| Project/Area Number |
14406026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hematology
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
MATSUOKA Hiroyuki Jichi Medical School, Departmentc Medical Zoology, Associate Professor, 医学部, 助教授 (10173816)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Shigeto Jichi Medical School, Department of Medical Zoology, Lecturer, 医学部, 講師 (10296121)
ARAI Meiji Jichi Medical School, Department of Medical Zoology, Lecturer, 医学部, 講師 (30294432)
HIRAI Makoto Jichi Medical School, Department of Medical Zoology, Assistant Professor, 医学部, 助手 (50326849)
KAWAMOTO Fumihiko Oita University, Facauty of Medicine, Institute of Scientific Research, Professor, 医学部, 教授 (40115556)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | G6PD / malaria / molecular epidemiology / human genetics / metabolic abnormality / primaquine / anemia / Asia / ベトナム / カンボジア / 韓国 / ネパール / インドネシア / ミャンマー / 中国 |
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| Research Abstract |
We conducted surveies of malaria diagnoses and glucose-6-phosphate dehydrogenase (G6PD) testing in remote areas of Asian countries. We took two drops of blood from the candidates by the finger prick method. One drop of blood was used for malaria diagnosis of Acridine orange method, and another drop of blood was for G6PD test using WST-8. Both diagnoses were completed within 30 minutes. Patients could receive anti-malarial medicine including primaquine when they had malaria parasites and their (G6PD conditions were normal. We obtained a small amount of blood from G6PD deficient subjects as well as informed consents, and analyzed DNA sequenses of G6PD gene. In Myanmar, more than 90% of G6PD deficient subjects showed G6PD Mahidol type (487G>A). However in Cambodia, more than 90% of G6PD deficient subjects showed G6PD Viangchan type (871G>A). These results indicate that both Myanmar people and Cambodian people are independently derived from homogeneous ancestries and different each other in terms of G6PD variant
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