| Project/Area Number |
14380360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Yokohama City University |
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Principal Investigator |
GOSHIMA Yoshio Yokohama City Univ., Sch.of Med., Professor, 医学研究科, 教授 (00153750)
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| Co-Investigator(Kenkyū-buntansha) |
KATURA Isao National Institute of Genetics, Professor, 教授 (00107690)
OGURA Ken-ichi Yokohama City University, Sch.of Med., Assistant Professor, 医学部, 助手 (20326028)
NAKAMURA Fumio Yokohama City Univ., Sch.of Med., Assistant Professor, 医学部, 講師 (10262023)
衣斐 督和 横浜市立大学, 医学部, 助手 (10336539)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2003: ¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 2002: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | L-DOPA / transmitter / receptor / G-protein / C.elegns / pumping behavior / chemosensory / Xenopus / L-ドーパ / 神経伝達物質 / GPCR / 知覚神経 / G蛋白連関型受容体 / アミノ酸 / 生理活性物質 / パーキンソン病 / ドパミン |
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| Research Abstract |
We have identified a candidate receptor for L-DOPA (CeDOPAR) in C.elegans. CeDOPAR share homology with G protein-coupled receptor. In CeDOPAR-expressed Xenopus laevis oocyte, both L-DOPA (pM order) and L-theo-DOPS (mM order) induced inward current. DOPS-induced response was inhibited by DOPA-CHE, an antagonist for L-DOPA, The immunohistochemistry with specific antibody raised against CeDOPAR, suggests that CeDOPAR expression was regulated at different stages. In adult, CeDOPAR was localized near M1 that is thought to control pharyngeal pumping of the worm. On the dopa-containing agar plate, pumping rate of worm was significantly increased when compared that on control agar plate. RNAi for CeDOPAR suppressed the pumping response to DOPA, thereby indicating that CeDOPAR was a candidate receptor mediating the DOPA-induced behavior in nematode. We are now searching various ligands for CeDOPAR.
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