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development of model erythrocyte and creation of artificial erythrocyte by using an electro-hydrodynamic spraying operating in cone-jet mode

Research Project

Project/Area Number 14380417
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKansai University

Principal Investigator

OHBA Kenkichi  Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (30029186)

Co-Investigator(Kenkyū-buntansha) URAGAMI Tadashi  Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (80067701)
BANDO Kiyoshi  Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (70156545)
SAKURAI Atsushi  Kansai University, Faculty of Engineering, Lecturer, 工学部, 専任講師 (50162334)
TAJIKAWA Tsutomu  Kansai University, Faculty of Engineering, Research Associate, 工学部, 助手 (80351500)
NAKAMOTO Hiroyuki  Sysmex Co.Ltd., Central Research Institute, Division Head, 中央研究所, 研究企画部長(研究職)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordspolymeric gel / micron-sized gel particle / electro-hydrodynamic spraying method / cone-jet mode / deformability of particle / model erythrocyte / DDS / ultrasound contrast agent / ゲル微粒子 / 柔軟弾塑性粒子 / コーンジェットモードEHD噴霧 / 模擬血小板
Research Abstract

In order to develop model erythrocyte (red blood cell) and to create artificial erythrocyte, we have created tiny, soft and elastic particles of alginic acid gel, of which diameter ranges from about 1 to 10 micrometers. lb do so, we have developed a double spraying method, in which a dilute solution of alginic acid has been made into tiny droplet by a pressurized spraying or an electro-hydrodynamic spraying method, and then these droplets have been chemically reacted with a droplet of calcium chloride by direct contact. This spherical shaped, soft and elastic particle has a size smaller than human erythrocyte, and so can pass smoothly through human blood capillaries.
1.In order to evaluate the elasticity of the polymeric gel particle, we have observed its surface structure in water and have evaluated the local value of Young's modulus by using an atomic force microscope (AFM). As a result, it is observed that its surface is composed of aggregated tiny pebble-like particles from 100 to 2 … More 00 nm in diameter, and that its local Young's modulus is 2.6 to 3.7 MPa, which is much higher than that of human erythrocyte. The overall Young's modulus which have been obtained from the overall deformation of the gel particle is about 0.O5MPa.
2.We have tried to create a micro-sized gel particle containing a bubble inside it. This type of particle will be very useful as an ultrasound contrast agent. We have succeeded in creating such particles, which have an inner structure that a thin membrane of polymeric gel coat a single micron-sized bubble.
3.We have tried to develop alginic acid gel particles containing magnetic fluid as a new drug delivery system (DDS). Three different kinds of gel particle production methods, that is, an impinging 3-fluid spray method, an EHD spray method and a double spray method. As a result, uniformly dispersed aliginic acid gel particles less than 10 μ m in mean diameter, which are containing magnetic fluid, were produced by all the methods. Especially, spherical gel particles of several microns in diameter were produced by the EHD spraying method. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (26 results)

All 2005 2004 2003 Other

All Journal Article (16 results) Patent(Industrial Property Rights) (2 results) Publications (8 results)

  • [Journal Article] DDSとして機能する磁性流体含有高分子ゲル微粒子の創製2005

    • Author(s)
      車野由宗, 大場謙吉, 他
    • Journal Title

      日本機械学会第17回バイオエンジニアリング講演会講演論文集 04-48

      Pages: 43-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Creation of polymeric gel particle containing magnetic fluid as a carrier for the drug delivery system2005

    • Author(s)
      Y.Shano, K.Ohba et al.
    • Journal Title

      Proceedings of JSME No.04-48

      Pages: 43-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DDSとして機能する磁性流体含有高分子ゲル微粒子の創製2005

    • Author(s)
      車野由宗, 大場謙吉, 他
    • Journal Title

      日本機械学会第17回バイオエンジニアリング講演会講演論文集 04・48

      Pages: 43-44

    • Related Report
      2004 Annual Research Report
  • [Journal Article] コーンジェットモードEHD噴霧における微粒化の理論的、実験的検証2004

    • Author(s)
      山川宜之, 大場謙吉, 他
    • Journal Title

      日本機械学会関西支部第79期総会講演会講演論文集 44-1

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 模擬赤血球としての柔軟弾性ゲル微粒子の力学的特性2004

    • Author(s)
      前田憲治, 大場謙吉, 他
    • Journal Title

      第43回日本エム・イー学会大会講演論文集 42・Sup.1

      Pages: 635-635

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] コーンジェットモードEHD噴霧における微粒化機構に関する実験的研究2004

    • Author(s)
      岡村隼次, 大場謙吉, 他
    • Journal Title

      日本機械学会2004年度年次大会講演論文集II 04-1

      Pages: 91-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] ゲル微粒子を用いた超音波造影剤の開発2004

    • Author(s)
      川口正視, 大場謙吉, 他
    • Journal Title

      日本機械学会第15回バイオフロンティア講演会講演論文集 04-39

      Pages: 115-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Theoretrical and experimental investigation on atomization mechanism in an electro-hydrodynamic spraying operating in cone-jet mode2004

    • Author(s)
      T.Yamakawa, K.Ohba et al.
    • Journal Title

      Proceedings of JSME No.44-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanical characteristics of an elasto-flexible polymeric gel particle as a model blood cell2004

    • Author(s)
      K.Maeda, K.Ohba et al.
    • Journal Title

      Proceedings of 43rd Conference of the Japanese Society for Medical and Biological Engineering Vol.42.Sup.1

      Pages: 635-635

    • NAID

      110002484035

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] E experimental investigation on atomization mechanism in an electro-hydrodynamic spraying operating in cone-jet mode2004

    • Author(s)
      J.Okamura, K.Ohba et al.
    • Journal Title

      Proceedings Pt.2 of JSME No.04-1

      Pages: 91-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of ultrasound contrast agent using polymeric gel particle2004

    • Author(s)
      M.Kawaguchi, K.Ohba et al.
    • Journal Title

      Proceedings of JSME No.04-39

      Pages: 115-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 音響放射圧による粒子の移動を利用した気泡と栓子の識別法2004

    • Author(s)
      大場謙吉, 他
    • Journal Title

      第43回日本エム・イー学会大会講演論文集 42・Sup.1

      Pages: 619-619

    • Related Report
      2004 Annual Research Report
  • [Journal Article] マイクロチャンネルアレイを用いた毛細血管中の赤血球の挙動と変形能に関する生体外実験2004

    • Author(s)
      田地川勉, 大場謙吉, 他
    • Journal Title

      可視化情報学会第32回可視化情報シンポジウム講演論文集 24・Sup.1

      Pages: 21-24

    • NAID

      10013272688

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ゲル微粒子を用いた超音波造影剤の開発2004

    • Author(s)
      川口正視, 大場謙吉, 他
    • Journal Title

      日本機械学会第15回バイオフロンティア講演会講演論文集 04・39

      Pages: 115-116

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 噴霧法を用いた高分子ゲル粒子の作製2003

    • Author(s)
      藤原正博, 大場謙吉, 桜井篤, 田地川勉
    • Journal Title

      日本機械学会関西支部第78期定時総会講演会講演論文集 034-1

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of polymeric gel particles using atomizing method2003

    • Author(s)
      M.Fujiwara, K.Ohba, A.Sakurai, T.Tajikawa
    • Journal Title

      Proceedings of JSME No.34-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 微細ゲル粒子の製造方法および製造装置2004

    • Inventor(s)
      大場 謙吉
    • Industrial Property Rights Holder
      学校法人関西大学
    • Industrial Property Number
      2004-070623
    • Filing Date
      2004-03-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] アルギン酸ゲル微粒子の製造方法および製造装置2003

    • Inventor(s)
      大場 謙吉
    • Industrial Property Rights Holder
      学校法人関西大学
    • Industrial Property Number
      2003-076046
    • Filing Date
      2003-03-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 山本宏嗣, 大場謙吉 他: "コーンジェットモードEHD噴霧法による高分子ゲル微粒子の作製"日本混相流学会年会講演会2003講演論文集. 93-94 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 藤原正博, 大場謙吉 他: "噴霧法を用いた高分子ゲル微粒子の作製"日本機械学会2003年度年次大会講演論文集V. No.03-1. 23-24 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 藤原正博, 大場謙吉 他: "2重噴霧法を用いた模擬血球としての高分子ゲル微粒子の作製"第16回バイオエンジニアリング講演会講演論文集. No.03-38. 21-22 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 川口正規, 大場謙吉 他: "ゲル微粒子を用いた超音波造影剤の開発"日本機械学会関西支部第79期総会講演会講演論文集. No.44-1. 9-31-9-32 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山本宏嗣, 大場謙吉 他: "コーンジェットモードEHD噴霧法による高分子ゲル微粒子の作製"日本機械学会関西支部第79期総会講演会講演論文集. No.44-1. 9-39-9-40 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山川宜之, 大場謙吉 他: "コーンジェットモードEHD噴霧における微粒化の理論的、実験的検証"日本機械学会関西支部第79期総会講演会講演論文集. No.44-1. 13-29-13-30 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 尾上明史, 大場謙吉: "模擬赤血球としての柔軟弾性ゲル微粒子の力学的特性"日本機械学会第15回バイオエンジニアリング講演会講演論文集. No.02-35. 373-374 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤原正博, 大場謙吉, 桜井篤, 田地川勉: "噴霧法を用いた高分子ゲル粒子の作製"日本機械学会関西支部第78期定時総会講演会講演論文集. No.034-1. 1-41-1-42 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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