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T ranscriptional regulation of neuron-specific genes by neural restrictive silencer

Research Project

Project/Area Number 14390060
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionNagasaki University (2004)
National Institute for Longevity Sciences,NCGG (2002-2003)

Principal Investigator

MORI Nozomu  Nagasaki University, Graduate School of Biomedical Science, Professer, 大学院・医歯薬学総合研究科, 教授 (00130394)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsNeuron / Differentiation / Gene / Transcription / Silences / 神経遣伝子 / 神経 / 遺伝子 / 基本転写因子 / クロマチン / ダウン症 / プロモーター
Research Abstract

We explored potential roles of the neural restrictive silencing factor NRSF/REST in the following four aspects.
1.Mechanisms of transcriptional repression by NRSF via direct interaction with general transcriptional core factors : We found that NRSF binds directly to TBP, so that NRSF would be recruited to the core promoter region to repress transcription of its target genes at the promoter site (Murai et al.,2004).
2.Structural analysis of the repression domains of NRSF : In collaboration with Prof.Nishimura's group at Yokohama City University, we are in the process of determining the tertiary structure of the two repression domains of NRSF.
3.Determination of the target genes of NRSF : In the promoter region of SCLIP gene, we found a silencer-like element that may contribute to the neuron-specific control of SCLIP. We identified that the N-terminal portion of RB3 contributes to the regulation of microtubule dynamics (Nakao et al.,2004). In collaboration with Prof.Miyata's group at Kagoshima, we identified that the neuronal expression of PACAP gene is under the control of NRSF (Sugawara et al.,2004).
4.Potential roles of NRSF in non-neural tissues : In a subset of non-small cell lung cancer, NRSF was found to be deregulated by loosing the interaction with BRM or BRG1 subunit of the SWI/SNF complex for chromatin remodeling. This work is a part of collaboration with Prof.Iba's group at Tokyo Iniversity (Watanabe et al.,submitted).

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (30 results)

All 2005 2004 Other

All Journal Article (17 results) Publications (13 results)

  • [Journal Article] Hippocampal synaptic modulation for learning and memory by the phosphotyrosine adapter protein ShcC/N-Shc via interaction with the NMDA receptor2005

    • Author(s)
      Miyamoto Y, Chen L, Sato T, Sokabe M, Nabeshima T, Pawson T, Sakai
    • Journal Title

      J.Neurosci. 25(7)

      Pages: 1826-1835

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neural-restrictive silencers in the regulatory mechanism of pituitary adenylate cyclase-activating polypeptide gene expression2005

    • Author(s)
      Sugawara H, Inoue K, Iwata S, Shimizu T, Yamada K, Mori N, Miyata A.
    • Journal Title

      Regulatory Peptide 123(1-3)

      Pages: 1826-1835

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hippocampal synaptic modulation for learning and memory by the phosphotyrosine adapter protein ShcC/N-Shc via interaction with the NMDA receptor.2005

    • Author(s)
      Miyamoto Y, Chen L, Sato T, Sokabe M, Nabeshima T, Pawson T, Sakai R, Mori N
    • Journal Title

      J.Neurosci. 25(7)

      Pages: 1825-1835

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hippocampal synaptic modulation for learning and memory by the phosphotyrosine adapter protein ShcC/N-Shc via interaction with the NMDA receptor2005

    • Author(s)
      Miyamoto Y, Chen L, Sate T, Sekabe M, Nabeshima T, Pawson T, Sakai R, Mori N
    • Journal Title

      J.Neurosci. 25(7)

      Pages: 1826-1835

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct interaction of NRSF with TBP : The chromatin-reorganization and the core promoter repression for neuron-specific gene transcription2004

    • Author(s)
      Murai, K., Naruse, Y., Shaul, Y., Agata.K., Mori, N
    • Journal Title

      Nuc.Acid.Res. 32(10)

      Pages: 3180-3189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Modulation of the stathmin-like microtubule destabilizing activity of RB3, a neural-specific member of the SCG10 family, by its N-terminal domain2004

    • Author(s)
      Nakao, C., Itoh, TJ., Hotani, H., Mori, N
    • Journal Title

      J.Biol.Chem. 279(22)

      Pages: 23014-23021

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Abnormal angiogenesis in Foxo1 (FKHR)-deficient mice2004

    • Author(s)
      Furuyama T, Kitayama K, Motoyama N, Mori N(他9名)
    • Journal Title

      J.Biol.Chem. 279(33)

      Pages: 34741-34749

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neuro-p24 plays an essential role in neurite extension : antisense oligonucleotide inhibition of neurite extension in cultured DRG neurons and neuroblastoma cells2004

    • Author(s)
      Ida M, Suzuki H, Mori N, Taketani S, Araki M
    • Journal Title

      Neurosci.Res. 50

      Pages: 199-208

    • NAID

      10014233690

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hippocampal synaptic modulation for learning and memory by the phosphotyrosine adapter protein ShcC/N-Shc via interaction with the NMDA receptor2004

    • Author(s)
      Sugawara H, Inoue K, Iwata S, Shimizu T, Yamada K, Mori N, Miyata A
    • Journal Title

      Regulatory Peptides 123(1-3)

      Pages: 9-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct interaction of NRSF with TBP : The chromatin-reorganization and the core promoter repression for neuron-specific gene transcription.2004

    • Author(s)
      Murai, K., Naruse, Y., Shaul, Y., Agata, K., Mori, N.
    • Journal Title

      Nuc.Acid Res. 32(10)

      Pages: 3180-3189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Modulation of the stath min-like microtubule destabilizing activity of RB3, a neural-specific member of the SCG10 family, by its N-terminal domain.2004

    • Author(s)
      Nakao, C., Itoh, TJ., Hotani, H., Mori, N.
    • Journal Title

      J.Biol.Chem. 279(22)

      Pages: 23014-23021

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Abnormal angiogenesis in Foxol (FKHE)-deficient mice.2004

    • Author(s)
      Furuyama T, Kitayama K, Shimoda Y, Ogawa M, Sone K, Yoshida-Araki K, Hisatsune H, Nishikawa SI, Nakayama K, Nakayama K, Ikeda K, Motoyama N, Mori N.
    • Journal Title

      J.Biol.Chem. 279(33)

      Pages: 34741-34749

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neuro-p24 plays an essential role in neurite extension : antisense oligonucleotide inhibition of neurite extension in cultured DRG neurons and neuroblastoma cells.2004

    • Author(s)
      Ida M, Suzuki H, Mori N, Taketani S, Araki M.
    • Journal Title

      Neurosd.Res. 50

      Pages: 199-208

    • NAID

      10014233690

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct interaction of NRSF with TBP : The chromatin-reorganization and the core promoter repression for neuron-specific gene transcription2004

    • Author(s)
      Mural, K., Naruse, Y., Shaul, Y., Agata, K., Mori, N
    • Journal Title

      Nuc.Acid.Res. 32(10)

      Pages: 3180-3189

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Abnormal angiogenesis in Foxol (FKHR) -deficient mice2004

    • Author(s)
      Furuyama T, Kitayama K, Motoyama N, Mori N(他9名)
    • Journal Title

      J.Biol.Chem. 279(33)

      Pages: 34741-34749

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neuro-p24 plays an essential role in neurite extension : antisense oligonucl eotide inhibition of neurite extension in cultured DRG neurons and neuro blastoma cells2004

    • Author(s)
      Ida M, Suzuki H, Mori N, Taketani S, Araki M
    • Journal Title

      Neurosci.Res. 50

      Pages: 199-208

    • NAID

      10014233690

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neural-restrictive silencers in the regulatory mechanism of pituitary adenylate cyclase-activating polypeptide gene expression2004

    • Author(s)
      Sugawara H, Inoue K, Iwata S, Shimizu T, Yamada K, Mori N, Miyata A
    • Journal Title

      Regulatory Peptides 123(1-3)

      Pages: 9-14

    • Related Report
      2004 Annual Research Report
  • [Publications] Murai K, Naruse Y 他3名: "Direct interaction of NRSF with TBP : The chromatin-reorganization and the core promoter repression for neuron-specific gene transcription."Nuc.Acid Res.. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sohn SY, Weitzdoerfer R 他2名: "Transcription factor REST dependent proteins are comparable between Down syndrome and control brains : challenging a hypothesis."J.Neural Transm.Suppl.. 67. 59-66 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakao C, Itoh TJ 他2名: "Modulation of the stathmin-like microtubule destabilizing activity of RB3,a neural-specific member of the SCG10 family, by its N-terminal domain."J.Biol.Chem.. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mori N, Zhu W 他5名: "Roles of Shc signaling in oxidative stress response and aging."J.Clin.Biochem.Nutri.. 34. 71-78 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Furuyama T, Kitayama K 他2名: "Forkhead transcription factor FOXO1(FKHR)-dependent induction of PDK4 gene expression in skeletal muscles during energy deprivation."Biochem.J.. 375. 365-371 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wang Z, Kontani Y 他4名: "Muscle type difference in the regulation of UCP3 under cold conditions."Biochem.Biophys.Res.Comm.. 305. 244-249 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mori N, Mizuno T, 他3名: "Effect of age on the gene expression of neural-restrictive silencing factor NRSF/REST"Neurobiol. Aging. 23. 255-262 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ogawa E, Saito Y, 他12名: "Fibronectin signaling stimulates BNP gene transcription by inhibiting neuron-restrictive silencer element dependent repression"Cardiovasc. Res.. 53. 451-459 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tabuchi A, Yamada T, 他4名: "REST4-mediated modulation of REST/NRSF silencing function during BDNF gene promoter activation"Biochem. Biochim. Res. Commun.. 290. 415-420 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hanma M, Namikawa K, 他5名: "Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron"J. Neurochem.. 82. 961-975 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura T, Komiya M, (他6名): "Grit, a GTPase-activiting protein for RhoA and Cdc42, regulates neurite extension through its association with TrkA receptor and N-Shc, CrkL/Crk adapter molecules"Mol. Cell. Biol.. 22(24). 8721-8734 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mori N, Morii H: "SCG 10-related neuronal growth-associated proteins in neural development, plasticity. degeneration and aging"J. Neurosci. Res.. 70(3). 264-273 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mori N (分担執筆): "Catecholamine Research : From Molecular Insights to Clinical Medicine"Nagatsu T and Nabeshima T (編集) Kluwer Academic/Plenum Publishers (出版社). 558 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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