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Development of Cryopreservation Technique and Its Diffusion in South-east Asia for Conservation of Tropical Plant Germplasms Useful for Search for Bioactive Metabolites.

Research Project

Project/Area Number 14406031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Environmental pharmacy
Research InstitutionOsaka University

Principal Investigator

MIYAMOTO Kazuhisa  Osaka University, Graduate School of Pharmaceutical Sciences, Professor, 薬学研究科, 教授 (30028849)

Co-Investigator(Kenkyū-buntansha) HIRATA Kazumasa  Osaka University, Graduate School of Pharmaceutical Sciences, Associate Professor, 薬学研究科, 助教授 (30199062)
NAGASE Hiroyasu  Osaka University, Graduate School of Pharmaceutical Sciences, Research Associate, 薬学研究科, 助手 (00252700)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2003: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsCryopreservation / Conservation of germplasm / Technical diffusion / Microalgae / Encapsulation-dehydration method / International collaboration / Thailand / Indonesia / 植物遺伝資源保護 / 薬用植物
Research Abstract

In this research, we investigated the cryopreservation techniques generally applicable to tropical germplasms of photosynthetic organisms such as higher plants and microalgae useful for searching novel bioactive metabolites in Pharmaceutical Science field. We also attempted to diffuse these techniques in South-east Asian countries in which ecosystem in rainforest is rapidly destructed by environmental pollution many precious species of higher plants and microalgae are on the way to extinction.
As a fundamental research using plant root and green alga, we found a phytohormone, abscisic acid(ABA), could induce tolerance to dehydration and then freezing. ABA-pretreatment increased the survival rates in these materials after freezing in liquid nitrogen. We applied this pretreatment to several plants and algae and succeeded their cryopreservation.
We attempted to search novel bioactive metabolites in microalgae in those areas under cooperation with foreign researchers. Several metabolites showing strong growth inhibitory activities and a biosurfactant could be isolated from freshwater cyanobacteria isolated in Thailand and Indonesia.
Finally, we made efforts to diffuse the developed cryopreservation techniques in Thailand and Indonesia as generally applicable ones for conservation of useful tropical plant and algal germplasms. We visited these countries several times and gave lectures and technical workshops to encourage the consciousness for importance of tropical germplasms and increase the individual techniques of cryopreservation.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2004 2003 2002 Other

All Journal Article (13 results) Publications (4 results)

  • [Journal Article] Generation of reactive oxygen species undergoing redox cycle of Nostocine A : a cytotoxic violet pigment produced by freshwater cyanobacterium Nostoc spongiaeforme.2004

    • Author(s)
      Hirata, K., Yoshitomi, S., Dwi, S., Iwabe, O., Mahakhant, A., Polchai, J., Miyamoto, K.
    • Journal Title

      Journal of Biotechnology 110

      Pages: 29-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phytochelatin synthase : catalytic mechanism and acquisition of its function during molecular evolution.2004

    • Author(s)
      Tsuji, N., Miyamoto, K., Hirata, K.
    • Journal Title

      Recent Rese.Devel.Phytochem. 8

      Pages: 49-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of reactive oxygen species undergoing redox cycle of nostocine A : a cytotoxic violet pigment produced by freshwater cyanobacterium Nostoc spongiaeforme.2004

    • Author(s)
      Hirata, K., Yoshitomi, S., Dwi, S., Iwabe, O., Mahakhant, A., Polchai, J., Miyamoto, K.
    • Journal Title

      J.Biotechnol. 110

      Pages: 29-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phytochelatin synthase : catalytic mechanism and acquisition of its function during molecular evolution.2004

    • Author(s)
      Tsuji, N., Miyamoto, K., Hirata, K.
    • Journal Title

      Recent Res.Devel.Phytochem. 8

      Pages: 49-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of reactive oxygen species undergoing redox cycle of nostocine A, a cytotoxic violet pigment produced by freshwater cyanobacterium Nostoc spongiaeforme.2004

    • Author(s)
      Kazumasa Hirata
    • Journal Title

      Journal of Biotechnology 110・1

      Pages: 29-35

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid.2003

    • Author(s)
      Yoshida, K., Igarashi, E., Mukai, M., Hirata, K., Miyamoto, K.
    • Journal Title

      Plant Cell Envrionment 26

      Pages: 451-457

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bioactivities of nostocine A produced by a freshwater cyanobacterium Nostic spongiaeforme TISTR 8169.2003

    • Author(s)
      Hirata, K., Yoshitomi, S., Dwi, S., Iwabe, O., Mahakhant, A., Polchai, J., Miyamoto, K.
    • Journal Title

      Journal of Bioscience and Bioengineering 95

      Pages: 512-517

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 微細藻類の機能開発と環境保全への応用2003

    • Author(s)
      宮本和久, 平田收正
    • Journal Title

      ファルマシア 39

      Pages: 871-875

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid.2003

    • Author(s)
      Yoshida, K., Igarashi, E., Mukai, M., Hirata, K., Miyamoto, K.
    • Journal Title

      Plant Cell Envrion. 26

      Pages: 451-457

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bioactivities of nostocine A produced by a freshwater cyanobacterium Nostic spongiaeforme TISTR 8169.2003

    • Author(s)
      Hirata, K., Yoshitomi, S., Dwi, S., Iwabe, O., Mahakhant, A., Polchai, J., Miyamoto, K.
    • Journal Title

      J.Biosci.Bloeng. 95

      Pages: 512-517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of microalgal functions and its application to environmental protection.2003

    • Author(s)
      Miyamoto, K., Hirata, K.
    • Journal Title

      Pharmacia 39

      Pages: 871-875

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cryopreservation of hairy root cultures of Vinca minor (L.) by encapsulation-dehydration.2002

    • Author(s)
      Hirata, K., Mukai, M., Goda, S., Ishio-Kinugasa, M., Yoshida, K., Sakai, A., Miyamoto, K.
    • Journal Title

      Biotechnology Letter 24

      Pages: 371-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cryopreservation of hairy root cultures of Vinca minor (L.) by encapsulation-dehydration.2002

    • Author(s)
      Hirata, K., Mukai, M., Goda, S., Ishio-Kinugasa, M., Yoshida, K., Sakai, A., Miyamoto, K.
    • Journal Title

      Biotechnol.Lett. 24

      Pages: 371-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kazumasa Hirata: "Bioactivities of nostocine A produced by freshwater cyanobacterium Nostic spongiaeforme TISTR 8169"Journal of Bioscience and Bioengineering. 95・5. 512-517 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 宮本和久: "微細藻類の機能開発と環境保全への応用"ファルマシア. 39・9. 871-875 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kazumasa Hirata: "Cryopreservation of hairy root cultures of Vinca minor(L.)by encapsulation-dehydration"Biotechnology Letters. 24. 371-376 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kenji Yoshida: "Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid"Plant Cell&Environment. (印刷中).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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