Analyses of aluminum-induced cell death via mitochondrial dysfunction in plant cells.
| Project/Area Number |
14540595
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
YAMAMOTO Yoko Okayama University, Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (50166831)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Aluminum ion / Mitochondria / Cell death / Sucrose-H^+ cotransporter / Calcium ion / Second messenger / Peroxidase / Plant Cells / アルミニウム / カルシウム / 細胞伸長阻害 / ショ糖吸収阻害 / タバコ培養細胞 / 水吸収阻害 / アルミニウム耐性 / 活性酸素 / 細胞骨格 / 植物 / 浸透圧 |
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| Research Abstract |
In acidic soils, aluminum(Al) ion is a major factor to limit plant growth. We have previously found that Al ion on the cell surface triggers mitochondrial dysfunction, reactive oxygen species(ROS) production and eventually cell death. The purpose of this research was to investigate the molecular details of the Al toxicity pathway, and to identify molecules and/or genes related to Al tolerance.
As an important early event, we found that Al inhibits sucrose uptake via sucrose-H^+ cotransporter in cultured tobacco cells. Since the inhibition of sucrose uptake and the inhibition of water uptake were induced simultaneously by Al, we concluded that the Al-induced inhibition of sucrose uptake leads to the inhibition of water uptake and eventually the inhibition of cell elongation. In addition, since the inhibition of sucrose uptake leads to a decrease in the supply of carbon source to mitochondria, which seems to lead to mitochondrial dysfunction and ATP depletion, and finally cell death.
We also found a possibility that transient changes of cytosolic calcium ion concentration are related to Al-stress responses. We finally found that the peroxidase gene overexpressed in Al-tolerant tobacco cells is Al-tolerant gene, and that ATP content is one of determining factors of Al tolerance in pea roots.
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Report
(4 results)
Research Products
(21 results)
