| Project/Area Number |
14380419
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | National Cardiovascular Center Research Institute |
|---|
Principal Investigator |
FUJISATO Toshia National Cardiovascular Center Research Institute, Regenerative Medicine & Tissue Engineering, Laboratory Chief (60270732)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KISHIDA Akio Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, 教授 (60224929)
NAKATANI Takeshi National Cardiovascular Center, Organ Transplantation, Director (60155752)
富田 伸司 国立循環器病センター研究所, 再生医療部, 室長 (30260730)
|
|---|
| Project Period (FY) |
2002 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
|
|---|
| Keywords | Regenerative Medicine / Acellular / Scaffold / Tissue Engineering / Cell Transplantation / Tissue Transplantation |
|---|
| Research Abstract |
OBJECTIVES
Tissue engineered grafts based on acellular matrices have been studied to give more durability with growth potential and less immunogenicity to the current bioprostheses. Detergents are commonly used for removal of the cells, whereas the decellularization depends on their permeation in the tissue from its surface and may not be achieved completely in large or hard tissues.
METHODS
Porcine tissues of the heart valves, aorta, cardiac muscle, skeletal muscle, and trachea were isolated under sterile condition. They were treated immediately by cold isostatic pressing (CIP) of 980 Mpa (10,000 atm) followed by washing at 4℃ (PowerGraft treatment). They were then subjected to histological study, porcine endogeneoua retrovirus (PERV) assay, DNA assay, phospholipids assay, and biomechanical study.
RESULTS
The tissues including tracheal cartilage were completely cell free when they were treated by the CIP for 10 min and washing for 7 days. The pressing is applied to the whole of the tissue and no detergents are used in the process. There was no PERV detected from the tissue treated. It has been reported that viruses are mostly inactivated by the CIP more than 600 MPa. The amount of DNA and phospholipids were lower than 1 μg/ml and 0.5 mg/g, respectively. The collagen and elastin fibers were well maintained in the acellular tissue and there were no significant changes in biomechanical properties.
CONCLUSIONS
Porcine cells and PERV were removed completely in a short period by the PowerGraft treatment. This may have more secure acellular tissues for the tissue regeneration.
|
