| Project/Area Number |
14390058
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
HIRANO Seishiro National Institute for Environmental Studies, Environmental Health Sciences Division, Chief, 環境健康研究領域, 室長 (20150162)
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| Co-Investigator(Kenkyū-buntansha) |
CUI Xing National Institute for Environmental Studies, Environmental Health Sciences Division, Researcher, 環境健康研究領域, 研究員 (20342735)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2004: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Oxidative stress / Glutathione / Cytotoxicity / Heme oxigenase-1 / Urban particles / Arsenicals / RNA interference / Single nucleotide polymorphism / 血管内皮細胞 / 細胞II型上皮細胞 / 内皮細胞 / cDNAアレー / ヘムオキシゲナーゼ / 抗酸化酵素 / 感受性 / 粒子状物質 / 砒素 / 肺胞II型上皮細胞 |
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| Research Abstract |
We have addressed cytotoxicity and oxidative-stress potency of organic extracts of diesel exhaust particles (OE-DEP) and urban fine particles (OE-UFP) in rat heart microvessel endothelial (RHMVE) cells. mRNA levels of antioxidant enzymes including heme oxygenase-1 (HO-1) were increased following exposure to OE-DEP and OE-UFP as well as arsenicals. It has been reported that airborne arsenicals generate from coal combustion and cause chronic arsenicism in developing countries. We investigated effects of N-acetylcysteine, buthionine sulfoximine, and siRNA fro HO-1 on cytotoxicity of OE-DEP, OE-UFP, and arsenicals in endothelial cells. N-acetylcysteine did not affect the glutathione levels and reduce the cytotoxicity of arsenicals. Buthionine sulfoximine decreased cellular glutathione level and enhanced the cytotoxicity and expression of HO-1 mRNA. Transfection with siRNA for HO-1 decreased the HO-1 RNA level and enhanced the cytotoxicity of arsenicals except for dialkylarsenicals, suggesting that HO-1 plays a role in antioxidant effects. SNP analyzes were also performed to investigate polymorphism of acetaldehyde dehydrogenase 2.
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