• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and function of the heme-based gas sensor proteins

Research Project

Project/Area Number 14380303
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionOkazaki National Research Institutes

Principal Investigator

AONO Shigetoshi  Okazaki National Research Institutes, Center for Integrative Bioscience, Professor, 統合バイオサイエンスセンター, 教授 (60183729)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥11,800,000 (Direct Cost: ¥11,800,000)
KeywordsSensor Protein / Aerotaxis Control System / Hemoprotein / Oxygen Sensor protein
Research Abstract

HemAT-Bs is a heme-containing signal transducer protein responsible for aerotaxis of Bacillus subtilis. The recombinant HemAT-Bs expressed in Escherichia coli was purified as the oxy form in which oxygen was bound to the ferrous heme. Oxygen binding and dissociation rate constants were determined to be kon = 32 μM^<-1> s^<-1> and koff = 23^<-1>, respectively, revealing that HemAT-Bs has a moderate oxygen affinity similar to that of sperm whale myoglobin (Mb). The rate constant for autoxidation at 37 ℃ was 0.06 h^<-1>, which is also close to that of Mb. Although the electronic absorption spectra of HemAT-Bs were similar to those of Mb, HemAT-Bs showed some unique characteristics in its resonance Raman spectra. Oxygen-bound HemAT-Bs gave the Fe-0_2 band at a noticeably low frequency (560 cm^<-1>), which suggests a unique hydrogen bonding between a distal amino acid residue and the proximal atom of the bound oxygen molecule. Deoxy HemAT-Bs gave the Fe-His band at a higher frequency (225 cm<-1>) than those of ordinary His-coordinated deoxy heme proteins. CO-bound HemAT-Bs gave the Fe-CO and C-O bands at 494 and 1964 cm1, respectively, which fall on the same C-O versus Fe-CO correlation line as that of Mb. Based on these results, the structural and functional properties of HemAT-Bs has been elucidated.
In this work, a new CooA homologue from C.hydrogenoformans (Ch-CooA) was also studied. Ch-CooA also contains a protoheme as the active site for sensing CO. The heme in Ch-CooA is six-coordinate, low spin state in the ferric, ferrous and CO-bound forms. The heme environmental structure of Ch-CooA was elucidated by mutagenesis and spectroscopic studies.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 青野重利: "ヘムを活性中心とするセンサータンパク質の構造と機能"生物物理. 45・5. 230-235 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青野重利: "一酸化炭索による遺伝子発現制御:COセンサーとして機能する転写調節因子CooAの構造と機能"化学と生物. 40・3. 206-210 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis"J.Biol.Chem.. 277・16. 13528-13538 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青野重利: "微生物におけるCOの受容システム"医学のあゆみ. 201・8. 533-536 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青野重利: "一酸化炭棄センサーとして機能する転写調節因子CooAの構造と機能"Molecular Medicine. 40・2. 158-164 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Biochemical and Biophysical Properties of the CO-sensing transcriptional Activator CooA"Acc.Chem.Res.. 36・11. 825-831 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Sawai: "Characterization of the heme environmental structure of Cytoglobin, a Fourth globin in humans"Biochemistry. 42・17. 5133-5142 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Resonance Raman and Ligand Binding Analysis of the Oxygen-sensing Signal Transducer Protein HemAT from Bacillus subtilis"Methods in Enzymology. 381. 618-628 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Resonance Raman and ligand binding analysis of the oxygen-sensing, signal transducer protein HemAT from Bacillus subtilis"Methods in Enzymol.. 381. 618-628 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Sawai: "Characterization of the heme environmental structure of Cytoglobin, a fourth globin in human"Biochemistry. 42. 5133-5142 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Biochemical and biophysical properties of the CO-sensing transcriptional activator CooA"Acc.Chem.Res.. 36. 825-831 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Structure and function of the heme-based CO sensor protein CooA (in Japanese)"Molecular Medicine. 40. 158-164 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "CO receptor system in microorganisms (in Japanese)"Igaku no Ayumi.. 201. 533-536 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein Hem AT from Bacillus subtilis"J.Biol.Chem.. 277. 13528-13538 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Regulation of gene expression by CO : Structure and function of CO-sensing transcriptional regulator CooA (in Japanese)"Chemistry and Biology. 40. 206-210 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigetoshi Aono: "Structure and function of the heme-based sensor proteins (in Japanese)"Seibutsu Butsuri.. 45. 230-235 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青野重利: "一酸化炭素センサーとして機能する転写調節因子CooAの構造と機能"Molecular Medicine. 40・2. 158-164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigetoshi Aono: "Biochemical and Biophysical Properties of the CO-sensing transcriptional Activator CooA"Acc.Chem.Res.. 36・11. 825-831 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hitomi Sawai: "Characterization of the heme environmental structure of Cytoglobin, a Fourth globin in humans"Biochemistry. 42・17. 5133-5142 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigetoshi Aono: "Resonance Raman and Ligand Binding Analysis of the Oxygen-sensing Signal Transducer Protein HemAT from Bacillus subtilis"Methods in Enzymology. 印刷中. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 青野重利: "ヘムを活性中心とするセンサータンパク質の構造と機能"生物物理. 45・5. 230-235 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 青野重利: "一酸化炭素による遺伝子発現制御 : COセンサーとして機能する転写調節因子CooAの構造と機能"化学と生物. 40・3. 206-210 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigetoshi Aono: "Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis"J. Biol. Chem.. 277・16. 13528-13538 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 青野重利: "微生物におけるCOの受容システム"医学のあゆみ. 201・8. 533-536 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 青野重利: "一酸化炭素センサーとして機能する転写調節因子CooAの構造と機能"Molecular Medicine. 40・2. 158-164 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi