| Project/Area Number |
14380418
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
NAKAMOTO Hiroshi Kawasaki Medical School, Medical Engineering, Research Associate, 医学部, 助手 (10299183)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tokunori Nagoya Medical School, Urology, Assistant Professor, 医学部, 講師 (20182636)
YADA Toyotaka Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥11,700,000 (Direct Cost: ¥11,700,000)
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| Keywords | microcirculation / visualisation / CCD intravital videomicroscope / acute renal failure / blood flow velocity / endotoxin / glomerulus / disseminated intravascular coagulation / 播種性血管内 |
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| Research Abstract |
The purposes of this study are to visualise the transition of disseminated intravascular coagulation(DIC) in the glomerular microcirculation and to evaluate the effects of heparin and an inhibitor of platelet activating factor, CV3988, by measuring blood flow velocity as an index in a rat. To induce DIC, 5mg/kg of lipopolysaccharide(LPS) was administered intravenously to Wistar rats. Glomerular capillary blood flow velocities were measured with our CCD intravital videomicroscope system, which has a spatial resolution of 0.86μm and a time resolution of 33ms.
LPS induced DIC resulting in decrease of glomerular capillary blood flow velocities. Pretreatment with heparin before induction of DIC prevented blood flow velocity decrease approximately by 10%. Pretreatment with CV3988 before induction of DIC prevented blood flow velocity decrease by 90%. Pretreatment with heparin and CV3988 completely abolished the blood flow velocity decrease. Sole administration of either heparin or CV3988 without inducing DIC did not cause any blood flow velocity change at all. CV3988 was more effective than heparin for the blood flow velocity decrease. The pretreatment with both CV3988 and heparin was found to prevent progression of DIC from the aspect of blood flow velocity. This combination treatment is expected to be a new treatment against DIC.
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