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Molecular genetic studies of asymmetric cell division in C.elegans

Research Project

Project/Area Number 14380349
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionRIKEN

Principal Investigator

SAWA Hitoshi  RIKEN, Laboratory for Cell Fate Decision, Team Leader, 細胞運命研究チーム, チームリーダー (80222024)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 2003: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsasymmetric cell division / C.elegans / Wnt / cell polarity / SWl / SNF complex / Mediator complex / cyclin E / microtubule / C.elegans / Frizzled / SNF / サイクリンE / 微小管 / Meis / Pbx / Hox / psa-3
Research Abstract

Asymmetric cell division is a fundamental mechanism to produce cellular diversity during development. In C.elegans, asymmetries of many cell divisions are regulated by the Wnt signaling pathway. We are focusing on asymmetric division of the T cell during postembryonic development. We screened for mutants that disrupt asymmetry of the T cell division and identified 101 mutants. We have mapped these mutations on each chromosome using SNP (single nucleotide polymorphisms) and performed complementation tests. The results suggests that they are mutations of 51 different genes including those which was identified previously. Our results show that a large number of genes are involved in an asymmetric division of a single cell.
We cloned some of the genes we have identified and analyzed their functions. psa-1O and psa-13 encodes homologs of BAF25O and BAF53 that are components of the SWl/SNF complex. cic-1 and cdk-8 encode a homolog of cyclin C and CDK8 that are components of the transcriptional Mediator complex. We have also shown that these genes regulate asymmetric gene expression in daughter cells after the asymmetric division. We also showed that rmd-1 encodes a novel protein with multiple coiled-coil domains. There are multiple homologs of RMD-1 in mammals whose functions have not been reported. Analyses of embryonic function of rmd-1 revealed that rmd-1 is involved in many microtubule-based processes, such as spindle orientation, cytokinesis and cell polarity in C.elegans embryo.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Zhao, X., Sawa, H., Herman, M.A.: "tc1-2 encodes a novel protein that acts synergistically with Wnt signaling pathways in C. elegans"Developmental Biology. 256. 276-289 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhao, X., Sawa, H., Herman, M.A.: "tcl-2 encodes a novel protein that acts synergistically with Wnt signaling pathways in C.elegans"Developmental Biology. 256. 276-289 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhao, X., Sawa, H., Herman, M.A.: "tcl-2 encodes a novel protein that acts synergistically with Wnt signaling pathways in C.elegans"Developmental Biology. 256. 276-289 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhao, X., Sawa, H., Herman, M.A.: "tcl-2 encodes a novel protein that acts synergistically with Wnt signaling pathways in C. elegan"Developmental Biology. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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