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Establishment of methodology for clinicopathological investigation of viral encephalitis occurring in the South-East Asia

Research Project

Project/Area Number 14406010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Virology
Research InstitutionNAGASAKI UNIYERSITY

Principal Investigator

IWASAKI Takuya  Nagasaki University, Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (90146027)

Co-Investigator(Kenkyū-buntansha) INOUE Satoshi  Nagasaki University, National Institute of Infectious Diseases, Department of Veterinary Medicine, Chief, 獣医科学部, 室長 (90213157)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2003: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥6,600,000 (Direct Cost: ¥6,600,000)
Keywordsencephaltis / myelitis / lyssaviruses / Rhahdoviridae / Nipah virus / enterovirus 71 / virus pathology / immunohistochemistry / 脳炎ウイルス / Oitavirus / 免疫組織化学 / 感染モデル / 狂犬病 / ニパウイルス感染症 / 感染病理 / 中枢神経系 / ウイルス性脳炎 / 東南アジア / ウイルス抗原
Research Abstract

In this study we investigated the methodology and pathogenesis of viral infections of the central nervous system. The main targets were lyssaviruses including rabies viruses, the isolate from an insect bat belonging Rhabdoviridae (Oita virus), nipah virus, and enterovirus 71. We initially studied the methods for identification of these viruses in paraffin sections of formalin-fixed human and animal tissues. We established the antibodies to detect these nucleoproteins or capsid proteins by immunization of recombinant proteins expressed in E.coli and degenerated viral particles to rabbits. After confirmation of these rabbit antiserum reacting with viral proteins in western blot analysis, we performed immunohistochemical examinations of animal tissues which were prepared by inoculation of the viruses. The antibody prepared by immunization of nucleoprotein of rabies virus could recognize the various lyssaviruses in paraffin sections and also was capable to detect the antigens in the imported rabies cases in 1970s. The antibody against the nucleoprotein of Nipah virus could detect the antigen in a human autopsy case in Malaysia but the sensitivity was not so good. Regarding Oita virus we sequenced of the nucleocapsid gene and disclosed the unique position of phylogeny of Rhabdoviridae. We established the animal model of enterovirus 71 using the cynomolgus monkeys through the intravenous way and disclosed the similarity and difference with and from the pathogenicity of poliovirus. These results are valuable for further studies on viral encephaltis, especially occurring in the South-east Asia.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (38 results)

All 2005 2004 2003 2002 Other

All Journal Article (29 results) Publications (9 results)

  • [Journal Article] Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus.2005

    • Author(s)
      Miyachi S
    • Journal Title

      Journal of Neuroscience 25(10)

      Pages: 2547-2556

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The alpha/beta interferon response controls tissue tropism and pathogenicity of poliovirus2005

    • Author(s)
      Ida-Hosonuma M
    • Journal Title

      Journal of Virology 79(7)

      Pages: 4460-4469

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Temperature sensitive mutants of enterovirus 71 show attenuation in cynomolgus monkeys : Implication of temperature sensitivity as a determinant of attenuation.2005

    • Author(s)
      Arita M
    • Journal Title

      Journal of General Virology 86(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus.2005

    • Author(s)
      Miyachi S et al.
    • Journal Title

      Journal of Neuroscience 25-10

      Pages: 2547-2556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The alpha/beta interferon response controls tissue tropism and pathogenicity of poliovirus.2005

    • Author(s)
      Ida-Hosonuma M et al.
    • Journal Title

      Journal of Virology 79-7

      Pages: 4460-4469

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus.2005

    • Author(s)
      Miyachi S, Iwasaki T et al.
    • Journal Title

      Journal of Neuroscience 25/5(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sub-genomic replicons of Tick-bome encephalitis virus.2004

    • Author(s)
      Hayasaka D
    • Journal Title

      Archive of Virology 149(6)

      Pages: 1245-1256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing characteristics of both lyssavirus and vesiculovirus.2004

    • Author(s)
      Iwasaki T
    • Journal Title

      Archive of Virology 149(6)

      Pages: 1139-1154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21.2004

    • Author(s)
      Nagata N
    • Journal Title

      Virology 321(1)

      Pages: 87-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka D
    • Journal Title

      Journal of General Virology 85(4)

      Pages: 1007-1018

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential localization of neurons susceptible to enterovirus 71 and poliovirus type 1 in the central nervous system of cynomolgus monkeys after intravenous inoculation.2004

    • Author(s)
      Nagata N
    • Journal Title

      Journal of General Virology 85(10)

      Pages: 2981-2989

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Productive infection in the murine central nervous system with avian influenza virus A (H5N1) after intranasal inoculation.2004

    • Author(s)
      Iwasaki T
    • Journal Title

      Acta Neuropathologica 108(10)

      Pages: 485-492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Naturally Acquired Plasmodium knowlesi Malaria in Human, Thailand.2004

    • Author(s)
      Jongwutiwes S
    • Journal Title

      Emerging Infectious Diseases 10(12)

      Pages: 2211-2213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sub-genomic replicons of Tick-borne encephalitisvirus.2004

    • Author(s)
      Hayasaka D et al.
    • Journal Title

      Archives of Virology 149-6

      Pages: 1245-1256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing characteristics of both lyssavirus and vesiculovirus2004

    • Author(s)
      Iwasaki T et al.
    • Journal Title

      Archives of Virology 149-6

      Pages: 1139-1154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR_<21>.2004

    • Author(s)
      Nagata N et al.
    • Journal Title

      Virology 321-1

      Pages: 87-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka D et al.
    • Journal Title

      Journal of General Virology 85-4

      Pages: 1007-1018

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential localization of neurons susceptible to enterovirus 71 and poliovirus type 1 in the central nervous system of cynomolgus monkeys after intravenous inoculation.2004

    • Author(s)
      Nagata N et al.
    • Journal Title

      Journal of General Virology 85-10

      Pages: 2981-2989

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Productive infection in the murine central nervous System with avian influenza virus A (H5N1) after intranasal inoculation.2004

    • Author(s)
      Iwasaki T et al.
    • Journal Title

      Acta Neuropathologica 108-10

      Pages: 485-492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Naturally acquired Plasmodium knowlesi malaria in human, Thailand.2004

    • Author(s)
      Jongwutiwes S et al.
    • Journal Title

      Emerging Infectious Diseases 10-12

      Pages: 2211-2213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka D, Iwasaki T, et al.
    • Journal Title

      Journal of General Virology 85/4

      Pages: 1007-1018

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing of characteristics of both lyssavirus and vesculovirus.2004

    • Author(s)
      Iwasaki T, Inoue S, et al.
    • Journal Title

      Archives of Virology 149/6

      Pages: 1139-1157

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential localization of neurons susceptible to enterovirus 71 and poliovirus type 1 in the central nervous system of cynomolgus monkeys after intravenous inoculation.2004

    • Author(s)
      Nagata N, Iwasaki T, et al.
    • Journal Title

      Journal of General Virology 85/10

      Pages: 2981-2989

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Productive infection in the murine central nervous system with avian influenza virus A (H5N1) after intranasal inoculation.2004

    • Author(s)
      Iwasaki T, Itamura S, et al.
    • Journal Title

      Acta Neuropathologica 108

      Pages: 485-492

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific anti-rabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues.2003

    • Author(s)
      Inoue S
    • Journal Title

      Pathology International 83

      Pages: 525-533

    • NAID

      50000285840

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific anti-rabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues.2003

    • Author(s)
      Inoue S et al.
    • Journal Title

      Pathology International 83

      Pages: 523-533

    • NAID

      50000285840

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pyramidal and extrapyramidal involvement in experimeantal infection of cynomolgus monkeys with enterovirus 71.2002

    • Author(s)
      Nagata N
    • Journal Title

      Journal of Medical Virology 67

      Pages: 207-216

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pyramidal and extrapyramidal involvement in experimeantal infection of cynomolgus monkeys with enterovirus 71.2002

    • Author(s)
      Nagata N et al.
    • Journal Title

      Journal of Medical Virology 67

      Pages: 207-216

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Temperature sensitive mutants of enterovirus 71 show attenuation in cynomolgus monkeys: Implication of temperature sensitivity as a determinant of attenuation.

    • Author(s)
      Arita M et al.
    • Journal Title

      Journal of General Virology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Inoue S, Sato Y, Hasegawa H, Noguchi A, Yamada A, Kurata T, Iwasaki T.: "Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific anti-rabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues"Pathology International. 53・8. 525-533 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayasaka D, Yoshii K, Ueki T, Iwasaki T, Takashima I.: "Sub-genomic replicons of tick-borne encephalitis virus"Archives of Virology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwasaki T, Inoue S, Tanaka K, Sato Y, Morikawa S, Hayasaka D, Moriyama M, Ono T, Kanai S, Yamada A, Kurata T.: "Characterization of Oita virus 296/1972 isolated from a horseshoe bat as a new genus of Rhabdoviridae"Archives of Virology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagata N, Iwasaki T, Ami Y, Sato Y, Hatano I, Harashima A, Suzuki Y, Yoshii T, Hashikawa T, Sata T, Horiuchi Y, Koike S, Kurata T, Nomoto A.: "A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21"Virology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayasaka D, Gritsun TS, Yoshii K, Ueki T, Goto A, Mizutani T, Kariwa H, Iwasaki T, Gould EA, Takashima T.: "Amino acid changes responsible for attenuation of viral neurovirulence in an infectious cONA clone of the Oshima strain of Tick-borne encephalitis virus"Journal of General Virology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 岩崎琢也, 永田典代, 清水博之: "エンテロウイルス71脳炎"小児感染免疫. 14(2). 133-138 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岩崎琢也, 清水博之, 永田典代: "エンテロウイルス71"病理と臨床. (印刷予定). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 峰咲幸哲, 本田まりこ, 岩崎琢也, 白木公康: "BALB/cマウスへの単純ヘルペスウイルス2型臨床分離株経膣接種による神経伝達経路と脊髄炎の発生病理"東京慈恵会医科大学雑誌. 117(6). 427-439 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue S, Sato Y, Hasegawa H, Noguchi A, Yamada A, Kurata T, Iwasaki T: "Cross-reactive antigenicity of nucleoproteins of lyssaviruses to a monospecific anti-rabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues"Pathology International. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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