure in soils incorporated with the mixture of glucose and peptone with reference to respiratory quinone profile2002

    • Author(s)
      Katayama, A, Hu, H.-y., Nozawa, M., Takahashi, S., Fujie, K.
    • Journal Title

      Soil Sci.and Plant Nutr. 48

      Pages: 841-846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pesticide soil sorption parameters : theory, measurement, uses, limitations and reliability,2002

    • Author(s)
      Wauchope, R.Don, Yeh, S., Linders, Jan B.H.J., Kloskowski, R., Tanaka, K., Rubin, B., Katayama, A., Koedel, W., Gerstl, Z., Lane, M., Unsworth, J.B.
    • Journal Title

      Pest Management Science 58

      Pages: 419-445

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in microbial community structure in soils incorporated with the mixture of glucose and peptone with reference to respiratory quinone profile2002

    • Author(s)
      Katayama, A, Hu, H.-y., Nozawa, M, Takahashi, S., Fujie, K.
    • Journal Title

      Soil Sci.and Plant Nutr. 48

      Pages: 841-846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Unsworth, J.B.Pesticide soil sorption parameters : theory, measurement, uses, limitations and reliability2002

    • Author(s)
      Wauchope, R.Don, Yeh, S., Linders, Jan B.H.J., Kloskowski, R., Tanaka, K., Rubin, B., Katayama, A., Koedel, W., Gerstl, Z., Lane, M.
    • Journal Title

      Pest Management Science 58

      Pages: 419-445

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Monitoring microbial community in a subsurface soil contaminated with hydrocarbons by quinone profile

    • Author(s)
      Song, D.-J., Katayama, A.
    • Journal Title

      Chemosphere (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Book] Groundwater engineering,-recent advances- (Edited by Kono, I., Nishigaki, M., Komatsu, M.)2003

    • Author(s)
      Song, D.J., Katayama, A.
    • Total Pages
      626
    • Publisher
      A.A.Balkema Publishes, Lisse, the Netherlands,(ISBN 90 5809 3859)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Marwati, U., Funasaka, K., Toyota, K., Katayama, A.: "Functional characterization of soil microbial community based on utilization pattern of aromatic compounds"Soil Sci.Plant Nutr. 49. 143-147 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Song, D.J., Katayama, A.: ""Groundwater engineering, -recent advances-" Edited by Kono, I., Nishigaki, M., Komatsu, M."A.A.Balkema Publishers, Lisse, the Netherlands, (ISBN 90 5809 385 9). 626 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katayama, A, 他5名: "Changes in microbial community structure in soils incorporated with the mixture of glucose and peptone with reference to respiratory quinone profile"Soil Science and Plant Nutrition. 48・6. 841-846 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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