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Study on the SHAP-hyaluronoan (HA) complex as a functional entity of HA in the process of inflammation.

Research Project

Project/Area Number 14380298
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionINSTITUTE FOR MOLECULAR SCIENCE OF MEDICINE, AICHI MEDICAL UNIVERSITY

Principal Investigator

KIMATA Koji  AICHI MEDICAL UNIVERSITY, INSTITUTE FOR MOLECULAR SCIENCE OF MEDICINE, PROFESSOR, 分子医学研究所, 教授 (10022641)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsinflammation / hyaluronan / SHAP / trypsin inhibitor / CD44 / cumulas-oocyte complex / ovulation / cell adhesion
Research Abstract

We have shown that HA in the inflammatory tissues with influx of blood usually contain covalently bound proteins that we named SHAP and fount to correspond to the heavy chain subunits of inter a-trypsin inhibitor (ITI) circulating in blood. Therefore, we hypothesized that the SHAP-HA complex is a functional entity of HA involved in inflammation. Here, we demonstrate evidences obtained from the following experiments shown below, and our final goal is to find some ways to regulate inflammations through the study on the regulation of the SNAP-HA complex formation.
1) Electron microscopic observation and biochemical analysis of the complex purified from the synovial fluid obtained from rheumatoid arthritic patients have revealed that the HA of-Mr 1,000,000 in the patient fluid bound about 5 SHAPs and is polymerized via SNAP.
2) SHAP-HA complex-knockout mice obtained by homologous recombination of the genes for molecules involved in the ITI synthesis show not only a marked decrease in the ovu … More lation of the cumulus-oocyte complex (COC) which is caused by the defective formation of the COC, due to the absence of the SNAP-HA complex in the COC matrix, but also the impaired fertilization which is likely due to the abnormality of the zona pellucida judging from the IVF (in vitro fertilization) and ISCI (intra-cytoplasmic spermatozoa injection) experiments.
3) Comparison of cell adhesion and activation of CD44-positive inflammatory lymphocyte cells, HUT78 to the SHAP-HA complex substrata with those to HA alone has revealed that the cell adhesion to the complex is about 100 times more efficient than that to HA and SHAP bound to HA mediates such a high capacity of the HA-CD44 interaction.
4) We have developed the highly sensitive assay method for the activity of the SHAP-HA complex formation and found the presence of the activity in human sera and conditioned media of some liver cells. In collaboration with the Kaketsu Institute Corporation we successfully purified the fraction with the high activity and, based upon the informations of the peptide analysis, have obtained the cDNA of the candidate molecule. We are now on the way to the definitive answer.
5) In order to clarify in vivo role of the SNAP-HA complex in inflammation, we induced type II collagen-induced arthritis and dextran sulfate-induced colitis in the knockout mice, and have found that the disease conditions tented to be significantly reduced, compared to those in wild mice. However, this seemed not the case for ConA-induced hepatitis, which suggests some involvement of the deficient formation of ITI itself in the hepatitis. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (86 results)

All 2005 2004 2003 2002 Other

All Journal Article (53 results) Book (6 results) Patent(Industrial Property Rights) (5 results) Publications (22 results)

  • [Journal Article] マウス初代軟骨細胞におけるbone morphogenetic protein-2のヒアルロン酸合成酵素発現制御2005

    • Author(s)
      澤井崇博, 板野直樹, et al.
    • Journal Title

      愛知医科大学医学会雑誌 33

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hyaluronan (HA) and serum-derived hyaluronan associated protein (SHAP)-HA complex as markers of pre-eclampsia.2005

    • Author(s)
      T.Kishida, H.Yabushita, et al.
    • Journal Title

      愛知医科大学医学会雑誌 33

      Pages: 7-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential regulation by IL-1 β and EGF of expression of three different hyaluronan syntases in oral mucosal epithelial cells and fibroblasts and dermal fibroblasts : Quantitative anylysis using real time RT-PCR.2004

    • Author(s)
      Y.Yamada, N.Itano, et al.
    • Journal Title

      J Invest Dermatol 122

      Pages: 631-639

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Elevated transcript level of hyaluronan synthase 1 gene correlates with poor prognosis of human colon cancer.2004

    • Author(s)
      Y.Yamada, N.Itano, et al.
    • Journal Title

      Clin Exp Metastasis 21

      Pages: 57-63

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.2004

    • Author(s)
      N.Itano, T.Sawai, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 18679-18687

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] In vivo hyaluronan synthesis by expression of mammalian hyaluronan synthase gene in drosophila.2004

    • Author(s)
      S.Takeo, M.Fujise, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 18920-18925

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The globular domains of PGM/versican modulate the proliferation-apoptosis equilibrium and invasive capabilities of tumor cells.2004

    • Author(s)
      S.Cattaruzza, M.Schiappacassi, et al.
    • Journal Title

      FASEB J 18

      Pages: 779-781

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] A novel mechanism for the inhibition of hyaluronan biosynthesis by 4-methylumbelliferone.2004

    • Author(s)
      I.Kakizaki, K.Kojima, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 33281-33289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Inter-α-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex.2004

    • Author(s)
      L.Zhuo, V.C.Hascall, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 38079-38082

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hyaluronan synthase expression in ovarian cancer.2004

    • Author(s)
      H.Yabushita, M.Noguchi, et al.
    • Journal Title

      Oncol Rep 12

      Pages: 739-743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction.2004

    • Author(s)
      M.Yoshida, S.Sai, et al.
    • Journal Title

      Arthritis Res Ther 6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential regulation by IL-1β a and EGF of expression of three different hyaluronan syntases in oral mucosal epithelial cells and fibroblasts and dermal fibroblasts : Quantitative anylysis using real time RT-PCR.2004

    • Author(s)
      Y.Yamada, N.Itano, K.Hata, M.Ueda, K.Kimata.
    • Journal Title

      J Invest Dermatol 122

      Pages: 631-639

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Elevated transcript level of hyaluronan synthase 1 gene correlates with poor prognosis of human colon cancer.2004

    • Author(s)
      Y.Yamada, N.Itano, H.Narimatsu, T.Kudo, K.Morozumi, S.Hirohashi, A.Ochiai, M.Ueda, K.Kimata
    • Journal Title

      Clin Exp Metastasis 21

      Pages: 57-63

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.2004

    • Author(s)
      N.Itano, T.Sawai, F.Atsumi, O.Miyaishi, S.Taniguchi, R.Kannagi, M.Hamaguchi, K.Kimata
    • Journal Title

      J Biol Chem 279

      Pages: 18679-18687

    • NAID

      110003994219

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo hyaluronan synthesis upon expression of the mammalian hyaluronan synthase gene in drosophila.2004

    • Author(s)
      S.Takeo, M.Fujise, T.Akiyama, H.Habuchi, N.Itano, T.Matsuo, T.Aigaki, K.Kimata, H.Nakato
    • Journal Title

      J Biol Chem 279

      Pages: 18920-18925

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The globular domains of PG-M/versican modulate the proliferation-apoptosis equilibrium and invasive capabilities of tumor cells.2004

    • Author(s)
      S.Cattaruzza, M.Schiappacassi, K.Kimata, A.Colombatti, R.Ferris.
    • Journal Title

      FASEB J 18

      Pages: 779-781

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel mechanism for the inhibition of hyaluronan biosynthesis by 4-methylumbelliferone.2004

    • Author(s)
      I.Kakizaki, K.Kojima, K.Takagaki, M.Endo, R.Kannagi, M.Ito, Y.Maruo, H.Sato, T.Yasuda, S.Mita, K.Kimata, N.Itano.
    • Journal Title

      J Biol Chem 279

      Pages: 33281-33289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inter-α-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex.2004

    • Author(s)
      L.Zhuo, V.C.Hascall, K.Kimata
    • Journal Title

      J Biol Chem 279

      Pages: 38079-38082

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hyaluronan synthase expression in ovarian cancer.2004

    • Author(s)
      H.Yabushita, M.Noguchi, T.Kishida, K.Fusano, Y.Noguchi, N.Itano, K.Kimata
    • Journal Title

      Oncol Rep 12

      Pages: 739-743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction.2004

    • Author(s)
      M.Yoshida, S.Sai, K.Marumo, T.Tanaka, N.Itano, K.Kimata, K.Fujii
    • Journal Title

      Arthritis Res Ther 6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chapter 9 Biological function of SHAP-hyaluronan covalent complex.2004

    • Author(s)
      L.Zhuo, N.Itano, L.Shen, J.Wu, H.Watanabe, K.Kimata, T.Nonogaki
    • Journal Title

      Chemistry and Biology of Hyaluronan (Elsevier Ltd.)

      Pages: 205-222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo hyaluronan synthesis by expression of mammalian hyaluronan synthase gene in drosophrla.2004

    • Author(s)
      S.Takeo, M.Fujise, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 18920-18925

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inter-α -trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex.2004

    • Author(s)
      L.Zhuo, V.C.Hascall, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 38079-38082

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcrintase oolvmerase chain reaction.2004

    • Author(s)
      M.Yoshida, S.Sai, et al.
    • Journal Title

      Arthritis Res Ther 6

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular heterogeneity of the SHAP-hyaluronan complex. Isolation and characterization of the complex in synovial fluid from patients with rheumatoid arthritis.2003

    • Author(s)
      W.Yingsung, L.Zhuo, et al.
    • Journal Title

      J Biol Chem 278

      Pages: 32710-32718

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Secretion of macrophage urokinase plasminogen activator is dependent on proteoglycans.2003

    • Author(s)
      G.Pejler, J.O.Winberg, et al.
    • Journal Title

      Eur J Biochem 270

      Pages: 3971-3980

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A physiological function of serum proteoglycan bikunin : The chondroitinsulfate moiety plays a central role.2003

    • Author(s)
      L.Zhuo, A.Salustri, et al.
    • Journal Title

      Glycoconj J 19

      Pages: 241-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A study of inter-alpha-trypsin inhibitor chains expression in liposarcomas.2003

    • Author(s)
      I.Tabata, Y.Nishida, et al.
    • Journal Title

      Eur J Surg Oncol 29

      Pages: 665-669

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct interaction of versican/PG-M with hyaluronan and link protein.2003

    • Author(s)
      K.Matsumoto, M.Shionyu, et al.
    • Journal Title

      J Biol Chem 278

      Pages: 41205-41212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] CD44 variant exon 6 expressions in colon cancer assessed by quantitative analysis using real time reverse transcriptase-polymerase chain reaction.2003

    • Author(s)
      Y.Yamada, N.Itano, et al.
    • Journal Title

      Oncol Rep 10

      Pages: 1919-1924

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The proteoglycan aggregate : Structure and function.2003

    • Author(s)
      H.Watanabe, K.Matsumoto, et al.
    • Journal Title

      Connective Tissue 35

      Pages: 201-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ヒアルロン酸リッチマトリックスと線維化2003

    • Author(s)
      板野直樹, 木全弘治
    • Journal Title

      現代医療 35

      Pages: 337-342

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] プロテオグリカン2003

    • Author(s)
      渡辺秀人, 木全弘治
    • Journal Title

      蛋白質 核酸 酵素 48

      Pages: 916-922

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ヒアルロン酸合成酵素2003

    • Author(s)
      板野直樹, 木全弘治
    • Journal Title

      蛋白質 核酸 酵素 48

      Pages: 1027-1032

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular heterogeneity of the SHAP-hyaluronan complex. Isolation and characterization of the complex in synovial fluid from patients with rheumatoid arthritis.2003

    • Author(s)
      W.Yingsung, L.Zhuo, M.Morgelin, M.Yoneda, D.Kida, H.Watanabe, N.Ishiguro, H.Iwata, K.Kimata
    • Journal Title

      J Biol Chem 278

      Pages: 32710-32718

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Secretion of macrophage urokinase plasminogen activator is dependent on proteoglycans.2003

    • Author(s)
      G.Pejler, J.O.Winberg, T.T.Vuong, F.Henningsson, L.Uhlin-Hansen, K.Kimata, S.O.Kolset
    • Journal Title

      Eur J Biochem 270

      Pages: 3971-3980

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A physiological function of serum proteoglycan bikunin : The chondroitin sulfate moiety plays a central role.2003

    • Author(s)
      L.Zhuo, A.Salustri, K.Kimata
    • Journal Title

      Glycoconj J 19

      Pages: 241-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A study of inter-alpha-trypsin inhibitor chains expression in liposarcomas.2003

    • Author(s)
      I.Tabata, Y.Nishida, H.Sugiura, L.Zhuo, M.Yoneda, K.Kimata, H.Nakashima, N.Ishiguro
    • Journal Title

      Eur J Surg Oncol 29

      Pages: 665-669

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct interaction of versican/PG-M with hyaluronan and link protein.2003

    • Author(s)
      K.Matsumoto, M.Shionyu, M.Go, K.Shimizu, T.Shinomura, K.Kimata, H.Watanabe
    • Journal Title

      J Biol Chem 278

      Pages: 41205-41212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] CD44 variant exon 6 expressions in colon cancer assessed by quantitative analysis using real time reverse transcriptase-polymerase chain reaction.2003

    • Author(s)
      Y.Yamada, N.Itano, H.Narimatsu, T.Kudo, S.Hirohashi, A.Ochiai, I.Tohnai, M.Ueda, K.Kimata
    • Journal Title

      Oncol Rep 10

      Pages: 1919-1924

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The proteoglycan aggregate : Structure and function.2003

    • Author(s)
      H.Watanabe, K.Matsumoto, K.Kimata.
    • Journal Title

      Connective Tissue 35

      Pages: 201-205

    • NAID

      110003994259

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chapter 4 Functions of proteoglycan/glycosaminoglycan in liver. Extracellular2003

    • Author(s)
      T.Yada, N.Koide, K.Kimata
    • Journal Title

      Matrix and the Liver (Elsevier Science)

      Pages: 55-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mammalian hyaluronan synthases.2002

    • Author(s)
      N.Itano, K.Kimata.
    • Journal Title

      IUBMB Life 54

      Pages: 195-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Abnormal accumulation of hyaluronan matrix diminishes contact-inhibition of cell growth and promotes cell migration.2002

    • Author(s)
      N.Itano, Y.Yamada, et al.
    • Journal Title

      Proc Natl Acad Sci USA 99

      Pages: 3609-3614

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains.2002

    • Author(s)
      H.Takagi, Y.Asano, et al.
    • Journal Title

      J Cell Sci 115

      Pages: 3309-3318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine.2002

    • Author(s)
      M.Gotoh, T.Yada, et al.
    • Journal Title

      J Biol Chem 277

      Pages: 38179-38188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that tansfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis.2002

    • Author(s)
      M.Gotoh, T.Sato, et al.
    • Journal Title

      J Biol Chem 277

      Pages: 38189-38196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mammalian hyaluronan syntheses.2002

    • Author(s)
      N.Itano, K.Kimata
    • Journal Title

      IUBMB Life 54

      Pages: 195-199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Abnormal accumulation of hyaluronan matrix diminishes contact-inhibition of cell growth and promotes cell migration.2002

    • Author(s)
      N.Itano, F.Atsumi, T.Sawai, Y.Yamada, O.Miyaishi, T.Senga, M.Hamaguchi, K.Kimata
    • Journal Title

      Proc Natl Acad Sci USA 99

      Pages: 3609-3614

    • NAID

      110004002486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains.2002

    • Author(s)
      H.Takagi, Y.Asano, N.Yamakawa, I.Matsumoto, K.Kimata
    • Journal Title

      J Cell Sci 115

      Pages: 3309-3318

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine.2002

    • Author(s)
      M.Gotoh, T.Yada, T.Sato, T.Akashima, H.Iwasaki, H.Mochizuki, N.Inaba, A.Togayachi, T.Kudo, H.Watanabe, K.Kimata, H.Narimatsu
    • Journal Title

      J Biol Chem 277

      Pages: 38179-38188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that tansfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis.2002

    • Author(s)
      M.Gotoh, T.Sato, T.Akashima, H.Iwasaki, A.Kameyama, H.Mochizuki, T.Yada, N.Inaba, Y.Zhang, N.Kikuchi, Y-D.Kwon, A.Togayachi, T.Kudo, S.Nishihara, H.Watanabe, K.Kimata, H.Narimatsu
    • Journal Title

      J Biol Chem 277

      Pages: 38189-38198

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The covalent complex formation of hyaluronan with heavy chains of inter- a -trypsin inhibitor family is important for its functions.2002

    • Author(s)
      W.Yingsung, L.Zhuo, M.Yoneda, N.Ishiguro, H.Iwata, K.Kimata
    • Journal Title

      The Many Faces of Osteoarthritis.(Birkhauser)

      Pages: 207-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Chapter 9 Biological function of SHAP-hyaluronan covalent complex.2004

    • Author(s)
      L.Zhon, N.Itano et al.
    • Publisher
      Elsevier Ltd.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Chapter 9 Biological function of SHAP-hyaluronan covalent complex.2004

    • Author(s)
      L.Zhuo, N.Itano, et al.
    • Publisher
      Elsevier Ltd.
    • Related Report
      2004 Annual Research Report
  • [Book] Chapter 4 Functions of proteoglycan/glycosaminoglycan in liver.2003

    • Author(s)
      T.Yada, N.Koide, K.Kimata
    • Publisher
      Elisevier Science
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Proteoglycan enhances the formation of the SHAP-hyaluronan complex and its effect in hyaluronan-rich matrix.2002

    • Author(s)
      M.Zhao, M.Yoneda, et al.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Hyaluronan during liver regeneration after partial hepatectomy : transient accumulation in the tissue and elevation of the serum concentration.2002

    • Author(s)
      T.Yada, H.Maeda, et al.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] The covalent complex formation of hyaluronan with heavy chains of inter-α-trypsin inhibitor family is important for its functions.2002

    • Author(s)
      W.Yingsung, L.Zhuo, et al.
    • Publisher
      Birkhauser
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 妊娠中毒症の検知方法及び検知キット2005

    • Inventor(s)
      野口 昌良, 藪下 廣光, 岸田 畜子, 卓 麗聖, 木全 弘治
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Industrial Property Number
      2005-070896
    • Filing Date
      2005-03-14
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] ピアルロナン結合性タンパク質とピアルロナンとの複合体の形成促進剤2004

    • Inventor(s)
      戸井田敏彦, 木全 弘治
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Filing Date
      2004-01-30
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] ノックアウトマウスを用いた炎症惹起物質のスクリーニング方法2003

    • Inventor(s)
      卓 麗聖, 木全 弘治
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Industrial Property Number
      2003-412433
    • Filing Date
      2003-12-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ヒアルロン酸結合性ペプチド2002

    • Inventor(s)
      松本 和, 木全 弘治, 渡辺 秀人
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Industrial Property Number
      2002-322196
    • Filing Date
      2002-11-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 新規酵素活性測定方法2002

    • Inventor(s)
      卓 麗聖, 木全 弘治
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Industrial Property Number
      2002-382154
    • Filing Date
      2002-12-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] W.Yingsung, L.Zhuo, et al.: "Molecular heterogeneity of the SHAP-hyaluronan complex. Isolation and characterization of the complex in synovial fluid from patients with rheumatoid arthritis"J Biol Chem. 278. 32710-32718 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] G.Pejler, J.O.Winberg, et al.: "Secretion of macrophage urokinase plasminogen activator is dependent on proteoglycans"Eur J Biochem. 270. 3971-3980 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] L.Zhuo, A.Salustri, et al.: "A physiological function of serum proteoglycan bikunin : The chondroitinsulfate moiety plays a central role"Glycoconj J. 19. 241-247 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.Tabata, Y.Nishida, et al.: "A study of inter-alpha-trypsin inhibitor chains expression in liposarcomas"Eur J Surg Oncol. 29. 665-669 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Matsumoto, M.Shionyu, et al.: "Distinct interaction of versican/PG-M with hyaluronan and link protein"J Biol Chem. 278. 41205-41212 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Yamada, N.Itano, et al.: "CD44 variant exon 6 expressions in colon cancer assessed by quantitative analysis using real time reverse transcriptase-polymerase chain reaction"Oncol Rep. 10. 1919-1924 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Watanabe, K.Matsumoto, et al.: "The proteoglycan aggregate : Structure and function"Connective Tissue. 35. 201-205 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Yamada, N.Itano, et al.: "Differential regulation by IL-1β and EGF of expression of three different hyaluronan syntases in oral mucosa epithelial cells and fibroblasts and dermal fibroblasts : Quantitative anylysis using real time RT-PCR"J Invest Dermatol. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Yamada, N.Itano, et al.: "Elevated transcript level of hyaluronan synthase 1 gene correlates with poor prognosis of human colon cancer"Clin Exp Metastasis. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Itano, T.Sawai, et al.: "Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation"J Biol Chem. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Takeo, M.Fujise, et al.: "In vivo hyaluronan synthesis by expression of mammalian hyaluronan synthase gene in drosophila"J Biol Chem. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Cattaruzza, M.Schiappacassi, et al.: "The globular domains of PGM/versican modulate the proliferation-apoptosis equilibrium and invasive capabilities of tumor cells"FASEB J. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 板野直樹, 木全弘治: "ヒアルロン酸リッチマトリックスと線維化"現代医療. 35. 337-342 (2003)

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      2003 Annual Research Report
  • [Publications] 渡辺秀人, 木全弘治: "プロテオグリカン"蛋白質 核酸 酵素. 48. 916-922 (2003)

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      2003 Annual Research Report
  • [Publications] 板野直樹, 木全弘治: "ヒアルロン酸合成酵素"蛋白質 核酸 酵素. 48. 1027-1032 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yada, N.Koide, K.Kimata.: "Chapter 4 Functions of proteoglycan/glycosaminoglycan in liver."Extracellular Matrix and the Liver.Elsevier Science. 55-74 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Itano, K.Kimata.: "Mammalian hyaluronan synthases"IUBMB Life. 54. 195-199 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Itano, Y.Yamada, et al.: "Abnormal accumulation of hyaluronan matrix diminishes contact-inhibition of cell growth and promotes cell migration"Proc Natl Acad Sci USA. 99. 3609-3614 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Takagi, Y.Asano, et al.: "Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains"J Cell Sci. 115. 3309-3318 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Gotoh, T.Yada, et al.: "Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine"J Biol Chem. 277. 38179-38188 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Gotoh, T.Sato, et al.: "Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that tansfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis"J Biol Chem. 27. 38189-38196 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] W.Yingsung, L.Zhuo, et al.: "The covalent complex formation of hyaluronan with heavy chains of inter-α-trypsin inhibitor family is important for its functions"The Many Faces of Osteoarthritis Birkhauser. 207-212 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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