| Project/Area Number |
14405024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
植物保護
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
ARIE Tsutomu Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00211706)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Motoichiro Tottori. University, Faculty of Agriculture, Lecturer, 農学部, 講師 (00183343)
TERAOKA Tohru Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professor, 農学部, 教授 (60163903)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2003: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2002: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | phytopathogenic fungi / ascomycetes / tomato / Fusarium oxysporum / phylogeny / coevolution / Chile / wilt / 子のう菌 / Alternaria alternata / Magnaporthe grisea / 分子進化学的解析 / チリ;アメリカ |
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| Research Abstract |
In December 2002 and March 2004, a tield-stage collection of wild tomatoes (Lycopersicon peruvianum and L.chilense) was made in 0-1000m and 2000-3500m of altitude, respectively, in Region I(Tarapaka) of Republic of Chile. A total of ca, 650 fungal isolates were obtained from fresh leaves, stems, and roots of wild tomatoes and 250 of them have been identified by morphological and molecular criteria. We could find about 20 Fusarium oxysporum among the isolates. These F.oxysporum isolates did not show pathogenicity to any tomato tester-varieties, suggesting that they are non-pathogenic. Interestingly, phylogenetic analysis based on the rhibosomal DNA inter genic spacer (rDNA-IGS) sequences revealed that the non-pathogenic F.oxysporum isolats from wild tomatos are closely related to F.oxysporum f. sp. lycopersici, the pathogen of wilt disease of cultivated tomato (L.esculentum), in F.oxysporum tree. This suggested that (1)the non-pathogenic F.oxysporum on wild tomato is an ancient-relatives of the tomato wilt fungus, (2)the pathogenic F.oxysporum had been deliverd from the non-pathogenic F.oxysporum, (3)tomato wilt pathogen has been coevolved with tomatoes. This study was done under cooperation with Dr.Peever, T. L. of Washington State University and Dr.Kistler, C. of USDA-ARS, Minnesota University.
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