Development of a Phase-Modulation Fluorometer and a Fluorescence-Lifetime Mapping System and ~ Its Application for Tumor Diagnosis Using a Microscope
| Project/Area Number |
14380407
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
IWATA Tetsuo The Univ.of Tokushima, Faculty of Engineering, Prof., 工学部, 教授 (50304548)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Tsutomu Osaka Univ., Graduate School of Engineering Science, Prof., 大学院・基礎工学研究科, 教授 (50136214)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2003: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | Fluorescence lifetime / Phase modulation method / Microscopy / Spatial mapping / Spectroscopic measurement / Tumor diagnosis |
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| Research Abstract |
We have developed a phase-modulation fluorometer and other spectroscopic instruments for obtain specially, spectrally, and temporally resolved information of biological samples. Our final goal is to discriminating a tumor cell from a normal one under a microscope. In order to realize such a system, we have developed the following instrumental techniques : a Fourier-transform phase-modulation fluorometer, a sinusoidally-modulation method of a xenon lamp, a nanosecond-pulse-driven ultraviolet LED, a nanosecond-pulse-driven white LED, a photomultiplier-tube internal-gating method for use in subnanosecond time-resolved spectroscopy, a pseudo-lock-in light detection method for a gain-modulated photomultiplier tube, and so on. In combination with an ultraviolet semiconductor laser and a fluorescence microscope, we have measured fluorescence decay waveform for some biological samples
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Report
(3 results)
Research Products
(10 results)
