Molecular neurobiological studies on the taste reception in Drosophila.

• Project/Area Number: 14380329
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kyushu University
• Principal Investigator: TANIMURA Teiichi KYUSHU UNIVERSITY, Faculty of Science, Associate Professor, 大学院・理学研究院, 助教授 (20142010)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Akira KYUSHU UNIVERSITY, Research and Development Center for Highter Education, Research Associate, 高等教育総合開発研究センター, 助手 (40229539)
• Project Period (FY): 2002 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥13,600,000 (Direct Cost: ¥13,600,000); Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000); Fiscal Year 2003: ¥4,400,000 (Direct Cost: ¥4,400,000); Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
• Keywords: Drosophila / gustation / receptor neuron / electrophysiology / bitter taste / 味覚受容体

Research Abstract

In Drosophila, gustatory receptor neurons (GRNs) occur within hair-like structures called sensilla located on prothoracic legs and labellum. Most taste sensilla house four GRNs, which have been named according to their preferred sensitivity to basic stimuli : water (W cell), sugars (S cell), salt at low concentration (L1 cell), and salt at high concentration (L2 cell). We showed for the first time that several taste sensilla on the prothoracic legs detect bitter compounds both through the activation of specific taste neurons but also through inhibition of taste neurons activated by sugars and water. Using a new statistical approach based on the analysis of interspike intervals, we showed that bitter compounds activate the L2 cell. Their sensitivity to bitter compounds was different between sensilla, suggesting that specific receptors to bitter compounds are differentially expressed among L2 cells. Labellar taste sensilla are classified into three types, l-,s-, and i-type, according to their length and location. We recorded the nerve responses from all the three types of sensilla to sugars, salts and bitter compound. We found that s- and i-type sensilla respond to bitter compounds. l- and s-type labellar sensilla possess four cells, while most i-type sensilla house only two GRNs. In i-type sensilla, we demonstratd that the first GRN responds to sugar and to low concentrations of salt. The second GRN detects a range of bitter compounds, among which strychnine is the most potent ; and also to salt at high concentrations. Neither type of GRN responds to water

Research Products

• [Journal Article] Molecular neurophysiology of taste in Drosophila. (2004)
  • Author(s): Ishimoto, H., Tanimura, T.
  • Journal Title: Cell.Mol.Life Sci. 61 Pages: 10-18
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular clearance of ataxin-3 is regulated by a mammalian E4. (2004)
  • Author(s): Matsumoto M., Yada, M., Hatakeyama, S., Ishimoto, H., Tanimura, T., Tsuji, S., Kakizuka, A., Kitagawa, M., Nakayama, K.-I.
  • Journal Title: EMBO J. 23 Pages: 659-669
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Two antagonistic gustatory receptor neurons responding to sweet-salty and bitter taste in Drosophila. (2004)
  • Author(s): Hiroi, M, Meunier, N., Marion-Poll, F., Tanimura, T.
  • Journal Title: J.Neurobiol. 61 Pages: 333-342
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light. (2004)
  • Author(s): Yoshii, T., Funada, Y., Ibuki, T., Matsumoto, A., Tanimura, T., Tomioka, K.
  • Journal Title: J.Insect Physiol. 50 Pages: 479-488
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular clearance of ataxin-3 is regulated by a mammalian E4. (2004)
  • Author(s): Matsumoto, M., Yada, M., Hatakeyama, S., Ishimoto, H., Tanimura, T., Tsuji, S., Kakizuka, A., Kitagawa, M., Nakayama, K.-I.
  • Journal Title: EMBO J. 23 Pages: 659-669
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two antagonistic gustatory receptor neurons responding to sweet-salty and bitter taste in Drosophila. (2004)
  • Author(s): Hiroi, M, Meunier, N., Marion-Poll, F., Tanimura.T.
  • Journal Title: J.Neurobiol. 61 Pages: 333-342
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light. (2004)
  • Author(s): Yoshii, T., Funada, Y., Ibuki, T., Matsumoto, A., Tanimura, T., Tomioka, K.
  • Journal Title: J.Insect hysiol. 50 Pages: 479-488
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular neurophysiology of taste in Drosophila. (2004)
  • Author(s): Ishimoto, H., Tanimura, T.
  • Journal Title: Cellular and Molecular Life Science 61 Pages: 10-18
• [Journal Article] Molecular clearance of ataxin-3 is regulated by a mammalian E4. (2004)
  • Author(s): Matsumoto, M., Yada, M., Hatakeyama, S., Ishimoto, H., Tanimura, T., Tsuji, S., Kakizuka, A., Kitagawa, M., Nakayama, K.-I.
  • Journal Title: EMBO Journal 23 Pages: 659-669
• [Journal Article] Two antagonistic gustatory receptor neurons responding to sweet-salty and bitter taste in Drosophila. (2004)
  • Author(s): Hiroi, M, Meunier, N., Marion-Poll, F., Tanimura, T.
  • Journal Title: Journal of Neurobiology 61 Pages: 333-342
• [Journal Article] Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light. (2004)
  • Author(s): Yoshii, T., Funada, Y., Ibuki, T., Matsumoto, A., Tanimura, T., Tomioka, K.
  • Journal Title: Journal of Insect Physiology 50 Pages: 479-488
• [Journal Article] Peripheral coding of bitter taste in Drosophila. (2003)
  • Author(s): Meunier, N., Marion-Poll, F., Rospars, J-P, Tanimura, T.
  • Journal Title: J.Neurobiol. 56 Pages: 139-152
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of PDF-expressing cells, possible clock neurons, in the housefly Musca domestics. (2003)
  • Author(s): Pyza, E., Siuta, T., Tanimura, T.
  • Journal Title: Micros Res.Techniq. 62 Pages: 103-113
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of PDF-expressing cells, possible clock neurons, in the housefly Musca domestica. (2003)
  • Author(s): Pyza, E., Siuta, T., Tanimura, T.
  • Journal Title: Micros Res.Techniq. 62 Pages: 103-113
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genome-wide transcriptional orchestration of circadian rhythms in Drosophila. (2002)
  • Author(s): Ueda, H.R., Matsumoto, A., Kawamura, M., Iino, M., Tanimura, T., Hashimoto, S.
  • Journal Title: J.Biol.Chem. 277 Pages: 14048-14052
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differentiated nerve response to sugars among labellar chemosensilla in Drosophila. (2002)
  • Author(s): Hiroi, M., Marion-Poll, F., Tanimura, T.
  • Journal Title: Zool.Sci. 19 Pages: 1009-1018
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps. (2002)
  • Author(s): Hayashi, S., Ito, K., Sado, S., Taniguchi, M., Takeuchi, H., Aigaki, T., Matsuzaki, F., Nakagoshi, H., Tanimura, T., Ueda, R., Uemura, T., Yoshihara, M., Goto, S.
  • Journal Title: Genesis 34 Pages: 58-61
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The period gene and allochronic reproductive isolation in Bactrocera cucurbitae. (2002)
  • Author(s): Miyatake, T., Matsumoto, A., Matsuyama, T., Ueda, H., Toyosato, T., Tanimura, T.
  • Journal Title: Proc.R.Soc.Lond.B 269 Pages: 2467-2472
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] GETDB, a database compiling expression patterns and molecular locations of a collection of Ga14 enhancer traps. (2002)
  • Author(s): Hayashi, S., Ito, K., Sado, S., Taniguchi, M., Takeuchi, H., Aigaki, T., Matsuzaki, F., Nakagoshi, H., Tanimura, T., Ueda, R., Uemura, T., Yoshihara, M., Goto, S.
  • Journal Title: Genesis 34 Pages: 58-61
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Meunier, N., et al.: "Peripheral coding of bitter taste in Drosophila"J.Neurobiol.. 56. 139-152 (2003)
• [Publications] Pyza, E. et al.: "Development of PDF-expressing cells, possible clock neurons, in the housefly, Musca domestica"Micros Res.Techniq.. 62. 103-113 (2003)
• [Publications] Ishimoto, H., Tanimura, T.: "Molecular neurophysiology of taste in Drosophila"Cell.Mol.Life Sci.. 61. 10-18 (2004)
• [Publications] Ueda, H.R.et al.: "Genome-wide transcriptional orchestration of circadian rhythms in Drosophila"J. Biol. Chem. 277. 14048-14052 (2002)
• [Publications] Hiroi, M. et al.: "Differentiated nerve response to sugars among labellar chemosensilla in Drosophila"Zool. Sci.. 19. 1009-1018 (2002)
• [Publications] Hayashi, S. et al.: "GETDB, a database compiling expression patterns and molecular locations of a collection of Ga14 enhancer traps"Genesis. 34. 58-61 (2002)
• [Publications] Miyatake, T. et al.: "The period gene and allochronic reproductive isolation in Bactrocera cucurbitae"Proc. R. Soc. Lond. B. 269. 2467-2472 (2002)