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Molecular mechanisms for olfactory signal transduction

Research Project

Project/Area Number 14380376
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionOsaka University

Principal Investigator

KURAHASHI Takashi  Osaka University, the Department of Biological Sciences, Professor, 大学院生命機能研究科, 教授 (90225251)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Hiroko  Osaka University, The Department of Frontier Biosciences, Research Associate, 大学院生命機能研究科, 助手 (10324823)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordscAMP / olfaction / vapor / adaptation / ion channel / signal transduction / non-linear amplification / caged compound / 嗅覚繊毛 / リアルタイム定量実験 / 電流 / におい物質 / 嗅覚 / シグナルトランスダクション / セカンドメッセンジャー / IP_3 / パッチクランプ法 / エッセンシャルオイル / 臭細胞 / 電気生理学 / 神経 / 脳 / イオンチャネル / 匂い / signal transduction
Research Abstract

Spike discharges of single olfactory receptor cells (ORCs) were recorded with the whole-cell patch clamp method applied to the slice preparation. In parallel, activities of transduction channels were measured under the voltage-clamp condition. Current injection induced repetitive discharges. The firing frequency was dependent on the amount of injected current, showing saturation. When cells ware stimulated by odorants, 54 out of 306 cells exhibited inward currents in response to cineole under the voltage-clamp condition (holding potential =-60 mV). The amplitude of the inward current was dependent on the stimulus period, reflecting the time-integration for the stimulus dose. The dose-response relation could be fitted by the Hill equation. Under the current-clamp condition, odorants induced repetitive spikes. In those cells spike frequency was dose-dependent, expressing saturation similar to the result obtained by current injection. Since both transduction channel and spike generative steps expressed saturation in their dose dependences, we explored what step(s) actually determines saturation in ORCs' signaling processes. By examining dose-response relations of both the current and spikes in the same cells, saturating dose was found to be dependent largely on that of the transduction step. This suggests that the dynamic range is strongly influenced by the transduction system. A simple model derived from the non-linearity of the membrane could explain that the steepness of the depolarization-concentration curve became bigger as Hill coefficient of the transduction channel was increased. In addition, it was speculated that a critical level of dynamic range was, at least in part, modified by the membrane non-linearity.
In addition we made experiments on identifying second messenger mediating olfactory transduction, mechanisms for cross-adaptation and some physiological measurements from human subjects regarding olfactory perception.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (10 results)

All 2004 Other

All Journal Article (3 results) Book (2 results) Publications (5 results)

  • [Journal Article] Bhc-cNMPs as either water-soluble or membrane-permeant photo-releasable cyclic nucleotides for both one and two-photon excitations2004

    • Author(s)
      T.Furuta ら
    • Journal Title

      ChemBiolChem 5

      Pages: 1119-1128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bhc-cNMPs as either water-soluble or membrane-permeant photo-releasable cyclic nucleotides for both one and two-photon excitations

    • Author(s)
      Toshiaki Furuta et al.
    • Journal Title

      ChemBioChem 印刷中

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mechanisms Determining the Dynamic Range of the Bullfrog Olfactory Receptor Cell

    • Author(s)
      Akihiro Tomaru, Takashi Kurahashi
    • Journal Title

      Journal of Neurophysiology 印刷中

    • Related Report
      2004 Annual Research Report
  • [Book] 臭覚生理学2004

    • Author(s)
      倉橋 隆
    • Total Pages
      176
    • Publisher
      フレグランスジャーナル社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 嗅覚生理学2004

    • Author(s)
      倉橋 隆
    • Total Pages
      176
    • Publisher
      フレグランスジャーナル社
    • Related Report
      2004 Annual Research Report
  • [Publications] HIROKO TAKEUCHI: "CROSS-ADAPTATION BETWEEN OLFACTORY RESPONSES INDUCED BY TWO SUBGROUPS OF ODORANT MOLECULES"The Journal of General Physiology. Volume122 Number3. 255-264 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] HIROKO TAKEUCHI: "IDENTIFICATION OF SECOND MESSENGER MEDIATING SIGNAL TRANSDUCTION IN THE OLFACTORY RECEPTOR CELL"The Journal of General Physiology. Volume122 Number5. 557-567 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 竹内裕子: "ユーカリ・エッセンシャルオイルはどのように嗅覚受容細胞を興奮させるか"AROMA RESEARCH NO.16. Volume4 Number4. 336-343 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kyoko WATANABE, Kana UMEZU, Takashi KURAHASHI: "Human Olfactory Contrast Changes during the Menstrual Cycle"Jpn J Physiol. Aug;52(4). 353-359 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeuchi H, Kurahashi T: "Photolysis of caged cyclic AMP in the ciliary cytoplasm of the newt olfactory recentor cell"The Journal of Physiology. 541.3. 825-833 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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